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Naderi M.,Khorramshahr Marine Science and Technology University | Wong M.Y.L.,University of Wollongong | Gholami F.,Islamic Azad University at Yasuj
Aquatic Toxicology | Year: 2014

In the recent years, there has been a growing concern about the production and use of bisphenol-A substitute, namely bisphenol-S (BPS). Due to its novel nature, there have been few studies addressing the ability of BPS to disrupt the endocrine system of animals. In the present study, zebrafish (Danio rerio) embryos were exposed to and reared in various concentrations of BPS (0, 0.1, 1, 10 and 100. μg/l) for 75 days. Then adult males and females were paired in spawning tanks for 7 days in clean water and the consequent effects on fish development, reproduction, plasma vitellogenin (VTG), sex steroids and thyroid hormone levels were investigated as endpoints. After 75 days of exposure, there was a skewed sex ratio in favor of females. The results also showed that body length and weight significantly decreased in males exposed to 100. μg/l of BPS. Gonadosomatic index was significantly reduced in fish at ≥10. μg/l. Hepatosomatic index exhibited a significant increase in both male and female fish. At ≥1. μg/l of BPS, plasma 17β-estradiol levels were significantly increased in both males and females. However, plasma testosterone showed a significant reduction in males exposed to 10 and 100. μg/l of BPS. A significant induction in plasma VTG level was observed in both males and females at ≥10. μg/l of BPS. Plasma thyroxine and triiodothyronine levels were significantly decreased at 10 and 100. μg/l of BPS in males, and at 100. μg/l in females. Egg production and sperm count were also significantly decreased in groups received 10 and 100. μg/l of BPS. Moreover, once time to hatching and hatching rates were calculated for fertilized eggs the postponed and decreased rates of hatching were observed. Taken together, these results suggest that developmental exposure to low concentrations of BPS has adverse effects on different parts of the endocrine system in zebrafish. © 2014.


Hedayati A.,Gorgan University of Agricultural Sciences and Natural Resources | Safahieh A.,Khorramshahr Marine Science and Technology University
Toxicology and Industrial Health | Year: 2012

This study was undertaken in two separate environmental and experimental conditions. The environmental test was carried out at creeks in the Persian Gulf and the experimental test was undertaken in seawater re-circulatory tanks. Mercury concentrations were determined using a standard cold vapour atomic absorption method. Serum enzymes were determined with Diagnostics Infinity reagent kit. Serum glucose and total protein were measured photometrically. Serum hormones were assayed using diagnostic ELISA kits. Results of the present investigation indicated that the sub-acute and chronic mercury concentrations tested may cause several changes in the biochemical and hormone parameters of the studied fish and we can use these changes as biomarkers for mercury detection. The range of mercury concentrations found in the creek water and specially sediments along the Mahshahr coast was higher than the other marine environment. It was declared that between enzymatic indices serum acid phosphatase (ACP) amount and ACP activity, between hormonal indices testosterone, T3 and T3/T4 and between metabolite index total protein can be considered as suitable and effective biomarkers of mercury pollution in yellowfin seabream. © 2011 The Author(s).


Keyvanshokooh S.,Khorramshahr Marine Science and Technology University | Gharaei A.,University of Zabol
Aquaculture Research | Year: 2010

The availability of monosex populations of caviar-producing female sturgeon would considerably enhance the economic viability of domestic caviar production systems. However, it is not possible to distinguish males from females by morphological characters at larval, juvenile and even adult stages. The mechanism of sex determination in sturgeons is poorly understood, and to date no sex-specific markers in sturgeon have been reported. This review concentrates on the methodologies used to elucidate the mode of sex determination in sturgeon species and provides information on the molecular tools used to determine genetic sex markers. © 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd.


Azarm H.M.,Khorramshahr Marine Science and Technology University | Lee S.-M.,Gangneung - Wonju National University
Aquaculture Research | Year: 2014

A feeding trial was conducted to evaluate the effect of replacing fish meal protein with fermented soybean meal (FSM) on the growth performance, feed utilization, amino acid profile, body composition, morphological parameters, activity of antioxidant and digestive enzymes of black sea bream (Acanthopagrus schlegeli) juvenile. Five isonitrogenic and isolipidic diets were prepared with levels of 0 (control), 80, 160, 240 and 320 g kg-1 FSM. Triplicate groups (40 fish per tank) of juvenile black sea bream with initial weight of 1.17 ± 0.04 g were hand-fed to visual satiation at three meals per day for 8 weeks. The fish fed diets containing different levels of FSM had no significant differences regarding survival and specific growth rate compared with control group. Feed and protein efficiency ratios of fish fed diet containing 320 g kg-1 FSM were significantly lower than those of control group. Daily feed intake and daily protein intake of fish fed diet containing 240-320 g kg-1 were significantly higher than those of control group. Hepatosomatic index and condition factor of fish were not affected by different dietary FSM level. Fish fed diets containing 240-320 g kg-1 FSM had significantly higher visceral somatic index than control group. Whole body proximate and amino acid compositions of fish were not affected by dietary FSM level. The activity of digestive enzymes in the intestine was not affected by dietary FSM level. The activity of glutathione peroxidase in liver was significantly higher for fish fed the diet containing 160 g kg-1 FSM compared with control group. This study showed that up to 40% fish meal in the diets of juvenile black sea bream could be replaced by fermented soybean meal with supplementation of methionine, lysine and taurine. © 2012 John Wiley & Sons Ltd.


