Hara N.,Niigata University |
Saito H.,Kido Hospital |
Takahashi K.,Niigata University |
Takeda M.,Yamanashi University
International Journal of Urology | Year: 2013
Objectives: To clarify the prevalence of lower urinary tract symptoms and overactive bladder in patients with chronic methyl mercury poisoning. Methods: A total of 151 patients (61 men and 90 women; mean age 72.1 years) with Niigata Minamata disease were enrolled. An age- and sex-matched group of 150 participants was used as control. Patients reported their International Prostate Symptom Score and overactive bladder symptom score. Results: In men, the total, storage and voiding International Prostate Symptom Score scores were higher in the Niigata Minamata disease group than in the control group (10.6±7.8 vs 5.0±5.0, 4.5±3.3 vs 2.4±2.4 and 6.1±5.1 vs 2.7±3.1, respectively, P<0.001 in all). In women, these scores were also higher in the Niigata Minamata disease group than in the control group (8.9±7.3 vs 4.0±4.0, 4.4±3.2 vs 2.8±2.4 and 4.5±5.0 vs 1.3±2.0, respectively, P<0.001 in all). The prevalence of overactive bladder was more frequent in the Niigata Minamata disease group compared with that in the control group (51.7% vs 26.7%, P<0.001). In both men and women, the overactive bladder symptom score was higher in the Niigata Minamata disease group than in the control group (4.1±3.0 vs 2.4±2.9, P=0.002 and 4.6±3.6 vs 2.7±2.9, P<0.001, respectively). The International Prostate Symptom Score and overactive bladder symptom score in the Niigata Minamata disease group were highest in patients aged 60-69 years (P<0.001 in both), whereas these increased in an age-dependent manner in the control group. Conclusions: Lower urinary tract symptoms and overactive bladder are severe and highly prevalent conditions among patients with methyl mercury poisoning. The higher prevalence of lower urinary tract symptoms among patients aged 60-69 years might be related to the fact that they were exposed to methyl mercury during their childhood/development. © 2012 The Japanese Urological Association.
Maruyama K.,Niigata Seiryo University |
Yorifuji T.,Okayama University of Science |
Tsuda T.,Okayama University of Science |
Sekikawa T.,Nuttari Clinic |
And 2 more authors.
Journal of Biomedicine and Biotechnology | Year: 2012
Background. Large-scale poisonings caused by methyl mercury (MeHg) have occurred in Japan (Minamata in the 1950s and Niigata in the 1960s) and Iraq (in the 1970s). The current WHO neurological risk standard for adult exposure (hair level: 50g/g) was based partly on evidence from Niigata which did not consider any cases who were diagnosed later and/or exposed to low level of MeHg (hair mercury level less than 50g/g). Methods. Early in the Niigata epidemic in June 1965 there were two extensive surveys. From these two surveys, we examined 103 adults with hair mercury measurement who consulted two medical institutions. We compared the prevalence and the distribution of neurological signs related to MeHg poisoning between exposure categories. Result. We found 48 subjects with neurological signs related to MeHg poisoning who had hair mercury concentration less than 50g/g. Among the neurological signs, sensory disturbance of the bilateral distal extremities was observed more frequently, followed by disequilibrium, hearing impairment, and ataxia, in groups with hair MeHg concentration both below 50g/g and over 50g/g. Conclusion. The present study suggests the possibility that exposure to MeHg at levels below the current WHO limits could cause neurologic signs, in particular, sensory disturbance. © Copyright 2012 Kimio Maruyama et al.
Abe T.,Niigata University |
Aoki T.,Niigata University |
Yata S.,Kido Hospital |
Okada M.,Niigata University
Atherosclerosis | Year: 2011
Background: Previous studies have indicated that sleep duration is associated with total mortality in a U-shaped fashion. The purpose of the current study was to examine the relationship between self-reported sleep duration and carotid artery atherosclerosis in a Japanese population. Methods: In 2009-2010, a total of 2498 participants (1195 men, 1303 women; age range, 23-92. years) were recruited from members of a Japanese community receiving annual health check-up at a local health center who agreed to participate in the study. Exclusion criteria were as follows: age <40 or ≥85. years; and more than one missing value from either laboratory data or questionnaire responses. A total of 2214 participants were entered into the study. Carotid artery arteriosclerosis was evaluated ultrasonographically and quantified as intima-medial thickness (IMT). The presence of carotid artery atherosclerosis was defined as IMT ≥ 1.2. mm. Sleep durations were compared with IMT measurements after controlling for confounding factors such as age, sex, lipid profile, fasting plasma glucose, hemoglobin A1c, blood pressure, alcohol intake, and smoking habit. Results: Sleep duration ≥7. h correlated significantly with the incidence of IMT ≥ 1.2. mm when compared with a sleep duration of 6. h (multivariate-adjusted odds ratio, 1.263; 95% confidence interval, 1.031-1.546, P = 0.024). Shorter sleep duration ≤5. h did not correlate significantly with the risk compared with a sleep duration of 6. h. Conclusion: Long sleep duration (≥7. h) correlated significantly with the incidence of carotid artery atherosclerosis compared with a sleep duration of 6. h, but shorter sleep duration did not. © 2011 Elsevier Ireland Ltd.
