Bayar Muluk N.,Cankaya University |
Arikan O.K.,Kinkkale University |
Atasoy P.,Adana Numune Research and Training Hospital |
Kilic R.,Cankaya University |
Tuna Yalcinozan E.,Artvin State Hospital
B-ENT | Year: 2013
Inducible nitric oxide synthase (iNOS) in sinonasal polyp pathogenesis. Objectives: We investigated the role of inducible nitric oxide synthase (iNOS) in the pathogenesis of sinonasal polyps. Methods: Adult patients (21 men, 3 women) with nasal polyposis underwent functional endoscopic sinus surgery. Nine adults without polyps (6 men) who underwent septoplasty and/or rhinoplasty served as controls. Polyp specimens came from three regions: the maxillary sinus (10), ethmoid sinus (14), and nasal cavity (10). Control group samples (9) came from the inferior turbinate. Specimens were evaluated in eight mucosal layers for count and distribution of inflammatory cells and iNOS expression. An iNOS positivity index (PI) was determined for the epithelium (E), subepithelial layer of the lamina propria (SE), and deep paraglandular layer of the mucosa (D). Results: Polymorphonuclear cell (PMNC) % values of the ethmoid and maxillary sinus and overall ethmoid sinus PI were significantly higher in the polyp group. Patients with longer polyp duration, D-perivascular (D-pv), and a higher Brinkmann index had decreased ethmoid sinus D Pis. However, in older patients and patients with longer polyp duration, perivascular Pis increased in maxillary sinus SE and D, respectively. Furthermore, as PMNC % and iNOS-PMNC PI increased, SE-glandular and epithelial-apical iNOS values decreased. In the ethmoid and maxillary sinuses, iNOS-D-endothelial values increased but decreased in the nasal cavity. Conclusions: iNOS may play a role in sinonasal polyp pathogenesis, especially in mucosal SE and D layers. Increased vascular permeability, stromal edema, inflammatory cell migration into the stroma of the mucosa, and increased mucosal gland secretion may result in polyp formation.
Ozuguz P.,Afyon Kocatepe University |
Kacar S.D.,Afyon Kocatepe University |
Akci O.,Afyon Devlet Hastanesi |
Balta I.,Kecioren Egitim Arastirma Hastanesi |
And 2 more authors.
Gulhane Medical Journal | Year: 2014
There is not any single laboratory finding as an indicator of activity of Behcet's Disease (BD) and various parameters are investigated in many different studies. The aim of our study is to evaluate the inflammation markers, neutrophil/lymphocyte ratio (NLR), thrombocyte/lymphoyte ratio (TLR) and coagulation markers MPV, MPV/thrombocyte ratio; and determine a timely, easy, inexpensive and fast activity marker for the disease. Eighty five patients with diagnosis of BD according to the Criteria International Behcet Disease Study Group and 40 age-sex matched controls are recruited to the study. Patients are divided into two groups as active patients and those under remission depending on the activity criteria. The complete blood count, ESR and CRP values are retrospectively scanned. Of the 85 patients 40 were active and 45 were under remission. The duration of disease were less than 1 year in 16.5%, 1-5 years in 50.6%, longer than 5 year in 32.9%. There was a statistically significant difference in MPV and NLR values between patient and control groups whereas Difference was not significant in MPV/thrombocyte and TLR values. When the active patients were compared with patients in remission ESR, CRP and NLR values were significantly higher in active patients. There were 6 patients with a history of deep venous thrombosis. Althought there were significantly higher values a statistical evaluation was not done due to the low patient number. BD constitutes a mutisystemic vasculitic process that as an inflammation marker, NLR is found to be high. Besides ESR and CRP, NLR can be used as an activity marker. MPV that indicates a vulnerability to thrombosis is higher in patients. Antithrombotic therapy may be required in patients with high MPV values. © Gülhane Askeri Tip Akademisi 2014.
Yildirim E.,Kinkkale University |
Aktuna Z.,Kinkkale University |
Yalcinkaya I.,Kinkkale University |
Eraslan G.,Erciyes University |
And 3 more authors.
Fresenius Environmental Bulletin | Year: 2013
This study was conducted to investigate the contractility of aflatoxicated isolated broiler ileum smooth muscles. Fourty-eight, one-day old broiler chicks were divided into 4 groups, each containing 12 chicks. The 1st group was the control group, the 2nd group received 1 g/kg yeast glucomannan (Mycosorb), the 3rd group received 2 mg/kg aflatoxin, and the 4th group received lg/kg yeast glucomannan + 2 mg/kg aflatoxin in the feed. At the 21st day of the study, chicks were decapitated and the ileum isolated. Decapitation lasted on 28th day of the study. The isolated ileum strips were mounted under a basal tension of 1 g. The contractions of acetylcholine (ACh), nicotine, and bethanechol were calculated as g contraction/mg wet weight of ileum. For histopathologic examination, ileum sections were stained with hematoxylin-eosin and masson trichrome. The isolated ileum muscle contractions with ACh, nicotine, and bethanechol were not statistically significant in the study groups as compared to control group (P>0.05). Pathological examination revealed no histopathological changes in the smooth muscle tissues of the study group chicks when compared with control group. It is concluded that aflatoxins do not specifically change the contractility of broiler chick ileum to agonists most likely due to lack of aflatoxin-in-duced pathologic changes in the broiler chick ileum. © by PSP.
Yildirim E.,Kirikkale University |
Macun H.C.,Kirikkale University |
Cinar M.,Kirikkale University |
Yigit A.A.,Kirikkale University |
And 3 more authors.
