Kooksoondang Brewery Co.

Gyeonggi, South Korea

Kooksoondang Brewery Co.

Gyeonggi, South Korea

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Sang-Jin Lee,Kooksoondang Brewery Co. | Sang-Jin Lee,Sungkyunkwan University | Kim J.-H.,Sungkyunkwan University | Jung Y.W.,Sungkyunkwan University | And 5 more authors.
Korean Journal of Food Science and Technology | Year: 2011

Makgeolli is Korean traditional alcoholic beverage that has historically been brewed. In this study, we analyzed the profile of organic acids in makgeolli and also evaluated its physiological characteristics. Makgeolli contained excess lactic acid, which is produced by lactic acid bacteria (LAB). Anti-obesity effects of makgeolli were investigated in 3T3- L1 preadipocytes. Compared to the negative control, makgeolli inhibited the differentiation of preadipocyte as quantified by Oil red O dye. In particular, 100 μg/mL makgeolli reduced 40 to 70% of differentiation. To evaluate the anti-angiogenic and anti-inflammatory effects of makgeolli, we performed chorioallantoic membrane assay and measured nitric oxide production from lipopolysaccharide- induced RAW264.7 cells. Most makgeolli interrupted the formation of neo-vasculature and significantly inhibited NO production in a dose-dependent manner. Taken together, these findings suggest that commercial makgeolli has inhibitory activities against adipogenesis, neo-vascularization, and inflammation, and also they are influenced by second metabolites from nuruk microflora containing fungi and LAB. © The Korean Society of Food Science and Technology.


Lee S.-J.,Kooksoondang Brewery Co. | Lee S.-J.,Sungkyunkwan University | Kim G.-W.,Kooksoondang Brewery Co.
Journal of Applied Biological Chemistry | Year: 2010

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcriptional factors that belong to nuclear receptors superfamily and are involved in the regulation of lipid metabolism. In the present study, anti-hyperlipidemic properties of n-hexane fraction (RoHe) from cultures of Rhizopus oryzae KSD-815 (R. oryzae KSD-815), which was isolated from Nuruk, were studied. n-Hexane fraction lowered plasma low-density lipoprotein levels and total cholesterol in high cholesterol diet-fed rats, and showed comparable potency compared with that of gemfibrozil. n-Hexane fraction was further fractionated with hexane in increasing EtOAc gradient using cellbased transactivation assay for PPAR-α. The active subfraction 4 (3 g) was applied to octadecyl silica gel column chromatography (φ 4×4.5 cm) and eluted with MeCN-H2O (3:1, 1.8 L) to provide 14 fractions (RoHe-4-1 to RoHe -4-14). Results showed the 2nd-subfractions, RoHe-4-2 and RoHe- 4-6 could activate PPAR-α and PPAR-γ in a dose-dependent manner, and concentrations of fractions RoHe-4-2 and 4-6 to achieve half of GW409544 activity were 12.39 and 13.99 mg/mL, respectively. In functional assay using human hepatoma HepG2 cells, RoHe-4-2 and 4-6 significantly increased apolipoprotein A1 (ApoA1) production and showed more potent stimulation effects compared with fenofibrate. These results indicate that culture of R. oryzae KSD-815 may have a potent hypolipidemic activity through activation of PPAR-α and secretion of ApoA1.


Jung S.K.,Seoul National University | Lim T.G.,Konkuk University | Kim J.E.,Seoul National University | Byun S.,Seoul National University | And 6 more authors.
Food Chemistry | Year: 2012

Consumption of fruits and vegetables is correlated with a lower incidence of cancer. Here, we identified hyperoside as an active compound from Acanthopanax sessiliflorus, and investigated the effect of hyperoside on UVB-induced transactivation of activator protein 1 (AP-1) and on the mitogen-activated protein kinase signalling pathway in JB6 P + cells. Hyperoside inhibited UVB-induced AP-1 transactivation. It inhibited the UVB-induced phosphorylation of p90RSK. Kinase assays revealed that hyperoside significantly inhibited ERK1/2 activity. Furthermore, hyperoside bound to ERK1/2 to suppress its activity. In addition, phosphorylation of cAMP response element binding protein (CREB) and signal transducers and activators of transcription (STAT) 3 were suppressed by hyperoside. Overall, these results indicate that hyperoside may be a promising chemopreventive agent that acts by suppressing the transactivation of AP-1 and the phosphorylation of p90RSK, CREB, and STAT3 through the binding and inhibition of ERK1/2. © 2011 Elsevier Ltd. All rights reserved.


