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El-Harairy M.A.,Mansoura University | El-Razek I.M.A.,Kfrelshiekh University | Abdel-Khalek E.A.,Mansoura University | Shamiah S.M.,Egyptian Animal Production Research Institute | And 2 more authors.
Asian Journal of Animal Sciences | Year: 2016

This study was conducted to determine the effect of different type and levels of antioxidant supplementation (0.4 and 0.8 mM from glutathione, GSH or 0.5 and 1.0 g LG-1 extender from ascorbic acid, AA) as compared to control, on characteristics of camel epididymal spermatozoa stored at 25EC (room temperature) for 0, 2, 4 and 12 h or at 5EC (cool temperature) for 0, 12, 24 and 48 h. Testis of camel were collected after animal slaughtering and placed immediately into plastic bag into ice box at 5EC. Epididymal spermatozoa were collected by aspiration from tail and extended with tris-egg yolk extender. Results of epididymal spermatozoa stored at 25EC showed improvement in livability (p<0.05) and abnormality (p$0.05) with GSH (0.4 mM), while sperm motility and curling spermatozoa improved (p$0.05) with AA (0.5 g LG-1). Storage at 5EC improved (p<0.05) motility, livability and curling spermatozoa with GSH (0.4 mM), while sperm abnormality improved (p<0.05) with AA (1 g LG-1). At different incubation times at 25 or 5EC, percentages of motility, livability and curling spermatozoa decreased (p<0.05) and of sperm abnormality increased (p<0.05) by increasing storage time. The effect of interaction between antioxidant supplementation and storage time on all sperm characteristics studied was not significant. In conclusion, supplementation of tris-egg yolk extender with GSH (0.4 mM) or AA (0.5 g LG-1) has a vital role in maintaining function, morphology and membrane integrity of epididymal camel spermatozoa stored at 25EC for 12 h or at 5EC for 48 h, respectively. © 2016 M.A. El-Harairy et al.

Kandeel M.,Gifu University | Kandeel M.,Kfrelshiekh University | Yosef T.,Kfrelshiekh University | Yosef T.,Inhalation Toxicology Research Institute | And 3 more authors.
Life Science Journal | Year: 2013

Ethylene-diaminetetraacetic acid (EDTA) is the gold standard as a chelating agent in treatment of certain diseases as well as treatment of metal poisoning. Here, we used isothermal titration calorimetry (ITC) as a simple and rapid method for detecting the stoichiometry, binding affinities and mechanism of interactions of EDTA with several toxic and biologically important cations. The aspects of this work will clarify the differences in the interactions of various cations with EDTA. Mono-, bi- and trivalent cations were titrated into EDTA solution under isothermic conditions by using ITC. Weak or no binding patterns were observed with mono- and trivalent cations. Divalent cations can be classified into two groups, high affinity cations as Ca2+, Mn2++, Co2++, Zn2++ and Pb2++and medium affinity cations as Ba2++ and Mg2++. All EDTA-bound cations showed the profiles of tight binding as favorable enthalpic and entropic terms. In contrast, Mg2++ showed a different profile by adopting unfavorable enthalpic binding conditions. ITC allows graphical display of the EDTA-cations binding events, so that, the number of cations binding with EDTA, binding affinity and mechanism of interaction can be concluded by visual examination of the binding isotherms. By ITC, we show that EDTA adapts to the binding with cations under highly variable enthalpic and entropic conditions. The ITC experiment not only determines the number of cations interacting with one molecule of EDTA, but also, we can determine the mechanisms of interaction and full thermodynamic parameters in one experiment.

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