Kharkiv National Medical University

www.ksmu.kharkov.ua/index.php?lang=en
Kharkiv, Ukraine

Kharkov National Medical University , formerly known as Kharkov Medical Institute and Kharkov State Medical University, is a medical university in Kharkiv, Ukraine. It was first known as Kharkov state medical university.Post addresse: Prospekt Lenina, 4, Kharkiv, 61022, Ukraine.At present, over 700 teachers work at the departments of the university. Staff capacity is 5 corresponding members NAMN Ukraine, 17 Honoured Scientist of Ukraine, 2 Honored high school Ukraine, 13 distinguished doctors of Ukraine, 8 winners of the State Prize of Ukraine in Science and Engineering, 28 academicians of the public academies of Ukraine, 28 employees - Member of International Medical Associations;. Since 1951, the University has been training medical personnel for countries of the Eastern Europe, China and Mongolia, and since 1961 it has been training students from other countries of Asia, Africa and Latin America. At present, there are about 2000 foreign students in the Kharkiv National Medical University who study at the Preparatory Department, Medical, Nursing and Dental Faculties, undergo postgraduate and clinical post-graduate courses as well as professional probation at departments of the University in Dental, therapy, orthopedics, surgery, oncology, urology, psychiatry, ophthalmology, obstetrics and gynecology, as well as other medical specialties. The University has trained over 5000 specialists for 86 states of Europe, Asia, Latin America, Middle East countries. Among them there are 3 Doctors and 70 Candidates of Medical Science, about 200 clinical post-graduates . Wikipedia.

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Grant
Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: ENV.2008.1.2.1.2. | Award Amount: 3.18M | Year: 2009

The research project investigates the possible impact of global climate change on reproductive health in one Arctic and two European populations. The key questions to be addressed are, firstly, how may climate change influence human exposure to widespread environmental contaminants and, secondly, how may contaminants impact occurrence of reproductive disorders as sensitive indicators of health? To provide affirmative answers to these questions the proposal will as a first step identify and describe mechanisms by which a changing climate may affect the exposure of Arctic and other human populations to contaminants through change in chemical use and emissions, delivery to the arctic ecosystem as well as processing within the arctic physical environment and human food chain. This work relies on modelling of existing data. Secondly, the project will expand the existing knowledge database on human exposure to polybrominated biphenylethers, perfluorinated surfactants and phthalates by analyses of 1000 biobanked serum samples collected in a EU FP5 project. Thirdly, the project will increase the limited knowledge on links between human exposure to contaminants and reproductive health. This work relies on a large existing parent-child-cohort, where a follow-up survey provide new data that are fed into risk assessment. Furthermore we will perform reviews of experimental and epidemiological literature to identify critical reproductive effects and exposure-response data for selected compounds as input to the risk assessment. Finally the project will integrate data on climate induced changes in contaminant mobility and distribution and links between contaminant exposure and reproductive health into a risk evaluation providing insight into possible future risk scenarios related to global climate change. The project draws upon a network of experts in climate modelling and in experimental, epidemiological and risk assessment methodologies and builds upon three established cohorts in Greenland, Poland and Ukraine.


Boiagina O.,Kharkiv National Medical University
Georgian medical news | Year: 2017

The structure of the corpus callosum is a certain form of order of the nerve fibers, glial cells and blood microvessels and it is actually unexplored. We set the goal to understand the general constructive principle of the myeloarchitectonics of human corpus callosum. We used whole mounts of the corpus callosum (5 men and 5 women aged from 36 to 60 years) after their two-week fixation in 10% formalin solution. The next stage was to dissect plate sections of the corpus callosum brainstem in two mutually perpendicular planes. Some of them were subjected to impregnation in 1% osmium tetroxide solution, according to the method adopted in transmission electron microscopy. To prepare these plate sections of the corpus callosum for further study in the light microscope we used the method of plastination in epoxy resin. After complete polymerization plastinated mounts were used for making slices. For further research at high magnification light microscopy they were thinned up to 0.3 mm thickness and were subjected to coloration using 1% solution of methylene blue on 1% borax solution. They were studied using a binocular microscope МБС-9 and microscope "Konus" equipped with digital camera. It was found that the human corpus callosum consists of a number of transversely oriented bands of nerve fibers (commissural cords). Each of them consists of a tightly appressed stratified sections, fascicular rations, which are separated by interstitial layers. In turn, these interfascicular layers give short lateral spurs that divide fascicular rations into individual segments - subfascicular rations. Multiple cells containing interfascicular oligodendrocytes associated with individual subfascicular sets of nerve tracts are dispersed in the cluster order among myelinated nerve fibers of fascicular rations. Fundamentally important point is that the interstitial layers in the corpus callosum as a whole form a complex three-dimensional network structure which is subordinated to the nature of branching of blood microcirculation vessels of capillary type, being simultaneously the reticular system, performing extravascular circulation of fluid containing dissolved nutrients.


