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Gurav U.,Kolhapur Institute of Technology
ICWET 2010 - International Conference and Workshop on Emerging Trends in Technology 2010, Conference Proceedings | Year: 2010

Over the past few years, researchers have been striving for a Utility Computing Model. Cloud computing allows delivering information technology on demand. Cloud Computing service providers provides resources by means of virtualization. Applying these techniques however goes along with handling over ultimate control of data to a third party. This paper discusses several issues in virtualization security, summery of various solutions, risk prevention in VMM (virtual machine monitor). Here an attempt is made to perform the analysis of various issues pertaining to virtualization and security. Copyright 2010 ACM.


Durgawale P.,Krishna Institute of Medical science | Sontakke A.,Krishna Institute of Medical science | Shukla P.S.,Manipal Institute Medical science | Durgawale P.,Kolhapur Institute of Technology | Chougule P.,Krishna Institute of Medical science
Biomedical Research | Year: 2011

Total serum sialic acid (TSA), total proteins (TP) and TSA/TP ratio (as tumor markers) have been estimated in 108 cancer patients, 30 pathological controls and 50 normal healthy controls. Data analysis indicated a significant increase (p< 0.001) in the TSA and TSA/TP ratio in cancer patients (98.41 ± 13.5 mg/dl and 15.31 ± 2.57 mg/gm) as compared to pathological controls (77.57 ± 21.09 mg/dl and 11.94 ± 3.43 mg/gm) and normal control (64.77 ± 7.98 mg/dl and 8.9 ± 1.15mg/gm) respectively. A significant decrease (p<0.001) in serum levels of total proteins were found in all cases of cancer compared to the normal (6.46 ± 0.48 mg/dl and 7.29 ± 0.55 gm/dl) and pathological controls (6.53 ± 0.36 gm/dl).Further analysis of the data in patient subgroups of malignancy based on tissue involved indicated that the lack of specificity of the markers but the sensitivity of TSA and TSA/TP increased as the malignancy become more severe. The results show that TSA/TP was the most useful marker for detecting malignancies.


Talekar S.,Kolhapur Institute of Technology | Joshi A.,Kolhapur Institute of Technology | Chougle R.,Kolhapur Institute of Technology | Nakhe A.,Kolhapur Institute of Technology | Bhojwani R.,Kolhapur Institute of Technology
Nano-Structures and Nano-Objects | Year: 2016

NADH-dependent nitrate reductase CLEAs catalyzed synthesis of silver nanoparticles from silver nitrate has been investigated. NADH-dependent nitrate reductase CLEAs catalyzed reduction of silver ions to silver nanoparticles at pH 7.2 using NADH as electron source and 8-hydroxyquinoline as electron shuttle. Fourier transform infrared spectroscopy (FTIR) and Energy dispersive spectroscopy (EDS) showed that 8-hydroxyquinoline also acts as capping agent of silver nanoparticles. The effects of reaction time, reaction temperature, silver nitrate concentration and amount of CLEAs on silver nanoparticles synthesis were studied with aid of UV-Vis spectrophotometer. Optimum silver nanoparticles synthesis occurred after 24 h reaction of 100 mg CLEAs with 1 mM silver nitrate at pH 7.2 and temperature 35 °C. Scanning electron microscopy (SEM), Transmission electron microscopy (TEM) and High resolution transmission electron microscopy (HRTEM) showed the silver nanoparticles were spherical in shape. The particle size calculated using Dynamic light scattering measurements (DLS) was found to be in the range of 5-7 nm. X-ray diffraction (XRD) and selected area electron diffraction (SAED) patterns confirmed formation of crystalline silver nanoparticles with face-centred-cubic (fcc) lattice structure. Notably, nitrate reductase CLEAs showed 80% catalytic yield even after 5 cycles of repeated use in silver nanoparticle synthesis at pH 7.2 and temperature 35 °C. Moreover, silver nanoparticles of same morphology and dimensions were formed in each cycle. © 2016 Elsevier B.V. All rights reserved.


