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Schmolke S.,Orthopadische Klinik der Medizinischen Hochschule Hanover im Diakoniekrankenhaus Annastift gGmbH | Zaremba D.,Leibniz University of Hanover | Biskup C.,Leibniz University of Hanover | Andreae A.,Klinik fur Kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik | Pude F.,ETH Zurich
Fuss und Sprunggelenk | Year: 2015

Hintergrund: Material und Methoden: Ergebnisse: Schlussfolgerung: Background: Interference screws are state of the art of bone-tendon-bone fixation in anterior-cruciate-ligament (ACL) reconstruction. These screws normally consist of materials like different polylactic acids because of their biodegradability. In this paper our first investigation of machining interference screws made of bovine bones by means of an abrasive waterjet is presented. The material bone as an implant has the advantage to be resorbed and replaced by new own bone tissue. Material and methods: In this paper it is shown that an abrasive waterjet is able to machine the whole interference screw's geometry with different process's steps including drilling, turning, manufacturing a screw thread profile and an inside contour as a drive system. The developing of the thread profile by overlapping the abrasive waterjet's trace will be focused in this paper as well as the manufacturing of a hexagonal inside contour as a drive system. In a comparative study, the osseous integration of xenogenic bone and commercially available interference screws of poly (L-lactide) interference screws were comparatively tested in-vivo. As a key feature the screws were tested at a scale of 1: 1. Results: The bovine bone screws have been fully integrated into the host osseous bone within the examination time of 270 days. Already after 30 days ingrowing trabeculae could be detected in the screw body. Conclusions: Based on this positive experiences with bovine bone screws the development of screws for use in the forefoot or midface area seems to be possible. © 2015. Source


Baums C.G.,Institute For Mikrobiologie | Bruggemann C.,Institute For Mikrobiologie | Kock C.,Institute For Mikrobiologie | Beineke A.,Institute For Pathologie | And 2 more authors.
Clinical and Vaccine Immunology | Year: 2010

Streptococcus suis is an important porcine pathogen causing meningitis and other invasive diseases in piglets of different ages. Application of S. suis serotype 2 bacterins to specific-pathogen-free (SPF) weaning piglets has been demonstrated to protect against the homologous serotype. However, autogenous S. suis bacterins are also applied to sows and suckling piglets in the field. Therefore, comparative evaluation of different bacterin immunization regimes, including sow vaccination, was performed in this study. The main objectives were to determine the immunogenicity of an S. suis bacterin in sows prepartum and its influence on active immunization of piglets. Experimental infection of 6- and 8-week-old weaning piglets was performed to elucidate protective efficacies. Humoral immune responses were investigated by an enzyme-linked immunosorbent assay (ELISA) measuring muramidase-released protein (MRP)-specific IgG titers and by opsonophagocytosis assays. Bacterin application elicited high MRP-specific IgG titers in the serum and colostrum of sows, as well as opsonizing antibodies. Piglets from vaccinated sows had significantly higher MRP-specific titers than respective piglets from nonvaccinated sows until 6 weeks postpartum. Vaccination of suckling piglets did not result in high MRP-specific titers nor in induction of opsonizing antibodies. Furthermore, neither vaccination of suckling nor of weaning piglets from immunized sows was associated with a prominent active immune response and protection at 8 weeks postpartum. However, protection was observed in respective 6-week-old weaning piglets, most likely because of protective maternal immunity. In conclusion, this study provides the first results suggesting protective passive maternal immunity for S. suis serotype 2 after bacterin vaccination of sows and a strong inhibitory effect on active immunization of suckling and weaning piglets, leading to highly susceptible growers. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Source


Humann-Ziehank E.,Klinik fur Kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik | Ganter M.,Klinik fur Kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik | Michalke B.,Helmholtz Center Munich
Journal of Trace Elements in Medicine and Biology | Year: 2016

