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Liu M.M.,Zhejiang Sci-Tech University | Zheng Y.W.,Zhejiang Sci-Tech University | Wang B.,Key Scientific Research Base of Textile Conservation | Zhou Y.,Key Scientific Research Base of Textile Conservation | Hu Z.W.,Zhejiang University of Science and Technology
Advanced Materials Research | Year: 2014

In this study, an indirect ELISA was applied for specific identification of gelatin and collagen I in the binders of ancient Chinese textiles. The process consists of protein extraction and incubation, blocking, primary antibody incubation, secondary antibody incubation and coloration. To guarantee stringency, both artificially aged samples and archaeological samples were well prepared before testing. The results indicated that all the samples behaved positive for ovalbumin while negative for collagen I. This is the first time ELISA was applied in the detecting of binders of ancient textiles. Compared with other detecting methods, ELISA was sensitive, simple and specific. With such superior features, ELISA will become a frequently-used technique in the detection of proteins in ancient materials. © (2014) Trans Tech Publications, Switzerland. Source


Xie J.,Zhejiang Sci-Tech University | Jiang X.Q.,Zhejiang Sci-Tech University | Ye Y.Y.,Zhejiang Sci-Tech University | Teng H.K.,Zhejiang Sci-Tech University | And 3 more authors.
Advanced Materials Research | Year: 2014

This work was designed to evaluate the antibacterial activity of one novel leather material treated with bactericides. The growth of bacteria species such as colibacillus and staphylococcus aureus were measured by the diameter of inhibition zone. The results showed that glutaraldehyde exhibits an antibacterial activity against colibacillus and staphylococcus aureus. Besides, it also is testified that 2-Octyl-4-isothiazolin-3-one was an excellent bactericidal agents to staphylococcus aureus, instead of colibacillus. The concentrations of tea saponin and sodium above 0.05mg/L, the growth of bacteria was restrained. In addition, compared with single of bactericidal agents, the mixture of two bactericidal agents showed much better antibacterial activity. Among these antimicrobial agents, the mxture of 2-Octyl-4-isothiazolin-3-one and glutaraldehyde were of great potential in application. © (2014) Trans Tech Publications, Switzerland. Source


Yao Q.,Zhejiang Sci-Tech University | Yu N.,Zhejiang Sci-Tech University | Xu P.,Zhejiang Sci-Tech University | Zhou Y.,Key Scientific Research Base of Textile Conservation | And 3 more authors.
Applied Mechanics and Materials | Year: 2013

The humidity control composite paperboard with excellent humidity control performance was prepared, comprising sodium chloride, anhydrous potassium carbonate, diatomite, pyrrolidone carboxylic acid-Na, carboxymethyl cellulose and self-made humidity control material. The moisture absorption and desorption rate of the sample are about 0.6231 (g/7h•g-1) and 0.5852 (g/7h•g-1), respectively. The equilibrium humidity fluctuates from 52% to 56% and moisture capacity is 31%. Moreover, it can reach to the equilibrium levels within 60 minutes. Above all, it shows outstanding humidity control properties, meeting the requirement of micro-environment particularly for something sensitive to humidity such as cultural relic. © (2013) Trans Tech Publications, Switzerland. Source


Feng D.,Zhejiang Sci-Tech University | Lin Z.,Zhejiang Sci-Tech University | Liu M.,Zhejiang Sci-Tech University | Xie J.,Zhejiang Sci-Tech University | And 11 more authors.
Microporous and Mesoporous Materials | Year: 2016

Silica-alumina gel with micrometer-sized macropores and nanometer-sized mesopores has excellent advantages in humidity control properties. The macropores provide pathways for rapid H2O molecular transportation while the mesopores present a large surface area. An amorphous spherical silica-alumina beads material with a distinct bimodal pore structure was prepared by sol-gel reactions of tetraethoxysilane (TEOS) and aluminum nitrate in the presence of poly(ethyleneoxide) (PEO). It features high moisture adsorption capacity, super fast response to humidity changes, small equilibrium humidity control range and macroscopic spherical morphology. The appearance and structural properties of the material were investigated by digital camera, scanning electronic microscope (SEM) and thermal gravimetry and differential thermal analyses (TGA/DTA). The porosity features are further established by N2 sorption experiments and pore size distribution analysis. The bonding characteristics and coordination features around aluminum have been studied using Fourier transform infrared spectroscopy (FTIR) and 27Al magic angle spinning nuclear magnetic resonance (27Al MAS NMR). The humidity control performance of the material at relative humidity was measured. The results revealed that the material kept a relative humidity in the range of 39.3-40.3%, 47.6-50.7% and 57.2-60.7%, respectively, under three different pre-treating conditions. And the humidity controlling equilibrium levels were all reached within 3 h. Therefore, the material could maintain a suitable relative humidity of 40-60% in enclosed spaces such as showcases in museums and galleries. © 2015 Elsevier Inc. Source


Liu M.,Zhejiang Sci-Tech University | Xie J.,Zhejiang Sci-Tech University | Zheng H.,Key Scientific Research Base of Textile Conservation | Zhou Y.,Key Scientific Research Base of Textile Conservation | And 2 more authors.
Analytical Sciences | Year: 2015

The identification of ancient silk is of great importance in both archaeology and academia. In the present work, a specific antibody having the characteristics of low cost, easy operation and extensive applicability was developed directly through immunizing rabbits with complete antigen (silk fibroin, SF). Then, antibody-based immunoassays, i.e. enzyme-linked immunosorbent assay (ELISA) and immuno-fluorescence microscopy (IFM), were established and conducted in tandem to identify the corresponding protein in ancient silks. The anti-SF antibody exhibits high sensitivity and specificity for the identification of modern and ancient silks. The detection limit of the ELISA method is about 0.1 ng/mL, and no cross-reactions with other possible interference antigens have been noted. IFM makes it possible to localize target proteins in archaeological samples, and also ensure the reliability of the ELISA results. Based on these advantages, immunological techniques have the potential to become powerful analytical tools at archaeological sites and conservation science laboratories. © 2016 The Japan Society for Analytical Chemistry. Source

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