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Parveen I.,Aberystwyth University | Wilson T.,Aberystwyth University | Threadgill M.D.,University of Bath | Luyten J.,Kh Kempen University College | And 5 more authors.
Phytochemistry | Year: 2014

Society is demanding more green chemicals from sustainable sources. Miscanthus is a potential source of platform chemicals and bioethanol through fermentation. Miscanthus sinensis (M. sinensis) has been found to contain particularly high levels of soluble phenols (hydroxycinnamates and flavonoids) which may have application in the nutraceutical, cosmetic and pharmaceutical industries. Here, we describe the first study on the identification and quantification of phenols from the leaf tissue of a bi-parental M. sinensis mapping family. Parents and progeny showed complex profiles of phenols with highly related structures which complicated characterisation of individual phenotypes. Separation of semi-purified extracts by reverse-phase liquid chromatography, coupled with detection by diode array and ESI-MS/MS, enabled distinction of different profiles of phenols. Ten hydroxycinnamates (O-cinnamoylquinic acids) and several flavones (one mono-O-glycosyl flavone, eight mono-C-glycosyl flavones, two di-C-glycosyl flavones, five O-glycosyl-C-glycosyl flavones and nine 2″-O-glycosyl-C-glycosyl flavones) were identified and quantified in leaf tissue of two hundred progeny and maternal and paternal plants during the seedling stage. Progeny exhibiting high, moderate and low amounts of hydroxycinnamates and flavonoids and both parents were selected and screened at seven months' growth to determine the abundance of these phenols at their highest biomass and compared with seedlings. Concentrations of phenols generally decreased as leaves matured. Several flavone-glycosides were identified. This technique can be used for rapid screening of plants in a mapping family to identify genotypes with high phenol content to add value in the biorefinery chain. This comparative study provides information on the content of potentially valuable compounds from readily renewable resources and possible biomarkers for identification in breeding programmes. © 2014 Elsevier Ltd. All rights reserved. Source


Van de Vel A.,University of Antwerp | Cuppens K.,Kh Kempen University College | Cuppens K.,Future Health | Bonroy B.,Kh Kempen University College | And 8 more authors.
Epilepsy and Behavior | Year: 2013

Long-term home monitoring of epileptic seizures is not feasible with the gold standard of video/electro-encephalography (EEG) monitoring. The authors developed a system and algorithm for nocturnal hypermotor seizure detection in pediatric patients based on an accelerometer (ACM) attached to extremities. Seizure detection is done using normal movement data, meaning that the system can be installed in a new patient's room immediately as prior knowledge on the patient's seizures is not needed for the patient-specific model.In this study, the authors compared video/EEG-based seizure detection with ACM data in seven patients and found a sensitivity of 95.71% and a positive predictive value of 57.84%.The authors focused on hypermotor seizures given the availability of this seizure type in the data, the typical occurrence of these seizures during sleep, i.e., when the measurements were done, and the importance of detection of hypermotor seizures given their often refractory nature and the possible serious consequences. To our knowledge, it is the first detection system focusing on this type of seizure in pediatric patients. © 2012 Elsevier Inc.. Source


Vanheukelom V.,Kh Kempen University College | Van Beirendonck S.,Kh Kempen University College | Van Thielen J.,Kh Kempen University College | Driessen B.,Kh Kempen University College
Applied Animal Behaviour Science | Year: 2012

Surgical castration of male pigs is a practice that will probably be abandoned in Europe in the future because of general protest against pain in animals and a growing concern on (farm) animal welfare. Rearing entire males will therefore inevitably be integrated in the pig sector. In the present study, possible differences in behavior, production results and meat quality between entire males and gilts, both housed in single-sex groups but with visual contact to other groups, were investigated. The influence of the location of the pen (next to a group of the same sex or next to a group of the opposite sex) was also looked at. A total of 141 hybrid pigs - 70 entire males and 71 gilts - heterozygous for the halothane gene, were observed from 18 to 24 weeks of age. During this period, boars were more active than gilts and displayed more aggression and mounting behavior. Housing boars next to a pen with gilts increased explorative and drinking behavior. Gilts housed next to a boar pen tended to show less inactivity. Considering production results, boars tended to have a higher growth during the final phase but gilts showed better carcass traits. High skin lesion scores were more frequent in boars. © 2012 Elsevier B.V. Source

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