Wageningen, Netherlands
Wageningen, Netherlands

Time filter

Source Type

Patent
Keygene N.V. | Date: 2016-12-01

The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.


Patent
Keygene N.V. | Date: 2017-03-01

The present invention provides a method for improving at least one phenotypic trait of interest in subsequent generation(s) of a population of individuals, preferably crop plants or cattle. Particularly, the method identifies the combination of at least three individuals that gives, upon subsequent intercrossing, the highest estimated probability of improving the at least one phenotypic trait of interest in the subsequent generation(s). Also provided is a computer-readable medium comprising instructions for performing the method.


Grant
Agency: European Commission | Branch: FP7 | Program: CP-IP | Phase: KBBE.2013.3.3-01 | Award Amount: 7.01M | Year: 2014

Natural rubber (NR) is an essential renewable material for more than 40,000 products essential to building (adhesives, sealants), medicine (gloves, tubing), and transportation (matting, tyres) industries. In many applications NR cannot be replaced by synthetic rubbers. At the moment NR is harvested exclusively from the rubber tree (Hevea brasiliensis) of which 90% is grown in South East Asia. Worldwide NR consumption is forecasted to increase significantly. At the moment the EU is completely dependent on imports of NR. The successful EU-PEARLS project, which finished in 2012, demonstrated the viability of a new NR producing concept: Russian dandelion (Taraxacum koksaghyz , abbreviated TKS). Not demonstrated in the EU-PEARLS project, but of equal importance in the construction of the TKS business case, is the fact that TKS is also a promising new crop for biomass derived building blocks for the chemical industry via the production of inulin. The strategic objective of EU-DRIVE is to demonstrate the economic potential of a European production chain for natural rubber and inulin as building block for green chemicals, using TKS as a dual purpose production platform. The main deliverables of DIRVE4EU are: (1) plant genotypes with high root biomass, high rubber and inulin yield, (2) seed batches for agronomic tests and large scale demo field trials, (3) optimized cultivation and harvest methods for TKS, (4) ecological analysis of the gene flow between TKS and wild dandelions, (5) scaled-up and optimised extraction and refinery protocol for TKS NR and inulin, (6) testing and application of TKS NR and inulin in end product uses, (7) demonstration of the economic viability of the TKS production chain for NR and inulin. To reach the objective a strong consortium of 8 industrial partners and 5 research organisations from 7 EU countries and Kazakhstan is constructed with a very broad background from bioscience to the rubber industry.


The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3 end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.


The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.


Patent
Keygene N.V. | Date: 2016-07-20

The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.


Patent
Keygene N.V. | Date: 2016-03-23

The invention relates to a method for the generation of unique molecular markers in breeding material, e.g. germplasm, by selecting a marker associated with a trait, identifying the existing variation at the nucleotide level within the marker in a number of members of the breeding population, preferably plants, and introducing a selectable marker by the introduction of one or more nucleotides at positions in a constant region of the marker by targeted nucleotide exchange.


Patent
Keygene N.V. | Date: 2016-05-16

The present invention provides compounds and compositions for attracting or repelling sap-sucking insects, such as whitefly, as well as methods for using such compositions.


Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.


The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3 end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.

Loading Keygene NV collaborators
Loading Keygene NV collaborators