Khalili-Fard V.,Khorramshahr Marine Science and Technology University | Ghanemi K.,Khorramshahr Marine Science and Technology University | Nikpour Y.,Khorramshahr Marine Science and Technology University | Fallah-Mehrjardi M.,Khorramshahr Marine Science and Technology University
Analytica Chimica Acta | Year: 2012

The application of sulfur microparticles as efficient adsorbents for the solid-phase extraction (SPE) and determination of trace amounts of 10 polycyclic aromatic hydrocarbons (PAHs) was investigated in sea water and wastewater samples using high performance liquid chromatography coupled with an ultraviolet detector (HPLC-UV). Parameters influencing the preconcentration of PAHs such as the amount of sulfur, solution flow rate and volume, elution solvent, type and concentration of organic modifier, and salt effect were examined. The results showed that at a flow rate of 10mLmin -1 for the sample solutions (100mL), the PAHs could be adsorbed on the sulfur microparticles and then eluted by 2.0mL of acetonitrile. For HPLC-UV analysis of extracted PAHs, the calibration curves were linear in the range of 0.05-80.0μgL -1; the coefficients of determinations (r 2) were between 0.9934 and 0.9995. The relative standard deviations (RSDs) for eight replicates at two concentration levels (0.5 and 4.0μgL -1) of PAHs were lower than 7.3%, under optimized conditions. The limits of detection (LODs, S/N=3) of the proposed method for the studied PAHs were 0.007-0.048μgL -1. The recoveries of spiked PAHs (0.5 and 4μgL -1) in the wastewater and sea water samples ranged from 78% to 108%. The simplicity of experimental procedure, high extraction efficiency, short sample analysis, and using of low cost sorbent demonstrate the potential of this approach for routine trace PAH analysis in water and wastewater samples. © 2011 Elsevier B.V.


Karimi-Maleh H.,Kerman Graduate University of Technology | Biparva P.,Sari University of Agricultural Sciences and Natural Resources | Hatami M.,Khorramshahr Marine Science and Technology University
Biosensors and Bioelectronics | Year: 2013

A carbon paste electrode (CPE) modified with (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol (DEDE) and NiO/CNTs nanocomposite was used for the sensitive voltammetric determination of cysteamine (CA), nicotinamide adenine dinucleotide (NADH) and folic acid (FA) for the first time. The synthesized materials were characterized with different methods such as XRD, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The modified electrode exhibited a potent and persistent electron mediating behavior followed by well-separated oxidation peaks of CA, NADH and FA. The peak currents were linearly dependent on CA, NADH and FA concentrations using square wave voltammetry (SWV) method in the ranges of 0.01-250, 1.0-500, and 3.0-550μmolL-1, with detection limits of 0.007, 0.6, and 0.9μmolL-1, respectively. The modified electrode was used for the determination of CA, NADH and FA in biological and pharmaceutical samples. © 2013 Elsevier B.V.


Chaharlang B.H.,Islamic Azad University | Bakhtiari A.R.,Tarbiat Modares University | Yavari V.,Khorramshahr Marine Science and Technology University
Bulletin of Environmental Contamination and Toxicology | Year: 2012

The levels of Cd, Cu, Pb and Zn in surface sediment, soft tissue and shell of the oyster Saccostrea cucullata collected from three locations, in the intertidal zones of Lengeh Port, northern part of Persian Gulf were measured. Results indicated that there were a positive correlation across Zn (r = 0.58, p = 0.025), Cd (r = 0.74, p < 0.01) and Cu (r = 0.80, p < 0.01) levels in the soft tissue of oyster and sediment which supported this fact that the soft tissue of S. cucullata can be considered as biomonitoring agent for Cd, Zn and Cu in the Lengeh Port. © Springer Science+Business Media, LLC 2012.