Talekar S.,Kolhapur Institute of Technology | Joshi G.,Kolhapur Institute of Technology | Chougle R.,Kolhapur Institute of Technology | Nainegali B.,Kolhapur Institute of Technology | And 7 more authors.
Catalysis Communications | Year: 2014

The NADH-dependent nitrate reductase from Fusarium oxysporum cell extract was directly immobilized as cross linked enzyme aggregates (CLEAs) and investigated for the synthesis of silver nanoparticles by a reduction of silver nitrate. The effects of precipitant type and cross-linking on activity recovery of enzyme in CLEAs were studied. After aggregation of enzyme with ammonium sulfate followed by cross-linking formed aggregates for 4 h with 8 mM glutaraldehyde, 93% activity recovery was achieved in CLEAs with enhanced thermal stability at 50 °C and 40°C. Scanning electron microscopy analysis showed that immobilized NADH-dependent nitrate reductase was of spherical structure. CLEAs showed 90% catalytic yield even after 4 cycles of repeated use in silver nanoparticle synthesis at pH 7.2 and temperature 35 °C. © 2014 Elsevier B.V.


Koli G.C.,Shivaji University | Kulkarni V.V.,Kolhapur Institute of Technology
WCE 2010 - World Congress on Engineering 2010 | Year: 2010

Sloshing has widespread applications in many industries including automotive, aerospace, ship building and motorcycle manufacturing. The goal of sloshing simulation is to first study the sloshing pattern and then improve the tank design to reduce noise levels, stresses on the structure and optimize the baffle arrangements. In this project simulation of the fluid in tank is studied and the design modification with baffle plate is considered to minimize the sloshing phenomena using ALE method. Also it is explained that there is need to analyze the sloshing phenomena in detail. Arbitrary Langrangian Eulerian finite element methods gain interest for the capability to control mesh geometry independently from material geometry, the ALE methods are used to create a new undistorted mesh for the fluid domain. In this paper we use the ALE technique to solve fuel slosh problem. Fuel slosh is an important design consideration not only for the fuel tank, but also for the structure supporting the fuel tank. Fuel slosh can be generated by many ways: abrupt changes in acceleration (braking), as well as abrupt changes in direction (highway exit-ramp). Repetitive motion can also be involved if a sloshing resonance is generated. These sloshing events can in turn affect the overall performance of the parent structure. A finite element analysis method has been developed to analyze this complex event. A new ALE formulation for the fluid mesh can be used to keep the fluid mesh integrity during the motion of the tank. This paper explains the analysis capabilities on a technical level.


Talekar S.,Kolhapur Institute of Technology | Nadar S.,Kolhapur Institute of Technology | Joshi A.,Kolhapur Institute of Technology | Joshi G.,Kolhapur Institute of Technology
RSC Advances | Year: 2014

Pectin cross-linked enzyme aggregates (pectin-CLEAs) of glucoamylase were prepared for the first time with pectin as cross-linking agent. Pectin as a biocompatible, biodegradable, non-toxic, renewable and macromolecular cross-linker was used instead of traditional micro-molecular glutaraldehyde cross-linker. The cross-linker was prepared by controlled sodium metaperiodate oxidation of native pectin. The effects of precipitant type, amount of precipitant and cross-linking on activity recovery of glucoamylase in pectin-CLEAs were studied. After aggregation of glucoamylase with ammonium sulphate, when formed aggregates were cross-linked by pectin, 83% activity recovery was achieved in pectin-CLEAs, whereas when cross-linked by traditional cross-linker glutaraldehyde, 64% activity recovery was achieved in glutaraldehyde-CLEAs. After formation of pectin-CLEAs and glutaraldehyde-CLEAs, the optimum temperature for glucoamylase activity was shifted from 50 to 55 °C. The free enzyme and pectin-CLEAs displayed an optimal pH of 5, whereas the optimal pH of glutaraldehyde-CLEAs was shifted to pH 6. Compared with the free enzyme and glutaraldehyde-CLEAs, lower inactivation rate constant of glucoamylase in pectin-CLEAs within the temperature range of 50-70°C was observed. Moreover, the activation energy required for denaturation of glucoamylase in pectin-CLEAs was higher than glutaraldehyde-CLEAs and free enzyme. Kinetic studies show that the Km and Vmax of glucoamylase remained unchanged after pectin-CLEAs formation, whereas Km was increased and Vmax was decreased after glutaraldehyde-CLEAs formation. Finally upon 10 consecutive uses, pectin-CLEAs retained 55% initial activity and glutaraldehyde-CLEAs retained only 29% initial activity. These results suggest that this pectin-CLEA is potentially usable in industrial applications. © The Royal Society of Chemistry.