Ibusuki A.,Kagoshima University |
Kawai K.,Kagoshima University |
Kawai K.,Kido Hospital |
Yoshida S.,Hokkaido University |
And 8 more authors.
Journal of Investigative Dermatology | Year: 2014
Murine epidermal γδ T cells, known as dendritic epidermal T cells (DETCs), survey tissue stress through the invariant T-cell receptor (TCR) and non-clonotypic receptors such as NKG2D. NKG2D signaling via the DAP10-phosphatidylinositol 3-kinase (PI3K) pathway directly stimulates cytotoxicity in natural killer (NK) cells and costimulates CD8 + T cells to augment TCR signals. In activated murine NK cells, NKG2D signals also via the DAP12-Syk/ZAP70 pathway that triggers both cytotoxicity and cytokine production. It remains controversial whether NKG2D on DETCs is a primary activating receptor or functions only as a costimulatory receptor, and signaling pathways initiated by NKG2D ligation in DETCs have not been analyzed. We show that stimulation of short-term DETC lines with recombinant NKG2D ligands triggers degranulation (exocytosis of cytotoxic granules) via the PI3K-dependent signaling pathway, but does not induce cytokine production or Syk/ZAP70 activation. Coengagement of TCR or Syk/ZAP70 signaling was not crucial for DETC-mediated killing of NKG2D ligand-expressing target cells. Thus, NKG2D can function as a coactivating stress receptor that directly triggers cytotoxicity in DETCs, at least after priming, via the PI3K-dependent, Syk/ZAP70-independent signaling pathway. © 2014 The Society for Investigative Dermatology.
Abe T.,Niigata University |
Tsuda A.,Kido Hospital |
Yata S.,Kido Hospital |
Matsuto T.,Niigata University |
Okada M.,Niigata University
Annals of Clinical Biochemistry | Year: 2010
Background: Great differences in age-standardized mortality rates by cardiovascular disease exist among countries. We prospectively assessed determinants of future cardiovascular changes in a Japanese cohort. Methods: In 1996, 1011 men and 1153 women from a Japanese community participated in a study on cardiovascular risk factors at a local health centre. Of these, the 896 subjects who visited the centre both in 1996 and 2001 were selected for the analysis. The presence of cardiovascular changes was defined as the appearance of one or more of the following in five years: positive electrocardiographic findings, intima-media thickness of the carotid artery ≥0.8 mm and retinal vascular changes ≥Keith-Wegener-Barker classification stage I. Results: Of the 607 subjects who had no history of cardiovascular disease in 1996, 421 showed changes in 2001. Both the age-adjusted and multivariate models showed that the risk of the cardiovascular changes increased as systolic blood pressure (SBP) increased to ≥135 mmHg (multivariate odds ratio = 1.739, 95% confidence interval = 1.076-2.810, P < 0.05) compared with those with an SBP of 110-134 mmHg. When we made the analyses only for laboratory test results by excluding SBP, body mass index, alcohol intake and current smoking from the regression model, high-density lipoprotein cholesterol and fasting plasma glucose were significant variables. Conclusion: The risk of future cardiovascular changes is significantly greater with higher SBP in the Japanese population.
Hokari S.,Niigata University |
Tsukada H.,Niigata City General Hospital |
Ito K.,Kido Hospital |
Shibuya H.,Niigata City General Hospital
Internal Medicine | Year: 2010
A 58-year-old woman with an 18-year history of primary biliary cirrhosis was admitted because of pneumococcal pneumonia. She was treated with antibiotics and mechanical ventilation. After the pneumonia improved, she developed severe watery diarrhea. Although vancomycin was administered enterally, the diarrhea persisted. She died of multiple organ failure within 16 days of the onset of diarrhea. An autopsy showed intracapillary cryptococci in the systemic organs, especially in the intestinal tract. The cause of diarrhea was considered to be extensive intestinal mucosal necrosis due to disseminated cryptococcosis. This is a rare case of cryptococcal infection manifesting as acute diarrhea. © 2010 The Japanese Society of Internal Medicine.
Horiuchi Y.,Juntendo University |
Hirayama S.,Juntendo University |
Soda S.,Niigata City General Hospital |
Seino U.,Niigata University |
And 7 more authors.
Journal of Atherosclerosis and Thrombosis | Year: 2010
Aim: Hypercholesterolemic patients with inflammation are at high risk for cardiovascular events. Statins exert anti-inflammatory action independent of their cholesterol-lowering action. This study sought to clarify whether statin therapy reduces inflammatory markers in hypercholesterolemic patients and to determine factors that predict the reduction in these markers. Methods: Fasting concentrations of lipoproteins and inflammatory markers were measured in 54 hypercholesterolemic patients, and age- and gender-matched healthy volunteers. Carotid atherosclerosis was determined by ultrasonography. Blood samples were also analyzed in hypercholesterolemic patients after 4 weeks of statin therapy. Results: The high-sensitivity C-reactive protein (hs-CRP) and serum amyloid A (SAA) protein concentrations did not differ between the two groups. Statin therapy reduced the median hs-CRP and SAA concentrations in hypercholesterolemic patients from 0.75 to 0.60 mg/L (p=0.05), and from 3.95 to 3.20 μg/mL (p=0.20), respectively. These reductions were significant for both markers, but only in subgroups with high baseline concentrations. Statins exhibited different results for hs-CRP and SAA in the presence of carotid atherosclerosis. Conclusions: Statin therapy reduces inflammatory markers in hypercholesterolemic patients, and this anti-inflammatory action is limited to patients whose inflammatory markers are elevated at baseline.
