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Cumpanasoiu C.,King Michael I of Romania Banats University of Agricultural science | Necsulescu M.,Center for Medical Military Scientific Research Bucharest | Seres M.,King Michael I of Romania Banats University of Agricultural science | Liker M.,Victor Babes University of Medicine and Pharmacy Timisoara | And 3 more authors.
Romanian Biotechnological Letters | Year: 2014

This study aimed to characterize ten strains of Yersinia enterocolitica, a foodborn pathogen, using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF). Ten Yersinia enterocolitica pathogen strains were used to create an updated MALDI-TOF database: eight strains isolated from pigs' feces (YE 13258, YE 13469, YE 13249, YE RO 74, YE RO 86, YE VL1207, YE VL0108, and YE VL0408) and two standard strains (YE 13250 and YE 909574). Each strain was inoculated both on nutrient agar and blood agar plates, and from each culture three colonies were harvested and processed for extraction of bacterial 16S ribosomal proteins. MALDI-TOF measurements were carried out with Microflex LT 20 (Bruker Daltonik, Germany). Mass spectra acquired were analyzed and compared with each other and with equipment database. 16S ribosomal protein spectra analysis revealed a mass domain range between 800 and 12,000 Da and the presence of three mass peaks in all strains, classified in the following mass domains: 4828-4872 Da, 6227-6474 Da, and 9659-9673 Da. It may be concluded that all ten strains are very similar in terms of 16S ribosomal proteins structure, which demonstrate a high degree of relatedness, respectively the strains analyzed belongs to Yersinia enterocolitica species. © 2014 University of Bucharest. Source


Tirziu E.,King Michael I of Romania Banats University of Agricultural science | Cumpanasoiu C.,King Michael I of Romania Banats University of Agricultural science | Nichita I.,King Michael I of Romania Banats University of Agricultural science | Reman G.D.,University of Bucharest | And 2 more authors.
Romanian Biotechnological Letters | Year: 2014

The aim of the present paper was the comparative assessment of two ELISA tests on serum and milk samples and agar gel immunodiffusion test (AGID) regarding ability to detect enzootic bovine leukosis (EBL) in Timis County, according to farm type, age, breed and stage of lactation, and how these parameters affect the humoral immune response of positive cattle. Serum samples were analyzed using blocking ELISA and AGID, and the milk samples by indirect ELISA, calculating quality parameters of applied tests. The obtained results showed that AGID detected 43 positive cattle and the two ELISAs 51 positive animals. Performances of AGID were influenced by farm type, age and serum antibody level. It was established that cattle aged 3-6 years were most prone to develop EBL. The level of serum antibodies has been influenced by farm type, age and lactation phase, and milk antibodies level strictly by the phase of lactation; the lowest values of milk antibodies were registered between 2nd and 8th month of lactation. Consumption of insufficiently heat-treated milk from BLV positive cattle in 2-8 months of lactation is associated with increased risk of infection in humans. © 2014 University of Bucharest. Source

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