This study was performed to characterise selenium (Se) and Se species in cerebrospinal fluid (CSF) of sheep and its relation to the respective Se concentrations in serum. Paired samples from 10 adult sheep were used for the study. Five sheep were fed a diet with a marginal Se concentration of <0.05mg Se/kg diet dry weight (dw, Se-), and five animals were fed the same diet supplemented with sodium selenite revealing a concentration of 0.2mg Se/kg diet dw (Se+). The feeding strategy was conducted for two years; The results on metabolic effects were published previously. At the end of the feeding period, paired samples of serum and CSF were collected and analysed using ion exchange chromatography inductively coupled plasma-dynamic reaction cell-mass spectrometry (IEC-ICP-DRC-MS) technique for total Se concentration and concentrations of Se species. Albumin concentrations were analysed additionally.The feeding strategy caused significant differences (p<0.01) in serum Se concentrations with 33.1±5.11μg Se/l in the Se- group and 96.5±18.3μg Se/l in the Se+ group, respectively. The corresponding total Se concentrations in CSF were 4.38±1.02μg Se/l and 6.13±1.64μg Se/l in the Se- and the Se+ group, respectively, missing statistical significance (p=0.077). IEC-ICP-DRC-MS technique was able to differentiate the Se species selenoprotein P-bound Se (SePP), selenomethionine, glutathione peroxidase-bound Se (Se-GPx), selenocystine, thioredoxin reductase-bound Se, ovine serum albumin-bound Se (Se-OSA), SeIV and SeVI in ovine serum and CSF. Quantitatively, SePP is the main selenoprotein in ovine serum followed by Se-GPx. The CSF/blood ratio of albumin (QAlbumin) reflected a physiological function of the blood-CSF barrier in all sheep. QSe-species were higher than QAlbumin both feeding groups, supporting the hypothesis of local production of Se species in the brain. Significant positive regression lines for CSF vs. serum were found for albumin and Se-OSA only, suggesting a role of albumin to convey Se across the blood-CSF barrier. The ovine model, together with the IEC-ICP-DRC-MS technique to characterise the Se species, might be a worthwhile model for further studies as repeated sample collection as well as modification of the nutritional status is feasible and effective. © 2015 Elsevier GmbH. Source


Von Altrock A.,Klinik fur Kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik | Beyerbach M.,Institute For Biometrie | Roesler U.,University of Leipzig | Hamedy A.,University of Leipzig | Waldmann K.-H.,Klinik fur Kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik
Archiv fur Lebensmittelhygiene | Year: 2010

Within the scope of a study on the occurrence of Campylobacter spp. in fattening herds and on pig liver surfaces, an assessment of parameters influencing the herd prevalence was performed on the basis of bacteriological and serological findings. Especially herd factors were considered in connection with herd prevalence. By means of an in-house ELISA, reactors were detected in all of the 80 serologically investigated herds. The intra-herd prevalence was between 63.3 % and 100 %. Campylobacter spp. were found on 9.8 % (n=147) of 1,500 bacteriologically investigated pig liver surfaces. Altogether, livers from 28 % (n=14) of 50 herds included in this investigation were Campylobacter negative. Statistically, bacteriological prevalence was higher in herds when clothes and boots were not changed before entering the stable. A higher serological prevalence was found in herds where diseased pigs were housed with healthy pigs in the same compartment and in herds with high loss rate in comparison to all investigated pig herds. © M. & H. Schaper GmbH & Co. Source


Strauch-Surken L.,Klinik fur Kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik | Wendt M.,Klinik fur Kleine Klauentiere und Forensische Medizin und Ambulatorische Klinik
Tierarztliche Praxis Ausgabe G: Grosstiere - Nutztiere | Year: 2015

Objective: On a conventionally managed piglet-producing farm, novel infrared (IR) heating plates for piglets in the farrowing pens were tested for their suitability and compared with common warm-water (WW) heating plates. Material and methods: In total, 134 litters (summer n = 82, winter n = 52) were investigated, which were housed on IR or WW heating plates, respectively, with or without an extra cover plate (groups 1-4). To determine the influence of the different heat sources, the wound healing after castration and tail docking, the umbilical regression and the weight gain of the piglets were investigated. Additionally, the lying behavior of the piglets and the position of the sows' udder at the time of farrowing were examined with regard to the heating plates. Furthermore, the energy consumption and costs were compared. Results: The piglets housed on IR heating plates displayed better wound healing after castration and tail docking than the piglets housed on WW plates. The best results were obtained in piglets kept on IR heating plates with an extra cover plate. In addition, significant benefits were demonstrated for the usage of IR heating plates regarding umbilical regression. The piglets kept on IR heating plates had a slightly better weight gain in summer, whereas there were no differences between groups during winter. The lying behavior in the creep areas was similar in all groups. In general, with increasing age the percentage of time piglets spent in the lying position on the plates decreased. The percentage of time lying on the plates was higher in winter than in summer. At farrowing, 74.6% of all investigated sows directed their udder towards the heating plates. With the IR heating plates, this behavior occurred significantly more often. The energy consumption (kWh) per litter was significantly lower for the IR heating plates (electric power) both in winter and summer in comparison with the WW plates (gas). The energy costs were comparable in summer, but were higher for the IR heating plates in winter. Conclusion and clinical relevance: IR radiation of heating plates has a positive influence on wound healing after castration and tail docking as well as on umbilical regression. In addition, the lying behavior of sows at farrowing is influenced by the IR radiation. Despite the lower energy consumption of the IR heating plates, the most profitable system has to be determined individually for each farm. © Schattauer 2015. Source

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