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Broek in Waterland, Netherlands

Ermens A.A.,Klinisch Chemisch en Hematologisch Laboratorium
Nederlands tijdschrift voor geneeskunde | Year: 2011

A 1-year-old Moroccan boy was referred because of jaundice. A peripheral blood smear showed 'blister cells'. This finding is characteristic for haemolysis caused by glucose-6-phosphate dehydrogenase deficiency. It appeared hemolysis occurred because the boy ate fava beans. Source


Bos J.,Technical University of Delft | De Jonge N.,Klinisch Chemisch en Hematologisch Laboratorium | Veeke H.P.M.,Technical University of Delft
Nederlands Tijdschrift voor Klinische Chemie en Laboratoriumgeneeskunde | Year: 2014

The insourcing and outsourcing of diagnostic tests has a relatively large impact on the workload for clinical laboratories. The goal of this study is to gain insight in the logistical system of orders between clinical laboratories in the Netherlands. We observed the process and interviewed employees in three clinical laboratories, a transporter and a transportation service provider. We performed the analysis according to the Delft Systems Approach. The logistical system is subject to many requirements. However, evaluating the system on quantitative performance measures is not possible because of the lack of systematic feedback. The absence of unified protocols necessitates supplementing order information and gives rise to a relatively large workload for both the outsourcing and insourcing laboratory. Adding time and error registration will enable evaluation of the logistical system based on throughput times, throughput volume and success rate. Implementation of unified protocols can reduce the workload of the logistical system. © 1997-2014 Nederlandse V ereniging voor Klinische Chemie en Laboratoriumgeneeskunde. Source


van Rossum A.P.,Klinisch Chemisch en Hematologisch Laboratorium
Nederlands tijdschrift voor geneeskunde | Year: 2011

A 77-year-old man with dyspnoea was suspected to have a decompensatio cordis by the general practitioner. A diuretic was prescribed. Additional radiological and laboratory investigation (e.g. natriuretic peptides and D-dimers) showed pulmonary embolism instead of heart failure. A second patient, a woman aged 79 years, with a history of leukaemic mantle cell lymphoma, was treated with poly-chemotherapy (R-CHOP), after which remission was achieved. Four years later the lymphoma recurred and R-CHOP treatment was started. However this was without success, after which R-CHOP treatment was repeated. Subsequently the patient developed dyspnoea and pneumonia. Following additional radiological and laboratory investigation (e.g. natriuretic peptides) the patient was finally diagnosed with doxorubicin-induced heart failure. Based upon these case studies, the role of brain-natriuretic peptides in the differential diagnostic work-up of dyspnoea is highlighted. Test performance, correlation with disease, monitoring, prognostics, differential diagnostic power, reference values and pitfalls of brain natriuretic peptides are discussed. Source


Pandit A.,Leiden University | Wawrzyniak P.K.,Leiden University | Van Gammeren A.J.,Leiden University | Van Gammeren A.J.,Klinisch Chemisch en Hematologisch Laboratorium | And 3 more authors.
Biochemistry | Year: 2010

Protein nuclear magnetic resonance (NMR) secondary chemical shifts are widely used to predict the secondary structure, and in solid-state NMR, they are often the only unambiguous structural parameters available. However, the employed prediction methods are empirical in nature, relying on the assumption that secondary shifts are only affected by shielding effects of neighboring atoms. We analyzed the secondary shifts of a photosynthetic membrane protein with a high density of chromophores and very tight packing, the lightharvesting 2 (LH2) complex of Rhodopseudomonas acidophila. A relation was found between secondary shift anomalies and protein-protein or pigment-protein tertiary and quaternary contacts. For several residues, including the bacteriochlorophyll- coordinating histidines (αH31 and βH30) and the phenylalanine αF41 that has strongly twisted Cb-Ca-C and C a-C-N conformations in the LH2 crystal structure, the perturbing effects on the backbone chemical shifts were tested by density functional theory (DFT) calculations. We propose that higher-order interactions in the tightly packed complex can induce localized perturbations of the backbone conformation and electronic structure, related to functional pigment-protein or protein-protein interactions. ©2009 American Chemical Society. Source


Schumann G.,Institute For Klinische Chemie | Klauke R.,Institute For Klinische Chemie | Canalias F.,Autonomous University of Barcelona | Bossert-Reuther S.,Roche Holding AG | And 7 more authors.
Clinical Chemistry and Laboratory Medicine | Year: 2011

This paper is the ninth in a series dealing with reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 °C and the certification of reference preparations. Other parts deal with: Part 1. The concept of reference procedures for the measurement of catalytic activity concentrations of enzymes; Part 2. Reference procedure for the measurement of catalytic concentration of creatine kinase; Part 3. Reference procedure for the measurement of catalytic concentration of lactate dehydrogenase; Part 4. Reference procedure for the measurement of catalytic concentration of alanine aminotransferase; Part 5. Reference procedure for the measurement of catalytic concentration of aspartate aminotransferase; Part 6. Reference procedure for the measurement of catalytic concentration of γ-glutamyltransferase; Part 7. Certification of four reference materials for the determination of enzymatic activity of γ-glutamyltransferase, lactate dehydrogenase, alanine aminotransferase and creatine kinase at 37 °C; Part 8. Reference procedure for the measurement of catalytic concentration of α-amylase. The procedure described here is derived from the previously described 30 °C IFCC reference method. Differences are tabulated and commented on in Appendix 1. © 2011 by Walter de Gruyter Berlin Boston. Source

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