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Yokohama-shi, Japan

Ishibashi T.,Kirin Brewery Co.
Nihon Arukōru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence | Year: 2010

It is generally assumed that subjects with ALDH2*1/1 do not exhibit flushing symptoms after alcohol intake, but recent studies have revealed discordance in ALDH2 genotypes with self-reported facial flushing and the ethanol patch test. We evaluated the reliability of a facial flushing questionnaire and the ethanol patch test in 495 Japanese volunteers. Genomic DNA was extracted from peripheral blood samples and amplified by polymerase chain reaction (PCR) to detect ALDH2 polymorphisms as reported previously. The subjects were classified by the presence or absence of facial flushing using a questionnaire. Ethanol patch tests were performed using the previously described method. Each subject's alcohol consumption was established through a questionnaire. In each gender, there was a high frequency of facial flushing and positive ethanol patch tests in ALDH2*1/1 subjects. Alcohol consumption was not significantly different between ALDH2*1/1 subjects with positive and negative results. However, alcohol consumption in the positive ALDH2*1/1 group was significantly higher than that of ALDH2*1/2 subjects in both tests, indicating that the positive results may lead to variance with ALDH2*1/1. Most of the ALDH2*1/2 subjects exhibited facial flushing and a positive patch test. Meanwhile, 100% in the ALDH2*2/2 group exhibited positive results. There was no significant gender difference in either facial flushing or ethanol patch test reactions. In addition, the frequency of both male and female ALDH2*1/1 subjects with positive flushing and ethanol patch test reactions increased roughly in proportion to drinking frequency, but no significant differences were observed between them. Consequently, the general use of self-reported facial flushing or ethanol patch tests instead of ALDH2 genotyping should be carefully handled for genetic association studies of drinking behavior and alcohol-related diseases.

Kage T.,KAGE Consulting Office | Kage T.,Kirin Brewery Co.
Journal of the Vacuum Society of Japan | Year: 2015

The unique properties of diamond like carbon (DLC) film, including its chemical inertness and impermeability, make it possible for new applications in food and beverage. In this paper, I summarize and review the recent progress of gas barrier enhancement of polyethylene terephthalate (PET) bottles for beverage from the view point of the application of DLC coating. A unique technique of plasma CVD method has been developed to deposit DLC layer on the inside surface of PET bottles. The DLCcoated PET bottle exhibits extremely high gas barrier properties against oxygen, carbon dioxide and flavors compared to conventional bottles. For the practical use of PET bottles as a commodity, high speed and low cost coating are essential; accordingly several high speed coating machines with a capacity of up to 18000 bottles per hour have been developed, and successfully operated so far for a large production of soft drink. Low speed machines have also been introduced for wine, Japanese sake, edible oil, seasonings and others.

Watanabe S.,Kirin Brewery Co. | Seki H.,Osaka Institute of Technology | Ishii H.,Kwansei Gakuin University
IEEE International Conference on Industrial Informatics (INDIN) | Year: 2010

The single input rule modules connected fuzzy inference method (SIRMs method) can decrease the number of fuzzy rules drastically in comparison with the conventional fuzzy inference methods. Moreover, Seki et al. have proposed a functional type single input rule modules connected fuzzy inference method which generalizes the consequent part of the SIRMs method to function. However, these SIRMs methods can not realize XOR (Exclusive OR). Therefore, Seki et al. have proposed a "neural network-type SIRMs method" which unites the neural network and SIRMs method, and shown that this method can realize XOR. In this paper, neural network-type SIRMs method is shown to be superior to the conventional SIRMs method and neural network by applying to a medical data and Iris data. © 2010 IEEE.

Omasa T.,Osaka University | Furuichi K.,Osaka University | Furuichi K.,Meiji Dairies Corporation | Iemura T.,Osaka University | And 4 more authors.
Bioprocess and Biosystems Engineering | Year: 2010

Generally, mammalian cells utilize glucose and glutamine as primary energy sources. To investigate the effect of energy sources on metabolic fluxes and antibody production, glucose- or glutamine-limited serum-free continuous culture of hybridoma 3A21 cells, which produce anti-ribonuclease A antibody, was carried out. The cell volume and dry cell weight were evaluated under various steady-state conditions. The specific consumption and production rates were evaluated on the basis of dry cell weight. On the basis of these results, the fluxes of the metabolic pathway were calculated. It was found that increasing the specific growth rate causes the specific ATP and antibody production rates to decrease. The fluxes between malate and pyruvate also decreased with the increase in specific growth rate. To increase the ATP production rate under steady-state conditions by the enhancement of fluxes between malate and pyruvate, the reduced metabolic fluxes were increased by an intermediate (pyruvate, malate, and citrate) addition. As a result, higher specific ATP and antibody production rates were achieved following the intermediate addition at a constant dilution rate. © 2009 Springer-Verlag.

Kinouchi T.,Kirin Company Ltd | Komeda T.,Kirin Brewery Co. | Nakanishi K.,Kirin Company Ltd | Fujita Y.,Kirin Company Ltd | Deuchi K.,Kirin Company Ltd
Biocontrol Science | Year: 2014

Alicyclobacillus acidoterrestris, an obligate aerobe and one of the most harmful bacteria in acidic beverages, requires oxygen for growth. However, the relationship between oxygen availability and its growth has not yet been quantified. We examined the correlation between A. acidoterrestris growth and oxygen availability to determine whether A. acidoterrestris can be controlled by restricting oxygen. Airtight containers were filled with YSG broth and apple juice at various oxygen concentrations. Positive correlation R 2=0.9329 was observed between A. acidoterrestris growth and oxygen availability in YSG broth, and a lower but nonetheless slight correlation R 2=0.5604 was observed for apple juice. These results indicate that decreased oxygen availability in a container could restrict growth. As results, the addition of reducing compounds along with airtight conditions may help prevent the deterioration of beverages caused by the proliferation of A. acidoterrestris.

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