Nagoya-shi, Japan
Nagoya-shi, Japan

Kinjo Gakuin University is a private women's college in Moriyama-ku, Nagoya, Aichi Prefecture, Japan. The predecessor of the school was founded in 1889, and it was chartered as a university 1949. Wikipedia.

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Inoue K.,Kinjo Gakuin University | Hattori Y.,Kinjo Gakuin University | Hino T.,Kinjo Gakuin University | Oka H.,Kinjo Gakuin University
Journal of Pharmaceutical and Biomedical Analysis | Year: 2010

In the field of pharmaceutical and biomedical analysis of peptides, a rapid on-line detection and identification for a methodology have been required for the discovery of new biological active products. In this study, a high-speed counter-current chromatography with electrospray mass spectrometry (HSCCC/ESI-MS) was developed for the on-line detection and purification of polypeptide antibiotics of enramycin-A and -B. The analytes were purified on HSCCC model CCC-1000 (multi-layer coil planet centrifuge) with a volatile solvent of two-phase system composed of n-butanol/hexane/0.05% aqueous trifluoroacetic acid solution (43/7/50, V/V/V), and detected on an LCMS-2010EV quadrupole mass spectrometer fitted with an ESI source system in positive ionization following scan mode (m/z 100-2000). The HSCCC/ESI-MS peaks indicated that enramycin-A (major m/z 786 [M+3H]3+ and minor m/z 1179 [M+2H]2+) and enramycin-B (major m/z 791 [M+3H]3+ and minor m/z 1185 [M+2H]2+) have the peak resolution value of 2.9 from 15 mg of loaded enramycin powder. The HSCCC collected amounts of the peak fractions were additionally 4.3 mg (enramycin-A), and 5.9 mg (enramycin-B), respectively. These purified substances were analyzed by LC/ESI-MS with scan positive mode. Based on the LC/ESI-MS chromatograms and spectra of the fractions, enramycin-A and -B were estimated to be over 95% purity. The overall results indicate that this approach of HSCCC/ESI-MS is a powerful technique for the purification and identification of bioactive peptides. © 2009 Elsevier B.V. All rights reserved.

Ushida H.,Kinjo Gakuin University
5th ACM/IEEE International Conference on Human-Robot Interaction, HRI 2010 | Year: 2010

This paper describes about the effect of social robot's behavior on human performance. The robot behaves based on an artificial mind model, and it expresses emotions according to the situation. In this research, we consider about the case where human and the robot learn cooperatively. The robot emotionally reacts to the joint learner's success and failure. The experimental result shows that social behavior of the robot influences the performance of human learners. © 2010 IEEE.

Inoue K.,Kinjo Gakuin University | Dowell D.,AOAC INTERNATIONAL
Journal of AOAC International | Year: 2012

Official MethodSM 2011.21 is for the quantitation of the following nucleotides: adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), uridine 5'-monophosphate (UMP), cytidine 5'-monophosphate (CMP), and inosine 5'-monophosphate (IMP) in infant formula and adult/pediatric nutritional formula. It uses hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC-MS/MS). Preparation of the internal standards was conducted using centrifugal ultrafiltration and the standards are AMP-13C 10, 15N5; GMP-13C10, 15N5; UMP-13C9, 15N 2; and CMP-13C9, 15N3. Data were collected by using multiple reaction monitoring of the product ions of protonated molecules of the five nucleotides generated by positive-electrospray ionization. The HILIC conditions were conducted with ammonium formate (30 mmol/L) in water (pH 2.5, adjusted with formic acid) and methanol. The LOD and LOQ of the standard solution were 0.005-0.01 and 0.01-0.03 μg/mL, respectively. Recovery data were collected for intraday and interday testing and ranged from 98.1 to 108.9% with an RSD of 0.7-5.4%. The analytical range of the method is between 0.04 to 5 μg/mL for standard solution. © 2012 Publishing Technology.

Kataoka T.,Yokohama College of Pharmacy | Watanabebe S.,Kinjo Gakuin University
Heterocycles | Year: 2011

Alkynyl- and alkenylselenonium salts reacted with nucleophiles at the α- or β-carbon depending upon the nucleophiles. The α-attack caused the addition-elimination reaction i.e., the apparent substitution reaction and the β-attack (the conjugate addition) generated an ylide, which brought about the tandem reaction to form a variety of heterocyclic compounds. Some new reactions proceeded via the selenuranes formed by the attack of a nucleophile on the positively charged selenium atom. An interesting tandem Michael-aldol reaction of enones (ynones) bearing a chalcogenide or a thioamide was developed. The reactions of the 1-[2-(methylchalcogeno)phenyl]propenones gave α-(α-hydroxyalkyl)enones (Morita-Baylis-Hillman adducts) after a work-up with Et 3N. The reactions of the 3-cinnamoyl-1,3- oxazolidine-2-thiones with aldehydes gave tricyclic compounds with a bridgehead bound to four heteroatoms. The asymmetric reactions simultaneously induced four stereocenters, three of which are contiguous. Removal of the chiral auxiliary provided 1,3-diols bearing three consecutive stereocenters. © The Japan Institute of Heterocyclic Chemistry.