PubMed | Kooksoondang Brewery Co, Republic Services and Andong National University
Type: Journal Article | Journal: Journal of microbiology and biotechnology | Year: 2016

From the lees of bokbunja wine (LBW) made from Rubus coreanus Miquel, we have identified six compounds (1: trans-4-hydroxycinnamic acid; 2: trans-4-hydroxy-3-methoxycinnamic acid; 3: 3,4-dihydroxycinnamic acid; 4: 4-hydroxy-3-methoxybenzoic acid; 5: 3,5-dimethoxy-4- hydroxybenzoic acid; and 6: 3,5-dimethoxy-4-hydroxycinnamic acid (sinapic acid)) through silica gel chromatography and UHPLC-MS. The compounds 1-6 showed strong anticoagulation and platelet aggregation inhibitory activities without hemolytic effect against human red blood cells. To date, this is the first report of the in vitro anti-thrombosis activity of sinapic acid. Our results suggest that different cinnamic and benzoic acid derivatives are closely linked to the anti-thrombosis activity of LBW, and sinapic acid could be developed as a promising anti-thrombosis agent.


Kim M.-S.,Andong National University | Lee Y.-S.,Andong National University | Kim J.S.,Andong National University | Shin W.-C.,Kooksoondang Brewery Co. | Sohn H.-Y.,Andong National University
Korean Journal of Microbiology and Biotechnology | Year: 2014

Sweet potato soju (SPS), a form of traditional distilled alcoholic liquor in Korea, is manufactured by the distillation of fermented broth under normal pressure, thus providing it for a uniquely smooth taste infused with the flavor of sweet potato. After distillation, the lees of SPS is produced as by-product and discarded. In this study, the ethanol and hot water extracts of lees of SPS, and their subsequent organic solvent fractions using hexane, ethylacetate (EA), butanol, and water residue were prepared in an effort at the efficient re-use of the lees of SPS. The ethanol extraction yield was 1.36-fold higher than that of the hot water extraction, and the EA fraction revealed the highest total polyphenol content among the solvent fractions. The various extracts and solvent fractions did not demonstrate hemolytic activity at up to 0.5 mg/ml concentrations against human red blood cells. In the bio-activity assay, only the EA fraction displayed a broad spectrum of anti-microbial activity against different pathogenic and food spoilage bacteria, and demonstrated significant anti-coagulation activity by inhibitions of thrombin, prothrombin and blood coagulation factors. Furthermore, only the EA fraction from the hot water extract of the lees of SPS showed anti-platelet aggregation activity, which is comparable to aspirin (a commercially available drug). Our results suggest that the EA fraction of the hot water extract prepared from the lees of SPS has a high potential as a novel resource for anti-microbial and anti-thrombosis agents. © 2014, The Korean Society for Microbiology and Biotechnology.


Kim M.-S.,Andong National University | Lee Y.-S.,Andong National University | Kim J.S.,Andong National University | Shin W.-C.,Kooksoondang Brewery Co. | Sohn H.-Y.,Andong National University
Korean Journal of Microbiology and Biotechnology | Year: 2014

Ehwa nuruk (EN), a traditional Korean alcoholic rice beverage, is manufactured from pulverized wet rice and the needles of pine trees. In this study, the ethanol extract of EN and its subsequent organic solvent fractions were prepared, and their in-vitro anti-thrombosis activity evaluated. In an anti-coagulation assay, only the ethylacetate (EA) fraction of the ethanol extract showed significant extensions of thrombin time, prothrombin time and activated partial thromboplastin time. In a platelet aggregation assay, the water residue of the ethanol extract exhibited aggregation inhibitory activity. Our results suggest that the EA fraction has potential as a new anticoagulation agent and EN could be used as a novel resource for anti-thrombosis agents. This report provides the first evidence of the anti-thrombosis activity of EN. © 2014, The Korean Society for Microbiology and Biotechnology.


Lee S.-J.,Kooksoondang Brewery Co. | Kwon Y.-Y.,Kooksoondang Brewery Co. | Cho S.-W.,Kooksoondang Brewery Co. | Kwon H.-S.,Kooksoondang Brewery Co. | Shin W.-C.,Kooksoondang Brewery Co.
Journal of the Korean Society of Food Science and Nutrition | Year: 2013

Extrinsic skin aging is characterized by the loss of skin tone and resilience, irregular pigmentation, and deep wrinkles. The aim of this study was to investigate the effects of Ehwa Makgeolli containing oriental herbs (Glycyrrhiza uralensis Fisch., Lycium chinense MILL., Morus alba L., and Saururus chinensis Baill) on skin whitening and wrinkling in human skin cells. We prepared Makgeolli extracts (HEE) with 70% ethanol. HEE significantly inhibited in vitro mushroom tyrosinase activity and reduced the cellular and secreted melanin content of mouse melanoma melanocytes (B16F1 cells). HEE down-regulated the protein expression of tyrosinase related protein (TRP)-1/-2, a key player in melanogenesis. Treatment with HEE in human keratinoctyes (HaCaT cells) inhibited the proteolytic activities of matrix metalloproteinase (MMP)-2/-9 in a dose-dependent manner and dramatically reduced the expression of MMP-2/-9. In addition, HEE attenuated lipopolysaccharide (LPS)- induced nitric oxide production in murine macrophages (RAW264.7 cells). These results indicate that HEE may be a great cosmeceutical ingredient for its whitening, anti-wrinkle, and anti-inflammatory effects.


