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Khabarovsk Vtoroy, Russia

Lvov D.K.,Russian Academy of Medical Sciences | Yashkulov K.B.,Board of Federal Service | Prilipov A.G.,Russian Academy of Medical Sciences | Burtseva E.I.,Russian Academy of Medical Sciences | And 23 more authors.
Voprosy Virusologii | Year: 2010

The paper analyzes the amino acid sequence of the receptor-binding site of hemagglutinin (HA) in the variants of pandemic Influenza A/H1N1 swi from 18 patients with moderate (n = 1) and fatal (n = 17) forms of the disease in 2009. Nine samples contained asparaginic acid at position 222 of HA1 (D). This site exhibited mutations in 9 samples: D222G (n = 3), D222N (n = 3), and D222G/D222N (n = 3). In one patient with the moderate form of the disease, D222G mutation was revealed after the second passage in the developing chick embryos; this mutation was not found In the primary sample from the patient. The findings suggest the mutant variants of the virus start to circulate among the population, which requires, firstly, continuation of molecular virological monitoring of the pandemic situation and, secondly, further study of the impact of amino acid substitutions at the receptor-binding site of HA1 on the Increased virulence of influenza A virus.

Abramov S.A.,Russian Academy of Sciences | Dupal T.A.,Russian Academy of Sciences | Zdanovskaya N.I.,Khabarovsk Antiplague Station | Pozdnyakov A.A.,Russian Academy of Sciences | And 2 more authors.
Contemporary Problems of Ecology | Year: 2011

New foci of hantavirus infection were found in Altai and Krasnoyarsk krais, in Novosibirsk, Kemerovo, and Tomsk oblasts, and Altai Republic. It is shown that hantaviruses are distributed in all landscape zones and subzones. The bank vole, the gray red-backed vole, the northern red-backed vole, the narrow-headed vole, the field vole, the root vole, the steppe lemming, and the Korean field and striped mice. © 2011 Pleiades Publishing, Ltd.

Rar V.A.,Russian Academy of Sciences | Epikhina T.I.,Russian Academy of Sciences | Pukhovskaya N.M.,Khabarovsk Antiplague Station | Vysochina N.P.,Khabarovsk Antiplague Station | Ivanov L.I.,Khabarovsk Antiplague Station
Molecular Genetics, Microbiology and Virology | Year: 2013

In total, 484 Haemaphysalis japonica, 359 Haemaphysalis concinna, and 221 Dermacentor silvarum collected in Amur oblast and Khabarovsk krai of the Russian Far East were examined regarding the presence of Anaplasmataceae bacteria using nested PCR. All positive samples were characterized by analysis of the 16S rRNA gene and/or groESL operone nucleotide sequences. Forty-nine H. japonica and three H. concinna were shown to contain DNA of two new Ehrlichia genetic variants. On the basis of 16S rRNA gene and groESL operone nucleotide sequences analysis, these genetic variants were found to be most closely related to Ehrlichia spp. revealed in Haemaphysalis spp. ticks in Japan. Four H. concinna from Amur oblast were shown to contain DNA of a new Anaplasma bovis genetic variant, which corresponded to the A. bovis genetic variant revealed in a red gray-backed vole and a Siberian chipmunk from the Far East. Three H. concinna and nine D. silvarum contained DNA of atypical bacteria that cannot be attributed to any Anaplasmataceae genera based on the determined sequences of the 16S rRNA gene fragments. The revealed atypical bacteria significantly differed from each other and did not form a separate genetic group on the basis of 16S rRNA gene sequences. © 2013 Allerton Press, Inc.

Fomenko N.V.,Russian Academy of Sciences | Livanova N.N.,Russian Academy of Sciences | Borgoyakov V.Y.,Russian Academy of Sciences | Kozlova I.V.,Gamaleya Institute of Epidemiology and Microbiology | And 6 more authors.
Entomological Review | Year: 2010

Unfed adult ticks Ixodes persulcatus from five regions of Russia were examined by PCR method in order to analyze distribution and diversity of B. miyamotoi. B. miyamotoi DNA was found in 1.8, 2.9, 4.5, 2.3, and 2.5% of ticks from Leningrad, Sverdlovsk, Novosibirsk, and Irkutsk provinces, and from Khabarovsk Territory, respectively. Molecular typing of B. miyamotoi DNA was based on the partial sequencing of the 16S rRNA, p66, and glpQ genes. A single genetic variant of B. miyamotoi was detected in all the samples of ticks collected from five regions. © 2010 Pleiades Publishing, Ltd.

Rar V.A.,Russian Academy of Sciences | Epikhina T.I.,Russian Academy of Sciences | Livanova N.N.,Russian Academy of Sciences | Panov V.V.,Russian Academy of Sciences | And 4 more authors.
Vector-Borne and Zoonotic Diseases | Year: 2011

The specimens of 3552 questing adult Ixodes persulcatus and 1698 blood/tissue samples of small mammals collected in Ural, Siberia, and Far East of Russia were assayed for the presence of Anaplasma phagocytophilum by nested PCR based on the 16S rRNA gene. Totally, A. phagocytophilum was detected in 112 tick and 88 mammalian samples. The nucleotide sequences of the 16S rRNA gene and groESL operon (1244-1295 bp) were determined for A. phagocytophilum samples from 65 ticks and 25 small mammals. Six different 16S rRNA gene variants differing by 1-5 nucleotide substitutions were detected, and only one variant matched the sequences deposited in GenBank. Analysis of groESL sequences allowed the A. phagocytophilum samples to be divided into three groups; moreover, the samples from different groups also differed in the 16S rRNA gene sequences. The A. phagocytophilum sequences from group I were detected in 11 Myodes spp. samples from West Siberia and Far East and in 19 I. persulcatus samples from all examined regions; from group II, in 10 samples of Myodes spp. and common shrews (Sorex araneus) from Ural; and from group III, in four samples of Asian chipmunks (Tamias sibiricus) from West Siberia and Far East; and in 46 I. persulcatus samples from all examined regions. The nucleotide sequences of A. phagocytophilum groESL operon from groups I and II were strictly conserved and formed with A. phagocytophilum groESL sequence from a Swiss bank vole (Myodes glareolus) (GenBank accession no. AF192796), a separate cluster on the phylogenetic tree with a strong bootstrap support. The A. phagocytophilum groESL operon sequences from group III differed from one another by 1-4 nucleotides and formed a separate branch in the cluster generated by European A. phagocytophilum strains from roe deer (Capreolus capreolus) and Ixodes ricinus ticks. © Copyright 2011, Mary Ann Liebert, Inc.

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