Inoue K.,Kinjo Gakuin University | Obara R.,Kinjo Gakuin University | Hino T.,Kinjo Gakuin University | Oka H.,Kinjo Gakuin University
Journal of Agricultural and Food Chemistry | Year: 2010

A method for the quantitation of nucleotides (adenosine 5′-monophosphate (AMP), guanosine 5′-monophosphate (GMP), uridine 5′-monophosphate (UMP), cytidine 5′-monophosphate (CMP), and inosine 5′-monophosphate (IMP)) in infant formula was developed by hydrophilic interaction chromatography with tandem mass spectrometry (HILIC-MS/MS). The internal standards used (AMP-13C10, 15N 5; GMP-13C10, 15N5; UMP-13C9, 15N2; CMP- 13C9, 15N3) were prepared with centrifugal ultrafiltration (CUF). Data acquisition was achieved by using multiple reaction monitoring (MRM) of product ions of protonated molecules of the five nucleotides generated by the positive-ion ESI. HILIC conditions were performed with 30 mmol/L ammonium formate in water (pH 2.5, adjusted with formic acid) and methanol. The LOD and LOQ were 5-10 μg/mL and 10-30 μg/mL for standard solution, respectively. Recovery for intra-and interday assays ranged from 98.1 to 108.9% (RSD: 0.7-5.4%) spiked with three concentration levels (5, 25, and 250 μg/g powder infant formula). This method could be applied for the determination of nucleotides in infant formula samples. The detected concentrations of five nucleotides ranged from not detected (n.d.) to 278 μg/g powder infant formula. The total nucleotide level ranged from n.d. to 600.2 μg/g powder infant formula. © 2010 American Chemical Society.

Ikemura K.,Kinjo Gakuin University | Yamamoto M.,Mie University | Miyazaki S.,Kinjo Gakuin University | Mizutani H.,Kinjo Gakuin University | And 2 more authors.
Molecular Pharmacology | Year: 2013

P-glycoprotein (P-gp/MDR1) is a multispecific efflux transporter regulating the pharmacokinetics of various drugs. Although P-gp expression in the small intestine is elevated after liver ischemia-reperfusion (I/R) injury, the regulatory mechanism remains to be clarified. MicroRNAs (miRNAs) play an important role in the post-transcriptional regulation of the expression of drug transporters. Here, we investigated the intestinal expression profile of miRNAs after liver I/R and the role of miRNAs in the post-transcriptional regulation of P-gp in intestinal epithelial cells. Microarray analysis showed that microRNA-145 (miR-145) level was decreased in the small intestine of I/R rats. This downregulation of miR-145 was further confirmed by real-time polymerase chain reaction. In silico analysis revealed that 3′-untranslated regions (UTRs) of rat Mdr1a, mouse Mdr1a, and human MDR1 mRNA retain binding sites for miR-145. Luciferase assays using MDR1 3′-UTR reporter plasmid in HEK293 cells showed that luciferase activity was decreased by the overexpression of miR-145, and the deletion of miR-145 binding site within MDR1 3′-UTR abolished this decreased luciferase activity. The downregulation of miR-145 in Caco-2 cells, an epithelial cell line derived from human colon, increased P-gp expression and efflux activity of rhodamine 123, but not MDR1 mRNA level. These findings demonstrated that miR-145 negatively regulates the expression and function of P-gp through the repression of mRNA by direct interaction on the 3′-UTR of MDR1 mRNA. In addition, the downregulation of miR-145 should significantly contribute to the elevated intestinal P-gp expression after liver I/R. Our results provide new insight into the post-transcriptional regulation of intestinal P-gp. Copyright © 2013 by The American Society for Pharmacology and Experimental Therapeutics.