Kim M.-S.,Andong National University | Lee Y.-S.,Andong National University | Kim J.S.,Andong National University | Shin W.-C.,Kooksoondang Brewery Co. | Sohn H.-Y.,Andong National University
Korean Journal of Microbiology and Biotechnology | Year: 2015

Nuruk has been used as fermentation starter in the alcohol industry for some time in Korea. Various bioactivities, such as antiproliferative and anti-obesity, of R4 nuruk made from Rhizopus oryzae KSD-815 have been previously reported. In this study, the hot water and ethanol extract of R4 nuruk and their subsequent organic solvent fractions were prepared, and their anti- ! thrombosis activities were evaluated. The ethylacetate fraction showed strong anti-coagulation activity, and the ethylacetate j and butanol fraction from hot water extract demonstrated platelet aggregation inhibitory activity, without hemolysis against i human RBC. Our results suggest that R4 nuruk has the potential to act as a new anti-thrombosis agent. © 2015, The Korean Society for Microbiology and Biotechnology.


Lee S.-J.,Kooksoondang Brewery Co. | Cho S.-W.,Kooksoondang Brewery Co. | Kwon Y.-Y.,Kooksoondang Brewery Co. | Kwon H.-S.,Kooksoondang Brewery Co. | Shin W.-C.,Kooksoondang Brewery Co.
Mycobiology | Year: 2012

Nuruk contributes to the unique characteristics of Korean alcoholic beverages. In this study, the effects of nuruk extracts (NE) on anti-oxidant characters, melanogenesis, and anti-photoaging activity were investigated. NEs were obtained from the 70% ethanol extracts of six types of nuruk, which have been used in brewing of fermented alcohol beverages in Korea. First, various antioxidant characteristics were identified in terms of 2,2'-azino-bis(3-ethylbenzthiozoline-6-sulphonic acid) (ABTS) radical scavenging activity, superoxide dismutase (SOD) expression, and inhibition of xanthine oxidase activity. NE#4 exhibited potent ABTS radical scavenging activity (IC50 = 19.51 μg/mL). Compared with NE#4, relatively lower levels of activity were observed for NE#3 and NE#6, with IC50 values of 90.99 and 76.88 μg/mL, respectively. According to results of western blot analysis for determination of SOD expression in H2O2-treated HepG2 cells, NE#5 and NE#6 induced a dramatic increase in the expression ratio of SOD, compared to the group treated with H2O2 only. Activity of xanthine oxidase, which converts xanthine into uric acid, generating superoxide ions, was inhibited by NE#4 and NE#6 in a dose-dependent manner. NE#4 induced significant inhibition of mushroom tyrosinase activity. A reduction in cellular melanin contents of 80% was observed in B16F1 melanocytes treated with NE#5 and NE#6; these effects were similar to those of arbutin at 100 μM. In addition, gelatin zymography and reverse transcription-PCR analysis were performed for assessment of anti-photoaging activity of Nuruk. Treatment with NE#6 resulted in dramatically inhibited activities of matrix metalloproteinase (MMP)-2/-9, suppressed expression of MMP-1, and increased expression of type-1 procollagen. Results of gelatin zymography for NE#4 and NE#5 were similar, to a slightly lesser degree. These results suggest the potential of NE#4 and NE#6 as natural ingredients for use in functional foods and cosmetics. © The Korean Society of Mycology.


Kim M.S.,Andong National University | Shin W.C.,Kooksoondang Brewery Co | Kang D.K.,Gyeongsangbukdo Agricultural Research and Extension Services | Sohn H.Y.,Andong National University
Journal of Microbiology and Biotechnology | Year: 2016

From the lees of bokbunja wine (LBW) made from Rubus coreanus Miquel, we have identified six compounds (1: trans-4-hydroxycinnamic acid; 2: trans-4-hydroxy-3-methoxycinnamic acid; 3: 3,4-dihydroxycinnamic acid; 4: 4-hydroxy-3-methoxybenzoic acid; 5: 3,5-dimethoxy-4- hydroxybenzoic acid; and 6: 3,5-dimethoxy-4-hydroxycinnamic acid (sinapic acid)) through silica gel chromatography and UHPLC-MS. The compounds 1-6 showed strong anticoagulation and platelet aggregation inhibitory activities without hemolytic effect against human red blood cells. To date, this is the first report of the in vitro anti-thrombosis activity of sinapic acid. Our results suggest that different cinnamic and benzoic acid derivatives are closely linked to the anti-thrombosis activity of LBW, and sinapic acid could be developed as a promising anti-thrombosis agent. © 2016 by The Korean Society for Microbiology and Biotechnology.

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