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Kharkiv, Ukraine

Kharkov National Medical University , formerly known as Kharkov Medical Institute and Kharkov State Medical University, is a medical university in Kharkiv, Ukraine. It was first known as Kharkov state medical university.Post addresse: Prospekt Lenina, 4, Kharkiv, 61022, Ukraine.At present, over 700 teachers work at the departments of the university. Staff capacity is 5 corresponding members NAMN Ukraine, 17 Honoured Scientist of Ukraine, 2 Honored high school Ukraine, 13 distinguished doctors of Ukraine, 8 winners of the State Prize of Ukraine in Science and Engineering, 28 academicians of the public academies of Ukraine, 28 employees - Member of International Medical Associations;. Since 1951, the University has been training medical personnel for countries of the Eastern Europe, China and Mongolia, and since 1961 it has been training students from other countries of Asia, Africa and Latin America. At present, there are about 2000 foreign students in the Kharkiv National Medical University who study at the Preparatory Department, Medical, Nursing and Dental Faculties, undergo postgraduate and clinical post-graduate courses as well as professional probation at departments of the University in Dental, therapy, orthopedics, surgery, oncology, urology, psychiatry, ophthalmology, obstetrics and gynecology, as well as other medical specialties. The University has trained over 5000 specialists for 86 states of Europe, Asia, Latin America, Middle East countries. Among them there are 3 Doctors and 70 Candidates of Medical Science, about 200 clinical post-graduates . Wikipedia.

Gorbenko G.P.,Kharkiv National Medical University
Journal of Fluorescence | Year: 2011

Fluorescence spectroscopy is one of the most powerful tools for characterization of a multitude of biological processes. Of these, the phenomenon of protein oligomerization attracts especial interest due to its crucial role in the formation of fibrillar protein aggregates (amyloid fibrils) involved in ethiology of so-called protein misfolding diseases. It is becoming increasingly substantiated that protein fibrillization in vivo can be initiated and modulated at membrane-water interface. All steps of membrane-assisted fibrillogenesis, viz., protein adsorption onto lipid bilayer, structural transition of polypeptide chain into a highly aggregation-prone partially folded conformation, assembly of oligomeric nucleus from membrane-bound monomeric species and fiber elongation can be monitored with a mighty family of fluorescence-based techniques. Furthermore, the mechanisms behind cytotoxicity of prefibrillar protein oligomers are highly amenable to fluorescence analysis. The applications of fluorescence spectroscopy to monitoring protein oligomerization in a membrane environment are exemplified and some problems encountered in such kinds of studies are highlighted. © Springer Science+Business Media, LLC 2011.

Chaychenko T.,Kharkiv National Medical University
Polish Annals of Medicine | Year: 2016

Introduction: Pediatric obesity reflects a real crisis for public health as associated with cardiovascular risk in subjects with developed metabolic syndrome. Simultaneously the information concerning risk related cardiovascular changes in metabolically healthy obese adolescents is pretty insufficient. Aim: This study is designed to determine the risk related cardiovascular changes in metabolically healthy obese adolescents. Material and methods: 208 obese adolescents were grouped as metabolically healthy and metabolically unhealthy by International Diabetes Federation (IDF) criteria for pediatric metabolic syndrome. We analyzed the basic metabolic parameters, left ventricular geometry and function, 24-hours blood pressure monitoring and carotid intima-media thickness. Control group consisted of 23 lean healthy subjects. Results and discussion: 69% of obese adolescents could be considered as metabolically healthy by pediatric IDF criteria. BMI in metabolically unhealthy was greater vs. metabolically healthy (P = 0.019) as well as dyslipidemia and dysglicemia. Cardiovascular parameters were deteriorated in all obese vs. lean healthy (myocardial hypertrophy and dysfunction, thickening of carotid vessels and systolic hypertension). It established low sensitivity (0.28) and low negative predictive value (0.29) of metabolic syndrome criteria to screen obesity associated cardiovascular problems. Conclusions: Prognostic capability of pediatric metabolic syndrome criteria is pretty low due to its sensitivity. Therefore obese adolescents not met diagnostic level for metabolic syndrome by IDF criteria could be falsely excluded from the cardiovascular risk group. Thus, it is not possible to assert an existence of absolutely healthy metabolic profile in obese and more sensitive markers are necessary for the metabolically healthy obesity identification. © 2016 Warmińsko-Mazurska Izba Lekarska w Olsztynie.

Trusova V.M.,Kharkiv National Medical University
Biophysical Reviews and Letters | Year: 2015

Amyloid fibrils represent a generic class of mechanically strong and stable biomaterials with extremely advantageous properties. Although amyloids were initially associated only with severe neurological disorders, the role of these structures nowadays is shifting from health debilitating to highly beneficial both in biomedical and technological aspects. Intensive involvement of fibrillar assemblies into the wide range of pathogenic and functional processes strongly necessitate the molecular level characterization of the structural, physical and elastic features of protein nanofibrils. In the present contribution, we made an attempt to highlight the up-to-date progress in the understanding of amyloid properties from the polymer physics standpoint. The fundamental insights into protein fibril behavior are essential not only for development of therapeutic strategies to combat the protein misfolding disorders but also for rational and precise design of novel biodegradable protein-based nanopolymers. © 2015 World Scientific Publishing Company.

Kon K.V.,Kharkiv National Medical University | Rai M.K.,Sant Gadge Baba Amravati University
Expert Review of Anti-Infective Therapy | Year: 2012

Antibiotic resistance is documented to be a serious problem that affects the choice of appropriate antibiotic therapy and increases the probability of unfavorable infection outcome. One of the proposed methods to cope with multidrug-resistant (MDR) bacteria is the use of alternative antibacterial treatments, which include natural antimicrobial substances such as plant essential oils (EOs). The aim of the present article is to review published studies on the activity of EOs and their constituents against MDR bacteria and to formulate perspectives for the future. In general, published studies indicate that EOs can be used as effective antiseptics against many species, including MDR bacteria, such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, resistant isolates of Pseudomonas aeruginosa, Klebsiella pneumoniae and others; certain EOs may potentiate the effectiveness of antibiotics against MDR bacteria; EOs can be synergistic with bacteriophages; and polymeric nanoparticles can be used for delivery of EOs and enhancement of their activity at the site of infection. © 2012 2012 Expert Reviews Ltd.

Trusova V.,Kharkiv National Medical University
Cellular and Molecular Biology Letters | Year: 2012

The molecular details of interactions between lipid membranes and lysozyme (Lz), a small polycationic protein with a wide range of biological activities, have long been the focus of numerous studies. The biological consequences of this process are considered to embrace at least two aspects: i) correlation between antimicrobial and membranotropic properties of this protein, and ii) lipid-mediated Lz amyloidogenesis. The mechanisms underlying the lipid-assisted protein fibrillogenesis and membrane disruption exerted by Lz in bacterial cells are believed to be similar. The present investigation was undertaken to gain further insight into Lz-lipid interactions and explore the routes by which Lz exerts its antimicrobial and amyloidogenic actions. Binding and Förster resonance energy transfer studies revealed that upon increasing the content of anionic lipids in lipid vesicles, Lz forms aggregates in a membrane environment. Total internal reflection fluorescence microscopy and pyrene excimerization reaction were employed to study the effect of Lz on the structural and dynamic properties of lipid bilayers. It was found that Lz induces lipid demixing and reduction of bilayer free volume, the magnitude of this effect being much more pronounced for oligomeric protein. © 2012 Versita Warsaw and Springer-Verlag Wien.

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