Sultānpur, India
Sultānpur, India

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Singh J.,T D College | Singh A.L.,KNIPSS | Das V.K.,KNIPSS | Singh P.B.,Ganpat Sahai Postgraduate College
Journal of Ecophysiology and Occupational Health | Year: 2011

The aim of the present investigation was to monitor the levels of bioaccumulation of carbamates (Carbofuran, Carbaryl and Aldicarb) and pyrethroid insecticides (Cypermethrin and Deltamethrin) in liver, brain and ovary of captured catfish Bagarius bagarius from unpolluted ponds of Gujartal, Jaunpur (reference site) and polluted river Gomti, Jaunpur during pre-monsoon or breeding phase. The carbamates were measured by high performance liquid chromatography (HPLC) and the pyrethroids by gas liquid chromatography (GLC). Results indicated that catfish B. bagarius captured from river Gomti have higher bioaccumulation of insecticides when compared to those from the reference site reflecting the degree of pesticide pollution present in those water bodies. The bioaccumulation of Carbofuran, Carbaryl and Aldicarb were significantly higher in the brain, liver and ovary in polluted fish as compared to the conspecific species captured from reference site. Likewise, the bioaccumulation of Cypermethrin and Deltamethrin was also higher in the studied tissues when compared with fish of same species captured from the reference site. Results indicated that the bioaccumulation of these insecticides cause metabolic disorder as well as affect the reproductive physiology of this species. © 2011 The Academy of Environmental Biology, India.


Singh A.V.,University of Milan | Aditi A.S.,University of Pune | Gade W.N.,University of Pune | Vats T.,KNIPSS | And 3 more authors.
Current Nanoscience | Year: 2010

Wound healing and ulcer management is a vast topic that stimulated clinical research due to its major socio economic impact on clinical practices. Last decade of research in clinical science has seen materials at microscale developments which significantly contributed to ulcer management and wound healing with their merits and limitations. The present review outlines the new materials at nanoscale that have been designed and manufactured to address the existing problems in wound care but comparatively less familiar to clinicians for tissue repair and ulcer management. Development in tissue engineering practices spanning from micro-to nano dimension provided superior materials over traditional wound dressing materials mimicking tissue in vivo. Recent advances in material sciences and polymer chemistry have yielded an entire class of new nanomaterials, ranging from bioactive tissue scaffold to novel electrospun polymer and hydrogel for tissue repair and wound management. Nanoscale materials with biodegradable chemistries and molecular compositions assist wound healing as adhesive and bandages in specialized wound care. Moreover, new class of materials gives opportunity to engineer tissue substrate in vitro to support bio-responsive nanomedicine for wound healing. Soft nanomaterial design technology involving hydrogel and bio-scaffold revolutionized the wound management supplementing biophysical and biochemical considerations of tissue regeneration. In addition, this review addresses the immediate challenges and opportunities provided by nanoscale tissue engineering in designing synthetic biomaterials as instructive extracellular matrix (ECM) for tissue remodeling and morphogenesis. © 2010 Bentham Science Publishers Ltd.


Sinha D.K.,P.A. College | Singh R.,K.N.I.P.S.S. | Rastogi G.K.,Sunderdeep Engineering College
Indian Journal of Environmental Protection | Year: 2011

Ten different underground drinlcing water samples were collected during the year 2009 from different India Mark 2 handpumps of extensively used different public places at Sultanpur following standards methods of sampling. Samples were quantitatively analysed for a number of physico-chemical parameters by standard methods and procedures prescribed by APHA and estimated values were compared with WHO drinking water standards. The water of almost all the sites was found to be alkaline, hard or very hard and having high extent of chemical contaminants. The drinking water is deficient of essential micro-nutrients and fluoride, an essential element. People exposed to water of study area must be facing the repercussions of polluted drinking water. Water quality management of underground resources at Sultanpur is urgently needed. Present study may assist local authorities in making future underground water quality management policies. © 2011 - Kalpana Corporation.


Prabhuji S.K.,Biotechnology and Molecular Biology Center | Gaur R.,Awadh University | Tripathi A.,KNIPSS | Srivastava G.K.,Biotechnology and Molecular Biology Center | Srivastava R.,Biotechnology and Molecular Biology Center
Medicinal Plants | Year: 2013

Antifungal activity of Saccharomyces cerevisiae culture, isolated from the toddy of Borassus flabellifer, has been studied in vitro on fish pathogenic watermoulds, viz., Achlya orion, Saprolegnia diclina, Aphanomyces laevis and Allomyces arbuscula. Poison-culture method has been used during the experimental procedure. The results have indicated that the effect of Sachharomyces cerevisiae culture was fungistatic and not fungicidal.


Singh R.S.,Gorakhpur University | Singh S.P.,K.N.I.P.S.S. | Gupta G.K.,Gorakhpur University
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2011

Cahill-Glauber C(s)-correspondence is employed to construct Quasi-Probability Distribution Functions (QPDFs) for optical-polarization in phase space following equivalent description of polarization in Classical Optics. The proposed scheme provides pragmatic insights as compared to obscure SU (2) quasi-distributions on Poincare sphere. QPDF (Wigner function) of bi-modal quadrature coherent states is evaluated and numerically investigated to demonstrate the application. © 2011 SPIE.


