Kikkoman Corporation

Noda, Japan

Kikkoman Corporation

Noda, Japan

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To provide: a seed koji (Aspergillus starter) for brewing that either does not includes any food items containing allergenic substances as the main raw material and auxiliary raw material or includes only up to an amount that does not induce an allergy in order to prevent carryover of allergenic substances derived not only from the main raw material but also auxiliary raw materials and by which it is possible to produce a soy sauce-like seasoning having a similar flavor to that of soy sauce; and koji for brewing that uses the seed koji for brewing; as well as a soy sauce-like seasoning that uses the koji for brewing. The present problems are resolved by a seed koji for brewing that is obtained by inoculating a koji mold belonging to the Aspergillus genus into a seed koji raw material, where the seed koji raw material is a pea, koji for brewing that is obtained by using the seed koji for brewing, as well as a soy sauce-like seasoning that is obtained by using the koji for brewing.


Patent
Kikkoman Corporation | Date: 2017-01-04

The present invention provides a flavin-binding glucose dehydrogenase that has one or more amino acid substitutions at positions corresponding to position 88 or position 554 in the amino acid sequence set forth in SEQ ID NO: 1.


A seed koji (Aspergillus starter) for brewing is provided. The seed koji is obtained by inoculating koji mold(s) belonging to an Aspergillus genus into a seed koji raw material, where the seed koji raw material is a pea.


Provided are: a citrus seed extract-containing composition, a food and a drug all of which sufficiently exert a novel function of a citrus (specifically, a citrus seed extract), and a method for producing the citrus seed extract-containing composition. The citrus seed extract-containing composition includes obacunone and nomilin both derived from a citrus seed extract. Herein, the ratio of the obacunone content to the nomilin content (i.e., obacunone content/nomilin content) is at least 0.020.


Patent
Kikkoman Corporation | Date: 2015-10-14

The object of the present invention is to provide a soy sauce with a pH of less than 4.6, which has reduced acidic taste and acidic odor and improved umami in the aftertaste. The soy sauce with a pH of less than 4.6, which has reduced acidic taste and acidic odor and improved umami in the aftertaste can be obtained by adjusting the ethyl lactate concentration per 1 w/v % of total nitrogen concentration in the soy sauce to 20 ppm or more.


Provided are: a citrus seed extract-containing composition, a food and a drug all of which sufficiently exert a novel function of a citrus (specifically, a citrus seed extract), and a method for producing the citrus seed extract-containing composition. The citrus seed extract-containing composition includes obacunone and nomilin both derived from a citrus seed extract. Herein, the ratio of the obacunone content to the nomilin content (i.e., obacunone content/nomilin content) is at least 0.020.


Patent
Kikkoman Corporation | Date: 2016-08-31

This invention provides an amadoriase that acts on the -chain of hemoglobin A1c (HbA1c) and generates hydrogen peroxide, a method for measurement of HbA1c using such amadoriase, and a reagent kit for measurement of HbAlc using such amadoriase. The method for measurement of HbAlc involves the use of an amadoriase that has specific activity of 0.1 U/mg or greater to F6P and oxidizes the HbA1c -chain amino terminus so as to generate hydrogen peroxide, and the reagent kit for measurement of HbA1c comprises such amadoriase. The method and the kit for measurement of HbAlc enable quantification of HbAlc to be performed rapidly, simply, and accurately.


Patent
Kikkoman Corporation | Date: 2016-03-09

[Problem to Be Solved] A present invention provides a soy milk fermented substance with a taste and flavor or smooth physicality similar to that of yogurt obtained by fermenting milk with a lactic acid without applying processing treatment such as enzyme treatment or extraction treatment to soy milk which is a material and without adding a material such as a sugar source, even in a case where only Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus are employed as a lactic acid bacteria mixture starter at the time of making a fermented substance which employs soy milk as a material. [Means for Solving the Problem] The problem is solved by a soy milk fermented substance obtained by using a lactic acid bacteria mixture starter including: Streptococcus thermophilus which is capable of accumulating 0.4 g/L or more of fructose in the fermented substance when inoculated and cultured in soy milk; and Lactobacillus debrueckii subsp. bulgaricus which is capable of accumulating 0.4 g/L of D-lactic acid in the fermented substance when inoculated and cultured in soy milk.


Patent
Kikkoman Corporation | Date: 2016-08-31

This invention provides an amadoriase that can react with a wide variety of glycated peptides generated upon hydrolysis of the chain of hemoglobin A1c (HbA1c) to generate hydrogen peroxide, a method for measurement of HbAlc using such amadoriase, and a reagent kit for measurement of HbAlc using such amadoriase. Provided is an amadoriase obtained by substitution of one or more amino acids at positions corresponding to amino acids selected from the group consisting of amino acids 62, 63, 102, 106, 110, 113, and 355 in the amino acid sequence of the amadoriase derived from the genus Coniochaeta or the like which amadoriase is capable of oxidizing a wide variety of glycated peptides to generate hydrogen peroxide. Further provided is a method for measurement of HbAlc comprising using such amadorise as well as a reagent kit for measurement of HbAlc comprising such amadoriase. This invention can provide a method for measurement of HbAlc that enables quantification of HbAlc to be performed rapidly, simply, and accurately with the use of a small amount of a protease and a kit used for such measurement. This invention can also provide a method for measurement of HbAlc that enables quantification of HbAlc to be performed rapidly, simply, and accurately, with high sensitivity with the use of a small amount of a protease and a kit used for such measurement.


Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.

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