Talekar S.,Kolhapur Institute of Technology | Shah V.,Kolhapur Institute of Technology | Patil S.,Kolhapur Institute of Technology | Nimbalkar M.,Kolhapur Institute of Technology
Catalysis Science and Technology | Year: 2012

In this work, porous cross-linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase are reported for the first time. Porous CLEAs were prepared by adding starch as a pore-making agent in crude invertase solution and co-precipitating with invertase using ammonium sulfate as a precipitating agent. The resultant co-precipitate was cross-linked for 8 h with 20 mM glutaraldehyde and in the end starch was removed by alpha amylase. The highest catalytic efficiency was obtained at 0.4% (w/v) starch. The scanning electron microscopy analysis of invertase p-CLEAs showed a porous structure. The p-CLEAs were shown to have improved internal mass transfer compared to conventional CLEAs. © 2012 The Royal Society of Chemistry.


Dhamole P.B.,Birla Institute of Technology and Science | Demanna D.,Kolhapur Institute of Technology | Desai S.A.,Kolhapur Institute of Technology
Applied Biochemistry and Biotechnology | Year: 2014

Ferulic acid (FA) and p-coumaric acid (pCA) are high-value products that can be obtained by alkaline hydrolysis of lignocellulose. Present work explores the potential of surfactant-based cloud-point extraction (CPE) for FA and pCA extraction from corn cob hydrolysate. More than 90 % (w/w) extraction of both FA and pCA was achieved from model system with L92. The partition coefficient of FA and pCA in L92 aqueous phase system was 35 and 55, respectively. A significant enrichment (8-10-fold) of both FA and pCA was achieved in surfactant-rich phase. Furthermore, the downstream process volume was reduced by 10 to 13 times. Optimized conditions (5 %v/vL92 and pH 3.0) resulted into 85 and 89 % extraction of FA and p-CA, respectively, from alkaline corn cob hydrolysate. Biocompatibility tests were carried out for L92 for ethanol fermentation and found to be biocompatible. Thus, the new surfactant-based CPE system not only concentrated FA and pCA but also reduced the process volume significantly. Further, aqueous phase containing sugars can be used for ethanol fermentation. © 2014 Springer Science+Business Media.


Talekar S.,Kolhapur Institute of Technology | Joshi A.,Kolhapur Institute of Technology | Joshi G.,Kolhapur Institute of Technology | Kamat P.,Kolhapur Institute of Technology | And 2 more authors.
RSC Advances | Year: 2013

In the past couple of decades, cross linked enzyme aggregates (CLEAs) have emerged as a novel and versatile carrier free immobilization technique. The immobilization of enzymes as cross linked enzyme aggregates (CLEAs) involves precipitation of an enzyme from aqueous solution followed by cross linking with a bi-functional reagent. It is worth noting that many parameters alter the enzyme precipitation and the aggregate cross linking and hence affect the activity and stability of CLEAs. Therefore to endorse CLEAs for industrial application, each newly synthesised CLEA is characterized. This review intends to investigate the effects of various parameters, such as the nature and purity of the enzyme, the nature and amount of precipitant, the nature and amount of cross linker, the cross linking time, the pH and temperature during CLEA preparation and washing and separation techniques on the activity and stability of CLEAs. The major parameters such as catalytic properties, particle size and morphology, stability and reusability required for approval of industrial applicability of newly synthesized CLEAs are critically reviewed. Furthermore the scope of CLEAs in non-aqueous solvent, the development of one pot cascade processes and the design of different types of enzyme reactors is also discussed. © The Royal Society of Chemistry 2013.

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