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Tambe Y.,Shiga University of Medical Science | Hasebe M.,Shiga University of Medical Science | Hasebe M.,Bioscience Technology | Kim C.J.,Kohka Public Hospital | And 2 more authors.
Molecular Carcinogenesis | Year: 2016

Previously, we showed that drs contributes to suppression of malignant tumor formation in drs-knockout (KO) mice. In this study, we demonstrate the regulation of glucose metabolism by drs using comparisons of drs-KO and wild-type (WT) mouse embryonic fibroblasts (MEFs). Extracellular acidification, lactate concentration, and glucose consumption in drs-KO cells were significantly greater than those in WT cells. Metabolomic analyses also confirmed enhanced glycolysis in drs-KO cells. Among glycolysis-regulating proteins, expression of lactate dehydrogenase (LDH)-B was upregulated at the post-transcriptional level in drs-KO cells and increased LDH-B expression, LDH activity, and acidification of culture medium in drs-KO cells were suppressed by retroviral rescue of drs, indicating that LDH-B plays a critical role for glycolysis regulation mediated by drs. In WT cells transformed by activated K-ras, expression of endogenous drs mRNA was markedly suppressed and LDH-B expression was increased. In human cancer cell lines with low drs expression, LDH-B expression was increased. Database analyses also showed the correlation between downregulation of drs and upregulation of LDH-B in human colorectal cancer and lung adenocarcinoma tissues. Furthermore, an LDH inhibitor suppressed anchorage-independent growth of human cancer cells and MEF cells transformed by activated K-ras. These results indicate that drs regulates glucose metabolism via LDH-B. Downregulating drs may contribute to the Warburg effect, which is closely associated with malignant progression of cancer cells. © 2016 Wiley Periodicals, Inc.

Okino T.,Kohka Public Hospital
Gan to kagaku ryoho. Cancer & chemotherapy | Year: 2012

Kohka Public Hospital(KPH), approved as a regional cancer base hospital in February 2008, has concentrated on cancer treatment and care. KPH developed a Division of Home Care Medicine last year. A home care doctor visited and treated various patients, including cancer patients by co-operating with the Visiting Nurse Station; this resulted in an increase in home care patients but not of home deaths. To increase the cases of home care medicine, we believe that collaboration with other institutes and the creation of a regional network conference consisting of doctors, nurses, pharmacists, dental doctors, home helpers, and regional administrators is necessary. Via the conference, we recognized the need to obtain more information and create a system of collaborative home medication and care for cancer patients.

Okino T.,Kohka Public Hospital
Gan to kagaku ryoho. Cancer & chemotherapy | Year: 2011

Kohka Public Hospital(KPH), located in the southeast district of Shiga Prefecture, has been recognized for playing a major role in the care of acute physical disorders. KPH is also expected to have a central role in cancer treatment in the regional medical district. We have practiced home care on terminal cancer patients since before the establishment of palliative care team at KPH in 2008. The Visiting Nurse Station treated a total of 82 terminal cancer patients from 2008 through 2010 fiscal year. Of these 23 patients we cared for died at home. On the other hand, the Division of Regional Medical Collaboration carried out 23-home visit medication for last 2 years(2009-2010). Of these 23-home visits, they cared for 7 patients who died at home. The majority of the patients, as well as families, wished that they would have liked to be re-hospitalized at KPH in their terminal stage. Under the consideration of these results, we established a Division of Home Care Medicine this year and would intend to cooperate and to take care of the cancer patients not only at KPH, but also at various other places in cooperation with the regional medical resources and welfare after creating a medical network system for cancer treatment and care.

Kim C.J.,Kohka Public Hospital | Tambe Y.,Setatsukinowa cho | Mukaisho K.-I.,Setatsukinowa cho | Sugihara H.,Setatsukinowa cho | And 5 more authors.
Carcinogenesis | Year: 2014

Human cyclin D1 generates two major isoforms via alternative splicing: cyclin D1a and cyclin D1b. Cyclin D1b is hardly expressed in normal tissues but is frequently expressed in certain types of cancer tissues. To clarify the oncogenic potential of cyclin D1b variant, we developed cyclin D1b transgenic (Tg) mice and analyzed their phenotypes. We detected rectal tumors in 63% (15/24) of the female Tg mice. All rectal tumors had the histological characteristics similar to human sessile serrated adenoma/ polyps (SSA/Ps). Adenocarcinomas were also found in 53% (8/15) of the rectal tumors, suggesting that these adenocarcinomas originated from the SSA/P-like lesions. No rectal tumors were found in the ovariectomized female cyclin D1b Tg mice (0/10), indicating that ovarian hormones played a critical role in rectal carcinogenesis in these Tg mice. Both phosphorylation of Erk, without activating MEK, and expression of estrogen receptor β were elevated in the rectal tumors of female cyclin D1b Tg mice compared with normal rectums of female wild-type mice. In addition, we established a cell line, D1bTgRT, derived from a rectal cancer of female Tg mouse. Small interfering RNA-induced cyclin D1b knockdown in this cell line suppressed Erk phosphorylation, anchorageindependent growth, cell invasiveness and tumorigenicity in nude mice. In humans, expression of cyclin D1b messenger RNA was detected in 17% (1/6) of colorectal cancer cell lines and 9.7% (3/31) of colorectal cancer tissues. Taken together, these results indicate that cyclin D1b expression contributes to the femalespecific rectal carcinogenesis in mouse model. © The Author 2013. Published by Oxford University Press. All rights reserved.

Kawaguchi Y.,Kohka Public Hospital | Fujita T.,Kohka Public Hospital | Hanaoka J.,Shiga University of Medical Science
Annals of Thoracic Surgery | Year: 2015

Desmoplastic fibroblastoma is a rare soft tissue tumor. We present the case of a 61-year-old man with a growing chest wall tumor in the pleural cavity. After resection, the tumor was diagnosed as desmoplastic fibroblastoma. This is the first report of desmoplastic fibroblastoma occurring in the chest wall. Generally, desmoplastic fibroblastoma is considered as a benign and slow-growing tumor. However, the tumor in our patient grew very rapidly and invaded the adjacent lung, parietal pleura, and periosteum of the rib bone. We therefore describe this tumor as an "invasive" desmoplastic fibroblastoma. © 2015 The Society of Thoracic Surgeons.

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