Garmash O.,Kharkiv National Medical University
Georgian medical news | Year: 2017

The paper aims at studying the effect of body overweight at birth on the dental health of 482 children in the Kharkiv City (Ukraine) during their first year of life over the 2001 and 2013 interval. The macrosomia set is comprised of the medical records of the children born with fetal macrosomia, and the normosimia set of the medical records of the children born with weight and height that correspond to the gestation age. The gestation age of all children is 37 to 42 weeks'. To determine the average time of first tooth eruption and deciduous teeth growth rate for each of the sets under study, we have used the hypothesis about a linear dependence between the number of erupted teeth and the age of the child. Processing statistical data is performed applying the multiple linear regression analysis. The reasons for macrosomia in the children are examined. The number of pregnancies and deliveries influence the likelihood of having a child with fetal macrosomia. A greater likelihood of having a child with fetal macrosomia in the older parents is not found. The correlation between the states of a child at birth (macrosomia/normosimia) and terms of deciduous tooth eruption (the delayed/timely/early eruption) expressed in a number of teeth at the age of one year is determined. The difference in the teeth growth rate between the boys and girls within the both sets are insignificant. The children born with macrosomia have a lower rate (approximately 0.1 tooth per month) of teeth growth and a greater spread in the number of teeth that erupt by a certain age.


Stepanenko A.Y.,Kharkiv National Medical University
Neuroscience and Behavioral Physiology | Year: 2015

The aim of the present work was to study the relationship between cerebellum weight and its developmental dynamics on the one hand and body length and type of physique on the other. Studies involved 295 corpses of both genders (173 men and 122 women) dying at age 20–99 years. Body length was measured, along with the cross-sectional diameter of the thoracic cage and cerebellum weight. Somatotypes were identified using the Rice-Eysenck index. Human cerebellum weight from was found to vary over the range 103–197 g (mean 144 ± 1.0 g) and was significantly greater in men than women (150.5 ± 1.3 g and 133.9 ± 1.2 g, p 0.001). Age had a greater influence on cerebellar weight in men than in women (R = –0.46 and –0.43, respectively). In men, the period of relatively stable cerebellar weight lasted to about 50 years of age, which was followed by a period of decreasing cerebellar weight. In women, the stable period lasted to about 70 years. Cerebellar weight was related to body length (R = 0.35 for men and R = 0.36 for women). The relationship between cerebellum weight and body length in men (1.0 g/cm) was greater than that in women (0.5 g/cm): the differences in cerebellum weights in men and women increased with increases in body length. Differences in cerebellum weight in people with different types of physique were minor © 2015 Springer Science+Business Media New York.


Gorbenko G.P.,Kharkiv National Medical University
Journal of Fluorescence | Year: 2011

Fluorescence spectroscopy is one of the most powerful tools for characterization of a multitude of biological processes. Of these, the phenomenon of protein oligomerization attracts especial interest due to its crucial role in the formation of fibrillar protein aggregates (amyloid fibrils) involved in ethiology of so-called protein misfolding diseases. It is becoming increasingly substantiated that protein fibrillization in vivo can be initiated and modulated at membrane-water interface. All steps of membrane-assisted fibrillogenesis, viz., protein adsorption onto lipid bilayer, structural transition of polypeptide chain into a highly aggregation-prone partially folded conformation, assembly of oligomeric nucleus from membrane-bound monomeric species and fiber elongation can be monitored with a mighty family of fluorescence-based techniques. Furthermore, the mechanisms behind cytotoxicity of prefibrillar protein oligomers are highly amenable to fluorescence analysis. The applications of fluorescence spectroscopy to monitoring protein oligomerization in a membrane environment are exemplified and some problems encountered in such kinds of studies are highlighted. © Springer Science+Business Media, LLC 2011.


Trusova V.,Kharkiv National Medical University
Cellular and Molecular Biology Letters | Year: 2012

The molecular details of interactions between lipid membranes and lysozyme (Lz), a small polycationic protein with a wide range of biological activities, have long been the focus of numerous studies. The biological consequences of this process are considered to embrace at least two aspects: i) correlation between antimicrobial and membranotropic properties of this protein, and ii) lipid-mediated Lz amyloidogenesis. The mechanisms underlying the lipid-assisted protein fibrillogenesis and membrane disruption exerted by Lz in bacterial cells are believed to be similar. The present investigation was undertaken to gain further insight into Lz-lipid interactions and explore the routes by which Lz exerts its antimicrobial and amyloidogenic actions. Binding and Förster resonance energy transfer studies revealed that upon increasing the content of anionic lipids in lipid vesicles, Lz forms aggregates in a membrane environment. Total internal reflection fluorescence microscopy and pyrene excimerization reaction were employed to study the effect of Lz on the structural and dynamic properties of lipid bilayers. It was found that Lz induces lipid demixing and reduction of bilayer free volume, the magnitude of this effect being much more pronounced for oligomeric protein. © 2012 Versita Warsaw and Springer-Verlag Wien.


