Assessment of serum sialic acid and oxidative stress parameters in rheumatoid arthritis patients receiving anti-TNF-α and conventional therapies [Anti-TNF-α ve Geleneksel Tedavi Alan Romatoid Artritli Hastalarda Serum Sialik Asit ve Oksidatif Stres Parametrelerinin Deǧerlendirilmesi]
Erer B.,Umraniye Egitim ve Arastirma Hastanesi |
Yazici S.,Duzce University |
Yilmaz G.,Klinik Biyokimya Laboratuvar |
Yilmaz F.M.,Klinik Biyokimya Laboratuvar |
And 2 more authors.
Turkiye Fiziksel Tip ve Rehabilitasyon Dergisi | Year: 2010
Objective: In this study, we aimed to evaluate sialic acid (SA) levels and oxidative stress parameters by measuring nitric oxide metabolites (NOx), thiobarbituric acid-reactive substances (TBARS) and thiol (S) in rheumatoid arthritis (RA) patients treated with either conventional or anti-tumor necrosis factor-α (anti-TNF-α) therapy. Materials and Methods: Eighty-six RA patients and 20 healthy controls were enrolled in the study. All RA patients fulfilled the American College of Rheumatology (ACR) criteria for RA. Thirty patients were on anti-TNF-α therapy. The rest of the patients were either treated with two or three disease-modifying anti-rheumatic drugs. Health Assessment Questionnaire (HAQ) score and the Disease Activity Score 28 (DAS28) were calculated in all patients. NOx, TBARS, SA, and SH levels were measured in both patients and controls. Results: The SA levels were statistically higher in RA patients than in controls. We found increased levels of TBARS and NOx and decreased levels of SH in both groups, but these results were statistically insignificant. Spearman correlation analysis revealed positive correlation between serum TBARS levels and both NOx (r=0.322, p=0.001) and SA (r=0.242, p=0.017) levels. Conclusion: Our results confirm the role of SA in the pathogenesis of RA and its correlation with oxidative stress. Further studies on larger numbers of subjects and with longer treatment durations are needed to analyze the long-term effects of RA therapies on serum oxidative stress parameters. © Turkish Journal of Physical Medicine and Rehabilitation, Published by Galenos Publishing.
Bal C.,Klinik Biyokimya Laboratuvar |
Serdar M.A.,Gulhane Askeri Tp Akademisi |
Gungor O.T.,Klinik Biyokimya Laboratuvar |
Celik H.T.,Klinik Biyokimya Laboratuvar |
And 4 more authors.
Turkish Journal of Biochemistry | Year: 2014
Objective: The aim of this study is the calculation of measurement uncertainty values of ten different iochemical parameters by using internal and external quality control datas with three different, but same model and trademark device and the comparison of these values with Fraser’s and CLIA’s total allowable error % (TEa%) values. Methods: In the calculation of measurement uncertainty, six step “uncertainty calculation model”, that is defined in Nordest guide which is based on European Accreditation Guideline/12 /, European Technical Report: 1/3/and ISO/DTS 21748 Guideline/8/was used. Results: TEa% values of blood urea nitrogen for Device A, potassium values for Device B and albumin, creatinine, sodium and total protein values for Device C were found to be higher when compared to TEa% values of Fraser. TEa% of blood urea nitrogen, which has been calculated for Device A, B and C was found to be higher when compared to TEa% values of CLIA. TEa% values which has been calculated for glucose, AST, cholesterol and triglyceride in each three device was not found to be higher than TEa% values of CLIA and Fraser. Conclusion: Laboratories should establish the model for calculation of uncertainity measurement and evaluation criterias and take the analytical difference between devices under control. Also they should give the results which are not exceeding the targeted TEa% values and should inform the clinicians about it. © TurkJBiochem.com.