Kharkiv, Ukraine

Kharkov National Medical University , formerly known as Kharkov Medical Institute and Kharkov State Medical University, is a medical university in Kharkiv, Ukraine. It was first known as Kharkov state medical university.Post addresse: Prospekt Lenina, 4, Kharkiv, 61022, Ukraine.At present, over 700 teachers work at the departments of the university. Staff capacity is 5 corresponding members NAMN Ukraine, 17 Honoured Scientist of Ukraine, 2 Honored high school Ukraine, 13 distinguished doctors of Ukraine, 8 winners of the State Prize of Ukraine in Science and Engineering, 28 academicians of the public academies of Ukraine, 28 employees - Member of International Medical Associations;. Since 1951, the University has been training medical personnel for countries of the Eastern Europe, China and Mongolia, and since 1961 it has been training students from other countries of Asia, Africa and Latin America. At present, there are about 2000 foreign students in the Kharkiv National Medical University who study at the Preparatory Department, Medical, Nursing and Dental Faculties, undergo postgraduate and clinical post-graduate courses as well as professional probation at departments of the University in Dental, therapy, orthopedics, surgery, oncology, urology, psychiatry, ophthalmology, obstetrics and gynecology, as well as other medical specialties. The University has trained over 5000 specialists for 86 states of Europe, Asia, Latin America, Middle East countries. Among them there are 3 Doctors and 70 Candidates of Medical Science, about 200 clinical post-graduates . Wikipedia.

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Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: ENV.2008. | Award Amount: 3.18M | Year: 2009

The research project investigates the possible impact of global climate change on reproductive health in one Arctic and two European populations. The key questions to be addressed are, firstly, how may climate change influence human exposure to widespread environmental contaminants and, secondly, how may contaminants impact occurrence of reproductive disorders as sensitive indicators of health? To provide affirmative answers to these questions the proposal will as a first step identify and describe mechanisms by which a changing climate may affect the exposure of Arctic and other human populations to contaminants through change in chemical use and emissions, delivery to the arctic ecosystem as well as processing within the arctic physical environment and human food chain. This work relies on modelling of existing data. Secondly, the project will expand the existing knowledge database on human exposure to polybrominated biphenylethers, perfluorinated surfactants and phthalates by analyses of 1000 biobanked serum samples collected in a EU FP5 project. Thirdly, the project will increase the limited knowledge on links between human exposure to contaminants and reproductive health. This work relies on a large existing parent-child-cohort, where a follow-up survey provide new data that are fed into risk assessment. Furthermore we will perform reviews of experimental and epidemiological literature to identify critical reproductive effects and exposure-response data for selected compounds as input to the risk assessment. Finally the project will integrate data on climate induced changes in contaminant mobility and distribution and links between contaminant exposure and reproductive health into a risk evaluation providing insight into possible future risk scenarios related to global climate change. The project draws upon a network of experts in climate modelling and in experimental, epidemiological and risk assessment methodologies and builds upon three established cohorts in Greenland, Poland and Ukraine.

Trusova V.M.,Kharkiv National Medical University
YSF 2015 - International Young Scientists Forum on Applied Physics | Year: 2015

A wide range of peptides and proteins has the unique propensity to self-assemble into insoluble fibrillar quaternary structure enriched in intermolecularly hydrogen bonded β-sheets [1,2]. The accumulation of these highly ordered aggregates in human tissues is associated with a number of devastating disorders, such as Alzheimer's, Parkinson's, and Huntington's diseases, type II diabetes, atrial amyloidosis, etc. [3-5]. It is now generally accepted that molecular basis of amyloid formation lies in the incorrect protein folding, or misfolding [6,7]. This is readily achievable because the compactness of native state is compromised by the loss of configurational entropy during polypeptide folding and repulsive electrostatic interactions. Due to small difference between stabilizing and destabilizing forces, native protein structure is only marginally stable, so that any variation in physicochemical properties of polypeptide surroundings may trigger the protein leaving of the native folding pathway and entering the off-folding route, one of which is formation of the stable fibrillary aggregates from the unstable partially unfolded intermediates. © 2015 IEEE.

Molodan D.V.,Kharkiv National Medical University
New Armenian Medical Journal | Year: 2016

