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Gyeonggi, South Korea

Kim M.-S.,Andong National University | Lee Y.-S.,Andong National University | Kim J.S.,Andong National University | Shin W.-C.,Kooksoondang Brewery Co. | Sohn H.-Y.,Andong National University
Korean Journal of Microbiology and Biotechnology | Year: 2014

Sweet potato soju (SPS), a form of traditional distilled alcoholic liquor in Korea, is manufactured by the distillation of fermented broth under normal pressure, thus providing it for a uniquely smooth taste infused with the flavor of sweet potato. After distillation, the lees of SPS is produced as by-product and discarded. In this study, the ethanol and hot water extracts of lees of SPS, and their subsequent organic solvent fractions using hexane, ethylacetate (EA), butanol, and water residue were prepared in an effort at the efficient re-use of the lees of SPS. The ethanol extraction yield was 1.36-fold higher than that of the hot water extraction, and the EA fraction revealed the highest total polyphenol content among the solvent fractions. The various extracts and solvent fractions did not demonstrate hemolytic activity at up to 0.5 mg/ml concentrations against human red blood cells. In the bio-activity assay, only the EA fraction displayed a broad spectrum of anti-microbial activity against different pathogenic and food spoilage bacteria, and demonstrated significant anti-coagulation activity by inhibitions of thrombin, prothrombin and blood coagulation factors. Furthermore, only the EA fraction from the hot water extract of the lees of SPS showed anti-platelet aggregation activity, which is comparable to aspirin (a commercially available drug). Our results suggest that the EA fraction of the hot water extract prepared from the lees of SPS has a high potential as a novel resource for anti-microbial and anti-thrombosis agents. © 2014, The Korean Society for Microbiology and Biotechnology. Source


Kim M.-S.,Andong National University | Lee Y.-S.,Andong National University | Kim J.S.,Andong National University | Shin W.-C.,Kooksoondang Brewery Co. | Sohn H.-Y.,Andong National University
Korean Journal of Microbiology and Biotechnology | Year: 2014

Ehwa nuruk (EN), a traditional Korean alcoholic rice beverage, is manufactured from pulverized wet rice and the needles of pine trees. In this study, the ethanol extract of EN and its subsequent organic solvent fractions were prepared, and their in-vitro anti-thrombosis activity evaluated. In an anti-coagulation assay, only the ethylacetate (EA) fraction of the ethanol extract showed significant extensions of thrombin time, prothrombin time and activated partial thromboplastin time. In a platelet aggregation assay, the water residue of the ethanol extract exhibited aggregation inhibitory activity. Our results suggest that the EA fraction has potential as a new anticoagulation agent and EN could be used as a novel resource for anti-thrombosis agents. This report provides the first evidence of the anti-thrombosis activity of EN. © 2014, The Korean Society for Microbiology and Biotechnology. Source


Kim M.-S.,Andong National University | Lee Y.-S.,Andong National University | Kim J.S.,Andong National University | Shin W.-C.,Kooksoondang Brewery Co. | Sohn H.-Y.,Andong National University
Korean Journal of Microbiology and Biotechnology | Year: 2015

Nuruk has been used as fermentation starter in the alcohol industry for some time in Korea. Various bioactivities, such as antiproliferative and anti-obesity, of R4 nuruk made from Rhizopus oryzae KSD-815 have been previously reported. In this study, the hot water and ethanol extract of R4 nuruk and their subsequent organic solvent fractions were prepared, and their anti- ! thrombosis activities were evaluated. The ethylacetate fraction showed strong anti-coagulation activity, and the ethylacetate j and butanol fraction from hot water extract demonstrated platelet aggregation inhibitory activity, without hemolysis against i human RBC. Our results suggest that R4 nuruk has the potential to act as a new anti-thrombosis agent. © 2015, The Korean Society for Microbiology and Biotechnology. Source


Kim M.S.,Andong National University | Shin W.C.,Kooksoondang Brewery Co. | Kang D.K.,Gyeongsangbukdo Agricultural Research and Extension Services | Sohn H.Y.,Andong National University
Journal of Microbiology and Biotechnology | Year: 2016

From the lees of bokbunja wine (LBW) made from Rubus coreanus Miquel, we have identified six compounds (1: trans-4-hydroxycinnamic acid; 2: trans-4-hydroxy-3-methoxycinnamic acid; 3: 3,4-dihydroxycinnamic acid; 4: 4-hydroxy-3-methoxybenzoic acid; 5: 3,5-dimethoxy-4- hydroxybenzoic acid; and 6: 3,5-dimethoxy-4-hydroxycinnamic acid (sinapic acid)) through silica gel chromatography and UHPLC-MS. The compounds 1-6 showed strong anticoagulation and platelet aggregation inhibitory activities without hemolytic effect against human red blood cells. To date, this is the first report of the in vitro anti-thrombosis activity of sinapic acid. Our results suggest that different cinnamic and benzoic acid derivatives are closely linked to the anti-thrombosis activity of LBW, and sinapic acid could be developed as a promising anti-thrombosis agent. © 2016 by The Korean Society for Microbiology and Biotechnology. Source


Jung S.K.,Seoul National University | Lim T.G.,Konkuk University | Kim J.E.,Seoul National University | Byun S.,Seoul National University | And 6 more authors.
Food Chemistry | Year: 2012

Consumption of fruits and vegetables is correlated with a lower incidence of cancer. Here, we identified hyperoside as an active compound from Acanthopanax sessiliflorus, and investigated the effect of hyperoside on UVB-induced transactivation of activator protein 1 (AP-1) and on the mitogen-activated protein kinase signalling pathway in JB6 P + cells. Hyperoside inhibited UVB-induced AP-1 transactivation. It inhibited the UVB-induced phosphorylation of p90RSK. Kinase assays revealed that hyperoside significantly inhibited ERK1/2 activity. Furthermore, hyperoside bound to ERK1/2 to suppress its activity. In addition, phosphorylation of cAMP response element binding protein (CREB) and signal transducers and activators of transcription (STAT) 3 were suppressed by hyperoside. Overall, these results indicate that hyperoside may be a promising chemopreventive agent that acts by suppressing the transactivation of AP-1 and the phosphorylation of p90RSK, CREB, and STAT3 through the binding and inhibition of ERK1/2. © 2011 Elsevier Ltd. All rights reserved. Source

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