Clinical and Experimental Obstetrics and Gynecology | Year: 2014
Purpose: This study was undertaken to evaluate the effects of magnesium sulfate (MgSO4) on the contractile activity of the uterus in a pregnant rat model of preeclampsia induced by N-nitro-arginine methyl ester (L-NAME). Materials and Methods: Twenty-eight, 160-220 gram, three to four month old female Sprague-Dawley rats were used in this study. After conception was confirmed by vaginal smears on the first day of pregnancy, the animals were allocated into four groups according to the chemicals fed in their drinking water as control (nothing administered), L-NAME (50 mg/kg L-NAME), MgSO 4 (600 mg/kg MgSO4), and MgSO4 + L-NAME group (600 mg/kg MgSO4 + 50 mg/kg L-NAME). The pregnant uterus strips were isolated on the 19th day and the contractile activity of uterus was examined by applying 0, 0.1, 0.2, 0.4, 0.8, and 2.5 mIU/ml oxytocin to each group and responses are recorded accordingly. Results: There were no statistically significant differences regarding fetal parameters and peak amplitudes of the oxytocin stimulated pregnant rat myometrial strips among groups. In L-NAME group at 0 and 0.1 mIU/ml oxytocin, the contraction frequency in a ten-min period was statistically lower than the control group (Z= -2.850, p = 0.004; Z = -2.902, p = 0.004, respectively). In MgSO4 group only at 0 mlU/ml oxytocin, the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -2.973, p = 0.003). In L-NAME + MgSO4 group at 0, 0.1 and 0.2 mIU/ml oxytocin concentrations the frequencies of the contractions in ten-min period were statistically lower than the control group (Z= - 4.018, p = 0.000; Z= -3.237, p = 0.001; Z= -2.902, p = 0.004, respectively). In L-NAME + MgSO4 given group at each oxytocin concentrations, the frequencies of the contractions in ten-min period were lower but not statistically different than the L-NAME group. Conclusion: MgSO 4 has no significant effect on the amplitude of spontaneous or oxytocin induced myometrial contractions, but decreased the frequency of spontaneous contractions. At each doses of oxytocin, MgSO4 has no significant effect on the frequency of contraction in a pregnant rat model of preeclampsia induced by L-NAME.
Serpil O.,Kinkkale University |
Murat K.,Kinkkale University |
Nisa T.,Kinkkale University |
Latif O.,Kinkkale University |
Ziya A.,The Hanover Cytopathology Institute
Academic Journal of Cancer Research | Year: 2010
Adenocarcinomas are the most common cause of malignancy in pleura fluids. Usual primary sites include the lung, breast, gastrointestinal tract and genitourinary tracts. Predicting the site of origin of an adenocarcinoma is difficult due to overlapping morphologic characteristics. Thyroid transcription factor-1 (TTF-1), Cytokeratin7 (CK7) and Cytokeratin8 (CK8) were investigated to differentiate the primary and metastatic lung adenocarcinoma in 14 pleura fluid samples. There was a significant difference between TTF-1, CK 7 and CK 8 expressions in primary lung adenocarcinomas (P=0.028; Chi-squared test) and in metastatic lung adenocarcinomas (P=0.004; Chi-squared test). The sensitivity of TTF-1, CK7 and CK8 as a marker for primary lung adenocarcinomas were 0, 87 and 57%, respectively. Moreover, the sensitivity of TTF-1, CK7 and CK8 as a marker for metastatic lung adenocarcinomas were 16, 86 and 75%, respectively. Overall results for primary lung adenocarcinomas demonstrated CK8 reactivity in 4 (57%) of 7 cases. Nine metastatic lung adenocarcinoma samples (75%) were positive for CK8. TTF-1 was expressed in 16% of metastatic lung adenocarcinomas, but negative TTF-1 staining was observed in all primary lung adenocarcinoma cases. It was concluded that these results confirmed that the method of TTF-1, CK7 and CK8 immunohistochemistry is not sensitive enough for the differential diagnosis of primary and metastatic lung adenocarcinomas. © IDOSI Publications, 2010.
Cilek S.,Kinkkale University |
Gotoh T.,Kyushu University
Journal of the Faculty of Agriculture, Kyushu University | Year: 2014
The effects of dam age, lamb gender, and singleton or twin status on lamb body size and growth were investigated in Malya lambs in Middle Anatolia, Turkey. Data were obtained from 302 lambs from an elite Malya sheep flock. Wither height, body length, rump height, rump length, back height, rump width, and front cannon bone circumference were recorded. The least square means method was used to determine factors that significantly affected body size of the lambs. General linear model in Minitab packet program was used to test the effects of variables on body size measurements. Least square means for wither height at birth and 1, 2, 3, 4, 6, 9 and 12 months of age were 40.13, 48.09, 55.07, 60.16, 64.99, 70.23, 74.45 and 76.66 cm, respectively. Dam age did not significantly affect body size parameters during the first year of life, except for rump height and front cannon bone circumference. Male lambs were significantly larger between birth and 12 months of age than females (P<0.05). Singleton lambs were significantly larger than twins until 6 months of age (P<0.05), but this effect was not significant after 6 months of age. It is suggested that there might be compensatory growth in twin lambs after weaning. In particular, twins born from 2-year-old dams show strong compensatory growth post-weaning. If farmers are able to ensure twin lamb survival until weaning age, they can expect compensatory growth after 9 months of age.