Yamaguchi K.,Niigata University |
Hama H.,Kido Hospital
Endocrine Journal | Year: 2011
The anteroventral third ventricular region (AV3V) is a pivotal area for osmotic responses and integration of autonomic functions. The purpose of this study was to investigate whether the gamma-aminobutyric acid (GABA)-ergic activity in the AV3V may be involved in the regulation of arginine vasopressin (AVP) secretion and related phenomena under the conditions with or without hypovolemia. Experiments were performed in conscious rats. We found that AV3V infusion with the GABA A receptor antagonist bicuculline in euvolemic rats caused prompt increases in plasma AVP, osmolality, glucose, arterial pressure and heart rate. The effects of the bicuculline infusion were abolished by prior infusion of a GABA A receptor agonist, muscimol. When repeated twice with a 10-min interval, removal of systemic blood (10 mL/ kg body weight) lowered arterial pressure and enhanced plasma AVP, osmolality, glucose and angiotensin II. Muscimol infusion in the AV3V, but not in the cerebral ventricle, inhibited the responses of plasma AVP and glucose, despite having no effect in a sham hemorrhagic state. The inhibition of the AVP response by the muscimol infusion was also verified in rats given a combined stimulus of bleeding plus an osmotic load. In contrast, AV3V infusion with the GABA B receptor agonist baclofen tended to intensify the hemorrhagic responses of plasma AVP and glucose, despite its potency to prevent the hemorrhagic fall in arterial pressure. These results, taken together with our previous data, suggest that hypovolemic stimuli, like hyperosmotic stimuli, may promote AVP secretion by causing the inhibition of AV3V GABA A-ergic activity responsible for potentiation of glutamatergic activity. © The Japan Endocrine Society.
Nakamizo S.,Kyoto University |
Egawa G.,Kyoto University |
Tomura M.,Kyoto University |
Sakai S.,Kyoto University |
And 14 more authors.
Journal of Investigative Dermatology | Year: 2015
A large number of gamma delta T cells (γδ T cells) are located within epithelial tissues including the skin. In mice, epidermal and dermal γδ T cells consist of distinct subsets and have specific roles in cutaneous immune responses. A recent study demonstrated that γδ T cells and cutaneous dendritic cells migrate from the skin to the draining lymph nodes (LNs). However, it remains unclear whether they regulate the antigen-specific immune response within the LNs. Herein, we investigated their properties and role in the LNs using the Mycobacterium bovis bacille Calmette-Guérin (BCG) infection model. In vivo cell labeling analysis revealed that most of the migratory subset comprised dermal Vγ4+ cells. This population transmigrated from the skin to the LNs in a Gi-coupled chemokine receptor-independent manner. By depleting Vγ4+ cells, the intranodal expansion of CD8+ T cell against BCG was significantly attenuated. In addition, in vitro analysis revealed that Vγ4+ cells produced TNF- and enhanced IL-12 production by dendritic cells. Taken together, these findings suggest that dermal Vγ4+ cells are a unique subset that possesses a migratory potency to the skin-draining LNs and enhances the dendritic cell function therein. © 2015 The Society for Investigative Dermatology.
PubMed | Kumamoto University, Japan Science and Technology Agency, Kyoto University, Kyushu University and Kido Hospital
Type: Journal Article | Journal: The Journal of investigative dermatology | Year: 2015
A large number of gamma delta T cells ( T cells) are located within epithelial tissues including the skin. In mice, epidermal and dermal T cells consist of distinct subsets and have specific roles in cutaneous immune responses. A recent study demonstrated that T cells and cutaneous dendritic cells migrate from the skin to the draining lymph nodes (LNs). However, it remains unclear whether they regulate the antigen-specific immune response within the LNs. Herein, we investigated their properties and role in the LNs using the Mycobacterium bovis bacille Calmette-Gurin (BCG) infection model. In vivo cell labeling analysis revealed that most of the migratory subset comprised dermal V4(+) cells. This population transmigrated from the skin to the LNs in a Gi-coupled chemokine receptor-independent manner. By depleting V4(+) cells, the intranodal expansion of CD8(+) T cell against BCG was significantly attenuated. In addition, in vitro analysis revealed that V4(+) cells produced TNF- and enhanced IL-12 production by dendritic cells. Taken together, these findings suggest that dermal V4(+) cells are a unique subset that possesses a migratory potency to the skin-draining LNs and enhances the dendritic cell function therein.