Kon K.V.,Kharkiv National Medical University | Rai M.K.,Sant Gadge Baba Amravati University
Expert Review of Anti-Infective Therapy | Year: 2012

Antibiotic resistance is documented to be a serious problem that affects the choice of appropriate antibiotic therapy and increases the probability of unfavorable infection outcome. One of the proposed methods to cope with multidrug-resistant (MDR) bacteria is the use of alternative antibacterial treatments, which include natural antimicrobial substances such as plant essential oils (EOs). The aim of the present article is to review published studies on the activity of EOs and their constituents against MDR bacteria and to formulate perspectives for the future. In general, published studies indicate that EOs can be used as effective antiseptics against many species, including MDR bacteria, such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, resistant isolates of Pseudomonas aeruginosa, Klebsiella pneumoniae and others; certain EOs may potentiate the effectiveness of antibiotics against MDR bacteria; EOs can be synergistic with bacteriophages; and polymeric nanoparticles can be used for delivery of EOs and enhancement of their activity at the site of infection. © 2012 2012 Expert Reviews Ltd.


Trusova V.M.,Kharkiv National Medical University
Biophysical Reviews and Letters | Year: 2015

Amyloid fibrils represent a generic class of mechanically strong and stable biomaterials with extremely advantageous properties. Although amyloids were initially associated only with severe neurological disorders, the role of these structures nowadays is shifting from health debilitating to highly beneficial both in biomedical and technological aspects. Intensive involvement of fibrillar assemblies into the wide range of pathogenic and functional processes strongly necessitate the molecular level characterization of the structural, physical and elastic features of protein nanofibrils. In the present contribution, we made an attempt to highlight the up-to-date progress in the understanding of amyloid properties from the polymer physics standpoint. The fundamental insights into protein fibril behavior are essential not only for development of therapeutic strategies to combat the protein misfolding disorders but also for rational and precise design of novel biodegradable protein-based nanopolymers. © 2015 World Scientific Publishing Company.


Gorbenko G.,Kharkiv National Medical University | Trusova V.,Kharkiv National Medical University
Advances in Protein Chemistry and Structural Biology | Year: 2011

Biological membranes are featured by a remarkable ability to modulate a wide range of physiological and pathological processes. Of these, protein aggregation is currently receiving the greatest attention, as one type of the ordered protein aggregates, amyloid fibrils, proved to be involved in molecular etiology of a number of fatal diseases. It has been hypothesized that nucleation of amyloid fibrils and toxic action of their precursors is mediated by lipid-protein interactions. Lipid bilayer provides a variety of environments in which aggregated state of polypeptide chain appears to be more thermodynamically favorable than its monomeric form. The major factors responsible for the enhanced self-association propensity of membrane-bound proteins include (i) structural transition of polypeptide chain into aggregation-prone conformation; (ii) protein crowding in a lipid phase; (iii) particular aggregation-favoring orientation and bilayer embedment of the protein molecules. All these factors are considered in the present review with an emphasis being put on the role of electrostatic, hydrophobic, and hydrogen-bonding phenomena in initiating and modulating the protein aggregation on a membrane template. Likewise, we survey the advanced experimental techniques employed for detection and structural characterization of the aggregated species in membrane systems. © 2011 Elsevier Inc. All rights reserved.


Gorbenko G.,Kharkiv National Medical University | Trusova V.,Kharkiv National Medical University
Biophysical Chemistry | Year: 2011

The ability of oligomeric lysozyme to modify the molecular organization of the model bilayer membranes composed of phosphatidylcholine (PC) and its mixtures with phosphatidylglycerol (PG) or cholesterol (Chol) was assessed using fluorescent probes 6-propionyl-2-dimethylaminonaphthalene (Prodan), 4-dimethylaminochalcone (DMC), pyrene and 1,6-diphenyl-1,3,5-hexatriene (DPH). The observed changes in the fluorescence characteristics of polarity-sensitive probes Prodan and DMC, located in interfacial bilayer region, were interpreted due to the partial dehydration of the glycerol backbone, which was under the influence of aggregated protein. Cholesterol was found to prevent the perturbations of membrane polar part by lysozyme aggregates. Analysis of the pyrene excimerization data revealed an oligomer-induced reduction in bilayer free volume, presumably caused by an increased packing density of hydrocarbon chains. This effect proved to be virtually independent of membrane composition. It was demonstrated that membranotropic activity of oligomeric lysozyme markedly exceeds that of monomeric protein. The biological significance of the results obtained is twofold, implicating the general membrane-mediated mechanisms of oligomer toxicity and specific pathways of lysozyme fibrillogenesis in vivo associated with familial nonneuropathic systemic amyloidosis. © 2011 Elsevier B.V.

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