Nigam S.K.,Ganpat Sahai Postgraduate College | Singh J.,Td College | Singh A.L.,Ganpat Sahai Postgraduate College | Das V.K.,KNIPSS | Singh P.B.,Ganpat Sahai Postgraduate College
Journal of Ecophysiology and Occupational Health | Year: 2011

The present investigation was performed to monitor the HCHs (HCH isomers), DDTs (DDT and its metabolites), chlorpyrifos, plasma levels of estradiol-17β (E2) and gonadosomatic index (GSI) between the sampling sites of the non-polluted ponds of Gujartal, Jaunpur (reference site) and the polluted river Gomti (Jaunpur), affecting the reproductive physiology of some edible female catfish during pre-monsoon season. The HCHs, DDTs and chlorpyrifos were measured by gas liquid chromatography (GLC) and hormone by Enzyme-Linked Immunosorbent Assay (ELISA). The results indicated that the presence of HCHs, DDTs and chlorpyrifos was very high in the catfish captured from polluted river as compared from the conspecific fish captured from reference site. The GSI and E2 declined in female catfish of polluted site when compared to same species from reference site. Results have also indicated that catfish contained high bioaccumulation of HCHs, DDTs and chlorpyrifos which was beyond permissible limit as compared to the female catfish of the reference site. In conclusion, the fishes of river Gomti were highly polluted when compared with the fish of reference site as was evident from high levels of tissue bioaccumulation of HCHs, DDTs, chlorpyrifos and decreased levels of plasma E2 inhibiting the reproductive physiology of these species at receptor level. However, the levels exceeded maximum residue limits (MRL) as recommended by Codex, hence it is suggested that the fish containing pesticide residues beyond permissible limit should be avoided for the food purposes.


Yadav B.B.,Allahabad University | Singh S.K.,Banaras Hindu University | Dubey N.K.,Banaras Hindu University | Shukla S.K.,KNIPSS | Srivastava G.K.,Allahabad University
Taiwania | Year: 2015

In the present study soil, water and plant samples were collected from seven different natural populations of Isoetes dixitei which are found growing on the tablelands of Satara and Kolhapur districts of Maharashtra, India. The collected soil and water samples have been analysed for the colour, texture, available plant nutrients, electrical conductivity, pH and BOD. The soil and water factors along with biotic factors are compared with the number of plants per m2, length of plants, number of leaves and roots per plant to understand the morphological diversity and biology within different populations of I. dixitei. The results of present analysis reveals that these ecological factors such as organic carbon, iron, phosphorus and potassium in soil and low pH of soil and water have positive relationships with the growth, robustness and diversity of I. dixitei. However, copper, zinc and electrical conductivity in the soil have negative significant role in influencing the plant characters. The role of manganese appears to be not significant. The biological factors play major role in controlling the diversity (number of plants per m2) of this taxon. © 2015 College of Life Science. All rights reserved.


Srivastava D.,St. Joseph's College | Prabhuji S.K.,Biotechnology and Molecular Biology Center | Tripathi A.,KNIPSS | Srivastava R.,Biotechnology and Molecular Biology Center | Mishra P.,KNIPSS
Medicinal Plants | Year: 2012

The aqueous, ethanol and methanol fruit extracts of Flacourtia jungomas (Lour.) Raeus. have been tested for antibacterial activities against bacterial strains, viz., Salmonella typhimurium, Pseudomonas aeroginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis. All the three fruit extracts of Flacourtia jungomas possessed antibacterial activities against all the tested bacterial strains. Ethanol and methanol extracts were more potent than the aqueous extract which indicates that the more effective phytochemical is organic solvent soluble and needs to be explored further.


Shukla P.,Udai Pratap Autonomous college | Singh S.,KNIPSS | Dubey P.,KNIPSS | Singh A.,Udai Pratap Autonomous college | Singh A.K.,Udai Pratap Autonomous college
Ecotoxicology and Environmental Safety | Year: 2015

The role of nitric oxide (NO) as a key molecule in the signal transduction pathway of a biotic stress response has already been described. Recent studies indicate that it also participate in the signaling of abiotic stresses. In the present study, we showed the altered expression of stress responsive gene alternative oxidase (Aox1) in seedlings of barley (. Hordeum vulgare L.) in response to arsenic toxicity. Arsenic toxicity decreased the germination percentage, biomass, chlorophyll and carotenoid content whereas, arsenic toxicity enhanced the MDA content and proline content in a dose dependent manner. Other enzyme activities like catalase and superoxide dismutase increased with the increase in concentrations but it fell down at higher concentration of arsenic. Pretreatment of nitric oxide results in the enhanced expression of alternative oxidase which showed the adaptation of alternative pathway during the arsenic stress and it also enhances the growth ability and adaptability towards the arsenic stress. The results support the conclusion that nitric oxide ameliorates the arsenic toxicity not only at the level of antioxidant defense but also by affecting other mechanism of detoxification. © 2015 Elsevier Inc.


PubMed | KNIPSS and Udai Pratap Autonomous college
Type: | Journal: Ecotoxicology and environmental safety | Year: 2015

The role of nitric oxide (NO) as a key molecule in the signal transduction pathway of a biotic stress response has already been described. Recent studies indicate that it also participate in the signaling of abiotic stresses. In the present study, we showed the altered expression of stress responsive gene alternative oxidase (Aox1) in seedlings of barley (Hordeum vulgare L.) in response to arsenic toxicity. Arsenic toxicity decreased the germination percentage, biomass, chlorophyll and carotenoid content whereas, arsenic toxicity enhanced the MDA content and proline content in a dose dependent manner. Other enzyme activities like catalase and superoxide dismutase increased with the increase in concentrations but it fell down at higher concentration of arsenic. Pretreatment of nitric oxide results in the enhanced expression of alternative oxidase which showed the adaptation of alternative pathway during the arsenic stress and it also enhances the growth ability and adaptability towards the arsenic stress. The results support the conclusion that nitric oxide ameliorates the arsenic toxicity not only at the level of antioxidant defense but also by affecting other mechanism of detoxification.

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