The occurrence of such pathological conditions as hypertension and obesity are closely related to the metabolic, neurohormonal and hemodynamic disturbances affecting the state of endothelium. The influence of hyperuricemia on endothelial function in these patients is understudied, particularly in combination with other metabolic changes. The aim of the study was to investigate endothelial function and metabolic abnormalities in patients with hypertension associated with obesity in the pr esence of hyperuricemia. Totally 108 patients (52 (40%) males, 78 (60%) females) with hypertension of I and II stage, 1-3 degree of blood pressure increase and with obesity of I-II degree were examined during the study. The mean age of the examined patients was 58.0±0.6 years. All patients were divided into two groups depending on the level of hyperuricemia: patients with normouricemia (n=46) and patients with asymptomatic hyperuricemia (n=62). Control group was consisted of 12 practically healthy normotensive patients matched by sex and age with patie nts of the main group. Endothelium-dependent vasodilatation was significantly reduced in patients with normouricemia and asymptomatic hyperuricemia in comparison with control group, the level of nitric oxide metabolites (NO2+NO3) was increased in the main group compared to the control group. The study of microalbuminuria and asymmetric dimethylarginine indicated the increase of these indices in hypertensive patients with obesity; particularly they were the highest in patients with asymptomatic hyperuricemia. Development of combined dyslipidemia and hyperinsulinism was identified in hypertensive patients with obesity, more pronounced in patients with high levels of uric acid in blood. Deterioration of purine metabolism, lipids and carbohydrates causes an adverse effect on the state of endothelium. Taking into account the correlation between endothelium-dependent vasodilatation and uric acid it can be assumed, that uric acid has a damaging effect on endothelium. Endothelial dysfunction was noted in patients with essential hypertension associated with obesity, resulting in the decrease of endothelium-dependent vasodilatation, increase of the level of nitric oxide metabolites, asymmetric dimethylarginine and microalbuminuria. The deterioration of endothelial function was more expressed in patients with asymptomatic hyperuricemia. In multiple regression analysis it was revealed that changes of endothelium-dependent vasodilatation largely depend on such factors as uric acid, low-density lipoproteins, high-density lipoproteins, glucose, body mass index. © 2016 Yerevan State Medical University after Mkhitar Heratsi. All Rights Reserved.

Gorbenko G.P.,Kharkiv National Medical University
Journal of Fluorescence | Year: 2011

Fluorescence spectroscopy is one of the most powerful tools for characterization of a multitude of biological processes. Of these, the phenomenon of protein oligomerization attracts especial interest due to its crucial role in the formation of fibrillar protein aggregates (amyloid fibrils) involved in ethiology of so-called protein misfolding diseases. It is becoming increasingly substantiated that protein fibrillization in vivo can be initiated and modulated at membrane-water interface. All steps of membrane-assisted fibrillogenesis, viz., protein adsorption onto lipid bilayer, structural transition of polypeptide chain into a highly aggregation-prone partially folded conformation, assembly of oligomeric nucleus from membrane-bound monomeric species and fiber elongation can be monitored with a mighty family of fluorescence-based techniques. Furthermore, the mechanisms behind cytotoxicity of prefibrillar protein oligomers are highly amenable to fluorescence analysis. The applications of fluorescence spectroscopy to monitoring protein oligomerization in a membrane environment are exemplified and some problems encountered in such kinds of studies are highlighted. © Springer Science+Business Media, LLC 2011.

Chaychenko T.,Kharkiv National Medical University
Polish Annals of Medicine | Year: 2016

Introduction: Pediatric obesity reflects a real crisis for public health as associated with cardiovascular risk in subjects with developed metabolic syndrome. Simultaneously the information concerning risk related cardiovascular changes in metabolically healthy obese adolescents is pretty insufficient. Aim: This study is designed to determine the risk related cardiovascular changes in metabolically healthy obese adolescents. Material and methods: 208 obese adolescents were grouped as metabolically healthy and metabolically unhealthy by International Diabetes Federation (IDF) criteria for pediatric metabolic syndrome. We analyzed the basic metabolic parameters, left ventricular geometry and function, 24-hours blood pressure monitoring and carotid intima-media thickness. Control group consisted of 23 lean healthy subjects. Results and discussion: 69% of obese adolescents could be considered as metabolically healthy by pediatric IDF criteria. BMI in metabolically unhealthy was greater vs. metabolically healthy (P = 0.019) as well as dyslipidemia and dysglicemia. Cardiovascular parameters were deteriorated in all obese vs. lean healthy (myocardial hypertrophy and dysfunction, thickening of carotid vessels and systolic hypertension). It established low sensitivity (0.28) and low negative predictive value (0.29) of metabolic syndrome criteria to screen obesity associated cardiovascular problems. Conclusions: Prognostic capability of pediatric metabolic syndrome criteria is pretty low due to its sensitivity. Therefore obese adolescents not met diagnostic level for metabolic syndrome by IDF criteria could be falsely excluded from the cardiovascular risk group. Thus, it is not possible to assert an existence of absolutely healthy metabolic profile in obese and more sensitive markers are necessary for the metabolically healthy obesity identification. © 2016 Warmińsko-Mazurska Izba Lekarska w Olsztynie.

Trusova V.,Kharkiv National Medical University
Cellular and Molecular Biology Letters | Year: 2012

The molecular details of interactions between lipid membranes and lysozyme (Lz), a small polycationic protein with a wide range of biological activities, have long been the focus of numerous studies. The biological consequences of this process are considered to embrace at least two aspects: i) correlation between antimicrobial and membranotropic properties of this protein, and ii) lipid-mediated Lz amyloidogenesis. The mechanisms underlying the lipid-assisted protein fibrillogenesis and membrane disruption exerted by Lz in bacterial cells are believed to be similar. The present investigation was undertaken to gain further insight into Lz-lipid interactions and explore the routes by which Lz exerts its antimicrobial and amyloidogenic actions. Binding and Förster resonance energy transfer studies revealed that upon increasing the content of anionic lipids in lipid vesicles, Lz forms aggregates in a membrane environment. Total internal reflection fluorescence microscopy and pyrene excimerization reaction were employed to study the effect of Lz on the structural and dynamic properties of lipid bilayers. It was found that Lz induces lipid demixing and reduction of bilayer free volume, the magnitude of this effect being much more pronounced for oligomeric protein. © 2012 Versita Warsaw and Springer-Verlag Wien.

