Kolon Life Science Research Institute

Gyeonggi Do, South Korea

Kolon Life Science Research Institute

Gyeonggi Do, South Korea
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Park S.-H.,Pohang University of Science and Technology | Doh J.,Pohang University of Science and Technology | Park S.I.,Catholic University of Korea | Lim J.Y.,Catholic University of Korea | And 20 more authors.
Gene Therapy | Year: 2010

Cell-permeable peptides (CPPs) promote the transduction of nonpermissive cells by recombinant adenovirus (rAd) to improve the therapeutic efficacy of rAd. In this study, branched oligomerization of CPPs significantly enhanced the transduction of human mesenchymal stem cells (MSCs) by rAd in a CPP type-independent manner. In particular, tetrameric CPPs increased transduction efficiency at 3000-5000-fold lower concentrations than did monomeric CPPs. Although branched oligomerization of CPPs also increases cytotoxicity, optimal concentrations of tetrameric CPPs required for maximum transduction are at least 300-1000-fold lower than those causing 50% cytotoxicity. Furthermore, although only ∼60% of MSCs were maximally transduced at 500 M of monomeric CPPs, >95% of MSCs were transduced with 0.1 M of tetrameric CPPs. Tetrameric CPPs also significantly increased the formation and net surface charge of CPP/rAd complexes, as well as the binding of rAd to cell membranes at a greater degree than did monomeric CPPs, followed by rapid internalization into MSCs. In a critical-size calvarial defect model, the inclusion of tetrameric CPPs in ex vivo transduction of rAd expressing bone morphogenetic protein 2 into MSCs promoted highly mineralized bone formation. In addition, MSCs that were transduced with rAd expressing brain-derived neurotrophic factor in the presence of tetrameric CPPs improved functional recovery in a spinal cord injury model. These results demonstrated the potential for tetrameric CPPs to provide an innovative tool for MSC-based gene therapy and for in vitro gene delivery to MSCs. © 2010 Macmillan Publishers Limited All rights reserved.

Nguyen H.M.,Korea University | Hwang I.C.,Central Research Institute | Kweon D.K.,Kolon Life Science Research Institute | Park H.J.,Korea University | Park H.J.,Clemson University
Journal of Microencapsulation | Year: 2013

With the aim to effectively enhance the payload for nanocarriers, supersaturated deltamethrin (SSD) solution was prepared using the solvent-mediated method to produce lipid nanocarriers by a combination method of homogenization and sonication. In this study, deltamethrin was used as an active ingredient, corn oil was used as a lipid medium, soybean lecithin and Tween-80 were used as surfactants. At 25°C, the solubility of deltamethrin in SSD solution prepared by solvent-mediated method was 3.4 times and 1.5 times higher than that in saturated deltamethrin (SD) solution and that in SSD solution prepared by thermal-mediated method, respectively. Therefore, compared to the use of SD solution for the production of nanocarriers, the use of SSD solution significantly enhanced the payload, while keeping the encapsulation efficiency high. Particularly, nanocarriers produced from SSD solution prepared by solvent-mediated method had the higher payload of 2.1 times and 4.4 times compared with preparations using SSD solution prepared by thermal-mediated method and SD solution, respectively. © 2013 Informa UK Ltd. All rights reserved.

Kong M.,Korea University | Chen X.G.,Ocean University of China | Kweon D.K.,Kolon Life Science Research Institute | Park H.J.,Korea University
Carbohydrate Polymers | Year: 2011

An alcohol-free oil/water hyaluronic acid nanoemulsion was developed to be applied as transdermal carrier for active lipophilic ingredient. In vitro hemolysis, skin penetration and histological examinations were carried out using α-tocopherol as model ingredient to assess skin permeability and bioavailability. Without any chemical enhancers, nanoemuslion performed desirable skin permeable capacity, being able to penetrate across stratum corneum and diffuse deeper into dermis compared with the control group (ethanol solution) via follicular and intercellular pathway. Penetration mechanism was preliminarily studied and suggested to be closely concerned with transmembrane concentration gradient, carrier characteristics and penetration enhancers. No irritation has been found in dermis and skin surface indicated hyaluronic acid nanoemulsions could be successfully used as purcutaneous delivery carrier of active lipophilic ingredient and favorable for drug and cosmetic applications. © 2011 Elsevier Ltd All rights reserved.

Jung D.-H.,Kyung Hee University | Jung J.-H.,Kyung Hee University | Seo D.-H.,Kyung Hee University | Ha S.-J.,Kangwon National University | And 2 more authors.
Bioresource Technology | Year: 2013

Amylosucrase (AS), maltooligosyltrehalose synthase (MTS), and maltooligosyltrehalose trehalohydrolase (MTH) were used in combined cross-linked enzyme aggregates (combi-CLEAs) to achieve one-step bioconversion of sucrose to trehalose. Combi-CLEAs of three enzymes were successfully established with acetone and glutaraldehyde (GA) as a precipitant and a cross-linker, respectively. The optimum enzyme ratio was 8:0.5:0.5 (AS, 4. mg:MTS, 0.25. mg:MTH, 0.25. mg). To improve trehalose production, bovine serum albumin was co-aggregated with enzymes as a proteic feeder. The trehalose production yield of combi-CLEAs was about 8% in each cycle on the basis of substrate added up to 400. mM. Finally, the combi-CLEAs used in this experiment showed reusability of about five cycles without any activity loss. © 2013 Elsevier Ltd.

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