Tanaka T.,Tohkai Institute of Cytopathology | Tanaka T.,Gifu University | Tanaka T.,Kansai University of Health Sciences | Tanaka M.,Kinjo Gakuin University | Kuno T.,Gifu University
Journal of Biomedicine and Biotechnology | Year: 2012

β-Cryptoxanthin, a carotenoid, and hesperidin, a flavonoid, possess inhibitory effects on carcinogenesis in several tissues. We recently have prepared a pulp (CHRP) and citrus juices (MJ2 and MJ5) from a satsuma mandarin (Citrus unshiu Mar.) juice (MJ). They contain high amounts of β-cryptoxanthin and hesperidin. We have demonstrated that CHRP and/or MJs inhibit chemically induced rat colon, rat tongue, and mouse lung tumorigenesis. Gavage with CHRP resulted in an increase of activities of detoxifying enzymes in the liver, colon, and tongue rats'. CHRP and MJs were also able to suppress the expression of proinflammatory cytokines and inflammatory enzymes in the target tissues. This paper describes the findings of our in vivo preclinical experiments to develop a strategy for cancer chemoprevention of colon, tongue, and lung neoplasms by use of CHRP and MJs. © 2012 Takuji Tanaka et al.

Hayashi M.,Kinjo Gakuin University
Environment and Planning A | Year: 2014

After the 1970s the new urban poverty (NUP) ballooned in Japan and the US, and it evoked policy responses that produced new, rescaled regulatory spaces to contain the poor on the fringe of social rights and the capital circuit. The paper illuminates this process through the comparison of Japanese and US trajectories, both of which, evolving through economic crises, have established unique pathways. The author first constructs a theoretical framework based on Marxian, Polanyian, and Lefebvrean traditions. Then, he compares national-scale poverty regulation in Japan and the US from the 1950s through the 2000s. Lastly, the author examines how the countries' regulation of a major aspect of the NUP-homelessness-intensified multiscalar rescaling processes. The paper concludes that regulation of the NUP represents a significant instance of uneven spatial development of capitalism mediated by the state that requires synthetic research. © 2014 Pion and its Licensors.

Urban social movements (USMs) and regulation have co-evolved in Japan to deal with homelessness, spatializaing their politics on the national and subnational scales. The author first theorizes these USM-regulation relationships as scale-oriented dialectics between two opposing forces-"commoning and othering"-both of which in my view are always internalized in today's "rebel cities" (Harvey 2012, Rebel Cities, Verso). Then, he analyzes two trajectories of USMs that attempted commoning-ie radical opening up of public goods/spaces within "zones of weakness" (Lefebvre)-against policing and workfare disciplines. The author detects "rescaling" dialectics in the case of Yokohama and "nationalizing" dialectics in the case of Tokyo. Lastly, through exploring and refreshing Engels's notion of the (petit-)bourgeois utopia, the author concludes that our commoning projects and imaginaries are constrained by capitalist urban form that spatially others the homeless; but truly revolutionary moments of commoning emerge whenever people-even temporarily-conquer the fetishism of the public/private binary embedded in this urban form. © 2014 Antipode Foundation Ltd.

Inoue K.,Kinjo Gakuin University | Kitade M.,Kinjo Gakuin University | Hino T.,Kinjo Gakuin University | Oka H.,Kinjo Gakuin University
Food Chemistry | Year: 2011

Inhibition of angiotensin-converting enzyme (ACE) by various foods decreases the blood pressure. ACE inhibitors derived from natural components may be of therapeutic value in preventive medicine. In this study, we report a novel screening assay of ACE inhibitors from complex natural colourants and foods that employ solid phase extraction (SPE), high-throughput liquid chromatography (LC) separation, and stable isotope dilution electrospray tandem mass spectrometry (SID-ESI-MS/MS). When a target sample was subjected to N-Hippuryl-His-Leu (HHL) and ACE in phosphate buffer (pH 7.4), generated hippuric acid (HA) was extracted by SPE. LC/SID-ESI-MS/MS detection of HA allowed us to accurately identify the effects of complex substances such natural colourants and foods that inhibit the ACE of HHL. The major HA and HA-d 5 fragment ions at m/z 180 → 105 and 185 → 110 in the multiple reaction monitoring (MRM) mode can quantify levels that are lower than other methods. The LC/SID-ESI-MS/MS method described here is a rapid, selective, sensitive, and highly reproducible method for the determination of HA in various samples. Based on the assay developed, all samples such as natural colourants, infant formula, soy paste, ketchup, mayonnaise, wheat flour, orange juice, supplement drink, tea, and coffee could be accurately measured for ACE inhibition in various matrices. High-throughput LC/SID-ESI-MS/MS assay has no limitations in the evaluation of inhibition activity in various natural samples such as colour, high-matrix, and processed foods. © 2010 Elsevier Ltd. All rights reserved.

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