Kon K.V.,Kharkiv National Medical University | Rai M.K.,Sant Gadge Baba Amravati University
Expert Review of Anti-Infective Therapy | Year: 2012

Antibiotic resistance is documented to be a serious problem that affects the choice of appropriate antibiotic therapy and increases the probability of unfavorable infection outcome. One of the proposed methods to cope with multidrug-resistant (MDR) bacteria is the use of alternative antibacterial treatments, which include natural antimicrobial substances such as plant essential oils (EOs). The aim of the present article is to review published studies on the activity of EOs and their constituents against MDR bacteria and to formulate perspectives for the future. In general, published studies indicate that EOs can be used as effective antiseptics against many species, including MDR bacteria, such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, resistant isolates of Pseudomonas aeruginosa, Klebsiella pneumoniae and others; certain EOs may potentiate the effectiveness of antibiotics against MDR bacteria; EOs can be synergistic with bacteriophages; and polymeric nanoparticles can be used for delivery of EOs and enhancement of their activity at the site of infection. © 2012 2012 Expert Reviews Ltd.

Trusova V.M.,Kharkiv National Medical University
Biophysical Reviews and Letters | Year: 2015

Amyloid fibrils represent a generic class of mechanically strong and stable biomaterials with extremely advantageous properties. Although amyloids were initially associated only with severe neurological disorders, the role of these structures nowadays is shifting from health debilitating to highly beneficial both in biomedical and technological aspects. Intensive involvement of fibrillar assemblies into the wide range of pathogenic and functional processes strongly necessitate the molecular level characterization of the structural, physical and elastic features of protein nanofibrils. In the present contribution, we made an attempt to highlight the up-to-date progress in the understanding of amyloid properties from the polymer physics standpoint. The fundamental insights into protein fibril behavior are essential not only for development of therapeutic strategies to combat the protein misfolding disorders but also for rational and precise design of novel biodegradable protein-based nanopolymers. © 2015 World Scientific Publishing Company.

Gorbenko G.,Kharkiv National Medical University | Trusova V.,Kharkiv National Medical University
Advances in Protein Chemistry and Structural Biology | Year: 2011

Biological membranes are featured by a remarkable ability to modulate a wide range of physiological and pathological processes. Of these, protein aggregation is currently receiving the greatest attention, as one type of the ordered protein aggregates, amyloid fibrils, proved to be involved in molecular etiology of a number of fatal diseases. It has been hypothesized that nucleation of amyloid fibrils and toxic action of their precursors is mediated by lipid-protein interactions. Lipid bilayer provides a variety of environments in which aggregated state of polypeptide chain appears to be more thermodynamically favorable than its monomeric form. The major factors responsible for the enhanced self-association propensity of membrane-bound proteins include (i) structural transition of polypeptide chain into aggregation-prone conformation; (ii) protein crowding in a lipid phase; (iii) particular aggregation-favoring orientation and bilayer embedment of the protein molecules. All these factors are considered in the present review with an emphasis being put on the role of electrostatic, hydrophobic, and hydrogen-bonding phenomena in initiating and modulating the protein aggregation on a membrane template. Likewise, we survey the advanced experimental techniques employed for detection and structural characterization of the aggregated species in membrane systems. © 2011 Elsevier Inc. All rights reserved.

Gorbenko G.,Kharkiv National Medical University | Trusova V.,Kharkiv National Medical University
Biophysical Chemistry | Year: 2011

The ability of oligomeric lysozyme to modify the molecular organization of the model bilayer membranes composed of phosphatidylcholine (PC) and its mixtures with phosphatidylglycerol (PG) or cholesterol (Chol) was assessed using fluorescent probes 6-propionyl-2-dimethylaminonaphthalene (Prodan), 4-dimethylaminochalcone (DMC), pyrene and 1,6-diphenyl-1,3,5-hexatriene (DPH). The observed changes in the fluorescence characteristics of polarity-sensitive probes Prodan and DMC, located in interfacial bilayer region, were interpreted due to the partial dehydration of the glycerol backbone, which was under the influence of aggregated protein. Cholesterol was found to prevent the perturbations of membrane polar part by lysozyme aggregates. Analysis of the pyrene excimerization data revealed an oligomer-induced reduction in bilayer free volume, presumably caused by an increased packing density of hydrocarbon chains. This effect proved to be virtually independent of membrane composition. It was demonstrated that membranotropic activity of oligomeric lysozyme markedly exceeds that of monomeric protein. The biological significance of the results obtained is twofold, implicating the general membrane-mediated mechanisms of oligomer toxicity and specific pathways of lysozyme fibrillogenesis in vivo associated with familial nonneuropathic systemic amyloidosis. © 2011 Elsevier B.V.

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