Keyvanshokooh S.,Khorramshahr Marine Science and Technology University | Tahmasebi-Kohyani A.,Khorramshahr Marine Science and Technology University
Aquaculture | Year: 2012

A feeding study was conducted to determine the effect of dietary nucleotides (NT) on growth performance and muscle proteome profile of rainbow trout fingerlings. Five experimental diets according to different levels of supplemented nucleotides (0, 0.05, 0.1, 0.15 and 0.2%) were assayed in rainbow trout for 8 weeks. Each diet was randomly allocated to triplicate groups of fish with initial average weight of approximately 23. g. The percentage of body weight gain (WG) and feed efficiency (FE) of fish were better when the fish were fed 0.15-0.2% diets. At the end of feeding trial, fish fed the basal and 0.2% diets were subjected to proteomic analysis. Proteins of the muscle tissue were analyzed using two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry. Dietary NT caused differential expression of muscle metabolic proteins including glyceraldehyde-3-phosphate dehydrogenase, creatine kinase, adenylate kinase, nucleoside diphosphate kinase, and triosephosphate isomerase. In addition to metabolic enzymes, troponin-T-1 as a structural protein was found to increase in abundance in the treated fish. The altered expression of both metabolic and structural proteins in fish fed NT may be related to higher growth rate in rainbow trout. These findings provide basic information to understand possible mechanisms of dietary NT contribution to better growth in rainbow trout. © 2011 Elsevier B.V.


Ghanemi K.,Khorramshahr Marine Science and Technology University | Nikpour Y.,Khorramshahr Marine Science and Technology University | Omidvar O.,Khorramshahr Marine Science and Technology University | Maryamabadi A.,Khorramshahr Marine Science and Technology University
Talanta | Year: 2011

The application of sulfur-nanoparticle-loaded alumina as an efficient adsorbent for the solid-phase extraction (SPE) and determination of trace amounts of Cd, Cu, Zn, and Pb ions was investigated in marine samples using flame atomic absorption spectrometry (FAAS). The nanometer-sized sulfur particles were synthesized in situ, physically loaded onto alumina microparticles, and the parameters influencing the preconcentration of the analytes, such as the pH, solution flow rate and volume, eluent solution, and interfering ions, were examined. The results showed that the optimal conditions for quantitative recovery of the metal ions by adsorption and elution on the sulfur nanoparticles (SNPs) was achieved by employing a flow rate of 15 mL min-1, a pH of 8.5 for the sample solutions, and an eluent composed of 3.0 mol L-1 HNO3 in methanol. The detection limits of this method for Cd, Zn, Cu, and Pb ions were 0.30, 0.21, 0.24, and 0.63 μg L-1 (n = 10), respectively. Application of the proposed method to the analysis of fish certified reference material (DORM-3) produced results that were in good agreement with the certified values. The proposed method was also successfully applied to the determination of analytes in marine samples, including seawater, fish, and oysters. © 2011 Elsevier B.V. All rights reserved.


Habibi E.,Khorramshahr Marine Science and Technology University | Ghanemi K.,Khorramshahr Marine Science and Technology University | Fallah-Mehrjardi M.,Khorramshahr Marine Science and Technology University | Dadolahi-Sohrab A.,Khorramshahr Marine Science and Technology University
Analytica Chimica Acta | Year: 2013

A novel and efficient digestion method based on choline chloride-oxalic acid (ChCl-Ox) deep eutectic solvent (DES) was developed for flame atomic absorption spectrometry (FAAS) determination of Cu, Zn, and Fe in biological fish samples. Key parameters that influence analyte recovery were investigated and optimized, using the fish protein certified reference material (CRM, DORM-3) throughout the procedure. In this method, 100mg of the sample was dissolved in ChCl-Ox (1:2, molar ratio) at 100°C for 45min. Then, 5.0mL HNO3 (1.0M) was added. After centrifugation, the supernatant solution was filtered, diluted to a known volume, and analyzed by FAAS. Under optimized conditions, an excellent agreement between the obtained results and the certified values was observed, using Student's t-test (P=0.05); the extraction recovery of the all elements was greater than 95.3%. The proposed method was successfully applied to the determination of analytes in different tissues (muscle, liver, and gills) having a broad concentration range in a marine fish sample. The reproducibility of the method was validated by analyzing all samples by our method in a different laboratory, using inductively coupled plasma optical emission spectrometry (ICP-OES). For comparison, a conventional acid digestion (CAD) method was also used for the determination of analytes in all studied samples. The simplicity of the proposed experimental procedure, high extraction efficiency, short analysis time, lack of concentrated acids and oxidizing agents, and the use of safe and inexpensive components demonstrate the high potential of ChCl-Ox (1:2) for routine trace metal analysis in biological samples. © 2012 Elsevier B.V.

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