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El-Kasheif M.A.,National Institute of Oceanography and Fisheries of Egypt | Authman M.M.N.,National Research Center of Egypt | Al-Ghamdi F.A.,King Abd El Aziz University | Ibrahim S.A.,Benha University | El-Far A.M.,National Institute of Oceanography and Fisheries of Egypt
Journal of Fisheries and Aquatic Science | Year: 2015

In the present investigation, age, growth, mortality, exploitation, yield and biomass and reproduction of Nile tilapia (Oreochromis niloticus) from El-Bahr El-Faraouny Canal, Al-Minufiya Province, Egypt, were determined. The regression parameter “n” of dependence of total weight upon total length was found to be negative allometric growth. The coefficients of condition were computed. The annual rings on fish scales were used for age determination. The longevity of this species was found to be 6 years. The maximum increase in length was noted during the first year of life and showing a gradual decrease in growth as the fish got older. The O. niloticus population had a sex ratio of 1:1.08 (M:F), which did not deviates (χ2 = 1.997, p>0.05) from the hypothetical distribution of 1:1. The maturity stages were classified macroscopically into five stages. Higher values of gonadosomatic index (GSI) for males and females occurred nearly throughout the year with peak from May to August indicating prolonged spawning season. There is a selective mortality towards smaller fish sizes. Therefore, for management purposes it is recommended that the local authorities must be set a regulation to control the illegal fishing activity and larger mesh sizes of fishing nets should be used to increase the mean lengths at first capture and their marketable sizes leading to more economic returns and also to conserve the spawning stock part of O. niloticus population. A comparison of the various parameters of O. niloticus in the present study with those of other authors in different localities and times in Egypt revealed year-to-year and geographically differences. These differences were discussed and were found to be attributed to the effect of increased pollution of the canal. Finally, the present study recommended that El-Bahr El-Faraouny Canal must be in protection against pollution. © 2015 Academic Journals Inc.

Sahab A.F.,National Research Center of Egypt | Nawar L.S.,King Abd El Aziz University
International Journal of ChemTech Research | Year: 2015

The aim of the current study were to determine the quality of Moringa oleifera seeds and to detect the in-vitro antifungal activity of seed extracts and their phytochemical and elemental composition. Seeds of moringa recently collected or stored for one to three years were contaminated with fungi with an average of 48.44% on PDA medium. The percentage of fungal infection was higher in seeds produced during season of 2011(stored for 3 years), followed by seeds produced at season of 2012 with significant difference (87.50 and 50.00% respectively). Moringa seeds recently collected during season of 2014 showed low fungal densities than the corresponding figures of stored seeds for 1-3 years. Fourteen species which belong to nine genera were detected and they were classified as, Alternaria alternata (11.82%), Aspergillus candidus (4.32%), A. flavus (6.34%), A. niger (14.46%), A. regulosus (1.87%), A. sydowi (3.08%), A. terreus (6.09%), Chaetomium globosum (7.55%), Fusarium solani (2.35%), Helminthosporium sp. (5.97%), Macrophomina phaseolina (6.88), Nigrospora sphaericica (16.69%), Rhizoctonia solani (4.19%) and Rhizopus nigricans (8.35). The ethanolic seed extract was found to be more effective than the water extract. The ethanolic seed extract at 100% cause increase in zone of inhibition in all tested fungi reaching 2.70-3.77mm, whereas, at 50% concentration reaching between 1.40-1.93mm. The aqueous extract of moringa seeds at 100% concentration showed moderate activity against all tested fungi (inhibition zone between 0.43-1.33 mm) compared with the standard fungicide (inhibition zone 4.23 - 4.90 mm). The results of the phytochemical analysis revealed differences in the presence of components among the seed extracts. The total phenol was decreased from 7.00 to 3.19 mg/g (54.4% decrease) and total flavonoids from 0.697 to 0.550 mg/g (21.09 decrease) in moringa recently collected (2014) and stored seeds for 3 years respectively. The percentage of nitrogen, phosphorus and potassium were high in recently collected seeds than in the stored seeds, as the percentages were 5.6, 0.64 and 1.18 % in recently collected seeds respectively and reduced to 5.1, 0.91 and 1.11 % respectively in old seeds for 3 years. GC/MS analysis of recent collected moringa and stored seed extracts led to the identification of different compounds. Extract of recent collected seeds during season 2014 contained some compounds varied in their chemical composition and molecular weight than old seeds of 3 years. © 2015, Sphinx Knowledge House. All rights reserved.

