Kitii Co.

Shinjuku, Japan

Kitii Co.

Shinjuku, Japan
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Tobita K.,Shinshu University | Tobita K.,Kitii Co. | Yanaka H.,Kitii Co. | Otani H.,Shinshu University
Journal of Nutritional Science and Vitaminology | Year: 2010

We investigated the effect of Lactobacillus crispatus KT-11 (KT-11) on intestinal immune systems in C3H/HeN mice. The level of intestinal total immunoglobulin (Ig) A was significantly higher in mice given KT-11 than in mice not given KT-11. Gene expression relating to antibody production and innate immune response increased more than 2-fold in the former compared with the later. Moreover, the number of IL-6 + CD11b+cells was significantly higher in Peyer's patch cells cultured with KT-11 than in those cultured without KT- 11, although the number of CD4 +cells and the cell ratio of CD4+/CD8+ were remarkably lower in the culture with KT-11. These results indicate that KT-11 enhances intestinal IgA production and innate immune response in C3H/HeN mice.


Tobita K.,Shinshu University | Tobita K.,Kitii Co. | Yanaka H.,Kitii Co. | Otani H.,Shinshu University
Animal Science Journal | Year: 2010

In this study, we investigated the effects of oral ingestion of Lactobacillus crispatus KT-11 strain (KT-11) on the immune response in an allergic rhinitis mouse model, ovalbumin (OVA)-sensitized BALB/c mice. Sneezing activity in mice that were administered a KT-11-supplemented diet was significantly lower than that in mice administered a KT-11-free diet (control diet) at age 11 weeks. We found that serum OVA-specific immunoglobulin E (IgE) levels and total number of interleukin (IL)-4+CD4+ spleen cells in mice that were administered a KT-11-supplemented diet were significantly lower than in mice administered a control diet. The ratio of spleen interferon-γ+CD4+/IL-4+CD4+ cells was higher in the mice administered the KT-11-supplemented diet compared to that in mice administered the control or L. rhamnosus GG-supplemented diet. In contrast, the number of CD11b+CD80+ and FcεRIα+CD117+ cells was significantly lower in mice administered the KT-11-supplemented diet. These results suggested that KT-11 reduced OVA-induced allergic symptoms in BALB/c mice via the adjustment of the T helper type 1/T helper type 2 balance, and a decrease in the number of antigen-presenting cells and high affinity IgE receptor-positive mast cells. © 2010 The Authors. Journal compilation © 2010 Japanese Society of Animal Science.


Tobita K.,Shinshu University | Tobita K.,Kitii Co. | Yanaka H.,Kitii Co. | Otani H.,Shinshu University
Journal of Agricultural and Food Chemistry | Year: 2010

We synthesized a medium consisting of commercial food supplements (food grade medium) that could be used to cultivate Lactobacillus crispatus KT-11 (KT-11), and investigated the antiallergic effects and acute toxicity of KT-11 cultured in this medium. We found that the growth of KT-11 in the food grade medium was comparable to that in DeMan-Rogosa-Sharpe (MRS) medium. Sneezing event was reduced in ovalbumin (OVA)-sensitized BALB/c mice given a diet supplemented with KT-11 grown in the food grade medium (FG-KT-11 group) when compared to mice given a diet supplemented with KT-11 grown in MRS medium (MRS-KT-11 group). The number of CD80+CD11b+ Peyers patch cells was significantly lower in the FG-KT-11 group than in the MRS-KT-11 group, while IL-12+CD11b+ Peyers patch cells were higher in the FG-KT-11 group. Only minimal acute toxicity was observed in ICR mice given 1000 or 2000 mg of FG-KT-11/kg body weight. These results suggest that FG-KT-11 represents a safe antiallergic food material. © 2010 American Chemical Society.


The effects of heat-treated Lactobacillus (Lb.) crispatus KT-11 on influenza virus infection and macrophage cytokine production in mice were investigated. The mice were orally administered saline containing heat-treated Lb. crispatus KT-11 (0, 1 or 10 mg). Then, the mice were intranasally inoculated with influenza H1N1 virus and assessed for survival rate and changes in body weight. The mouse macrophage cell line RAW264 was cultured in the presence of heat-treated lactic acid bacteria for 48 hours. Cytokine levels in the cell culture supernatant were determined using ELISA. The area under the curve of changes in body weight (AUCW) in mice infected with influenza virus and orally administrated heat-treated Lb. crispatus KT-11 (1 mg) was significantly higher than that in mice not administered any bacterium (control group). The concentrations of IFNα, TNFα, IL-1α and IL-6 were higher in the RAW264 cells cultured with heat-treated Lb. crispatus KT-11 than in control (non-addition of bacteria) or in those with other heat-treated bacteria. These results suggest that heat-treated Lb. crispatus KT-11 enhances macrophage cytokine production and protects against influenza virus infection. Copyright © 2015, Japanese Society for Food Science and Technology.


Patent
Kitii Co. | Date: 2011-03-04

It is to provide a composition derived from lactic acid bacteria having an anti-allergic effect (an effect of suppressing allergic symptoms such as hay fever) synergistically, and a food product, pet food, etc. containing the composition. It is to prepare a composition having an anti-allergic action, containing Lactobacillus crispatus KT-11 strain, KT-23 strain, or KT-25 strain bacterial cells and viable bacteria, dead bacteria, or a processed product of bacterial cells of other lactic acid bacteria belonging to the genus Lactobacillus, genus Bifidobacterium, genus Leuconostoc, genus Enterococcus, genus Pediococcus, etc. at a biomass-converted ratio of 99:1 to 50:50, preferably 99:1 to 75:25.


Patent
Kitii Co. | Date: 2013-01-23

It is to provide a composition derived from lactic acid bacteria having an anti-allergic effect (an effect of suppressing allergic symptoms such as hay fever) synergistically, and a food product, pet food, etc. containing the composition. It is to prepare a composition having an anti-allergic action, containing Lactobacillus crispatus KT-11 strain, KT-23 strain, or KT-25 strain bacterial cells and viable bacteria, dead bacteria, or a processed product of bacterial cells of other lactic acid bacteria belonging to the genus Lactobacillus, genus Bifidobacterium, genus Leuconostoc, genus Enterococcus, genus Pediococcus, etc. at a biomass-converted ratio of 99:1 to 50:50, preferably 99:1 to 75:25.

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