El-Gammal O.A.,Mansoura University | Elmorsy E.A.,King Abd El Aziz University | Elmorsy E.A.,Mansoura University | Sherif Y.E.,Mansoura University | Sherif Y.E.,Taibah University
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2014

New Cu(II) and Zn(II) complexes of 2-(naphthalen-1-yloxy)-N′-(1- (pyridin-2-yl)ethylidene) acetohydrazide (HA2PNA) have been prepared and characterized by elemental analyses, spectral (IR, UV-visible, ESR and 1H NMR) as well as magnetic and thermal measurements. According to the data, the complexes assigned the formulae: [Cu(A2PNA)2]H 2O and [Zn(A2PNA)(OAc)(H2O)], respectively. IR data revealed that the ligand acts as before ONN and after morever ONN mononegative tridentate via deprotonated carbonyl oxygen (CO) and both (CN)imine and (CN)pyridine nitrogen atoms. The bond lengths, bond angles, HOMO, LUMO, dipole moment and charges on the atoms have been calculated by using density functional theory (DFT) at B3LYP level with 6-31G and 6-31G(d,p) basis sets to confirm the geometry of the ligand and the investigated complexes. Also, the kinetic parameters were determined for each thermal degradation stage of the complexes using Coats-Redfern and Horowitz-Metzger methods. Moreover, the complexes have been tested for anti-inflammatory and analgesic activity in rat model of collagen adjuvant arthritis and compared with piroxicam. All the compounds showed a significant anti-inflammatory and analgesic effect versus piroxicam. © 2013 Elsevier Ltd. All rights reserved.

Al-Mhyawi S.R.,King College | Al-Mhyawi S.R.,King Abd El Aziz University
Oriental Journal of Chemistry | Year: 2014

The inhibition efficiency of extract of Garlic on aluminium in hydrochloric acid solutions has been evaluated by weight loss techniques. Values of inhibition efficiency obtained are dependent upon the concentration of inhibitor and temperature. Generally, inhibition was found to increase with inhibitor concentration, half-life, activation energy but decrease with temperature and firstorder rate constant at the temperatures studied. Physical adsorption mechanism has been proposed for the inhibition and Langmuir , Temkin adsorption isotherm was obeyed. Garlic is an inhibitor of aluminium corrosion in 0.5 M hydrochloric acid solution.The values of standard free energy of adsorption suggest that the adsorption of inhibitor on aluminium surface occurred by physisorption mechanism. the negative sign of the Free Energy of adsorption indicates that the adsorption of the inhibitors on the aluminum surface was a spontaneous process.the negative values of enthalpy of adsorption ("H) suggest that the chemical reaction involved in the adsorption of the inhibitors on the metal surface is an exothermic process, hence increase in the reaction temperature of the medium will decrease the inhibition efficiency.

Hegazy W.K.E.-L.,National Research Center of Egypt | Tork S.E.A.,King Abd El Aziz University | Abdel-Salam M.S.,National Research Center of Egypt
Journal of Applied Biological Sciences | Year: 2012

This work aimed to characterize recombinant strains, with a high production level of L-asparaginase, previously obtained by protoplast fusion between Bacillus subtilis and Bacillus cereus. The genetic relationship between fusants and their parents was determined using a simple PCRrestriction fragment length polymorphism (RFLP) of 16S rDNAs -based method, and by analyzing the specific L-asparaginase genes and protein patterns. Our results indicate that all fusants have morphological characteristics and specific L-asparaginase genes similar to the Bacillus cereus strain whereas protein pattern and 16S rDNAs -RFLP analysis show mixed characteristics from both parents. Different genetic relationships were found between each fusant and its parental strain. These results confirm that protoplast fusion is an important technique in strain improvement. It was used to combine genes from different organisms for creating strains with desired properties.

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