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Niu X.-Y.,Key Laboratory of Zoonosis Research | Niu X.-Y.,Zhengzhou University | Zhang H.-Y.,Key Laboratory of Zoonosis Research | Liu Y.-J.,Key Laboratory of Zoonosis Research | And 3 more authors.
Life Sciences | Year: 2013

Aims Ankylosing spondylitis (AS) is an autoimmune disease and is associated with abnormal B cell function. However, the roles of different B cell subsets are less clear. This study aimed to examine the frequency of different subsets of B cells in AS patients following standard therapies. Main methods Eighteen newly diagnosed AS patients and 10 healthy controls (HC) were recruited in this study. The expression of CD27, CD38, CD86, CD95 and IgD on CD19+ B cells was examined by flow cytometry. The disease activity was scored according to the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Serum levels of C-reactive protein (CRP), rheumatoid factor (RF), IgG, IgA and IgM, and the erythrocyte sedimentation rate (ESR) were measured. Key findings The frequency of CD27+ B cells was decreased in AS patients compared with HC (p = 0.018), while CD86+ and CD27-CD95+ B cell subsets increased in AS patients (p < 0.001). Meanwhile, the frequencies of CD38+ and CD95+ B cell positively correlated with BASDAI (r = 0.6505, p = 0.0035; r = 0.6854, p = 0.0017, respectively), while CD38 -CD86+ B cell negatively correlated with BASDAI (r = - 0.7329, p < 0.001). We also found that CD27- and CD95+ B cell negatively correlated with RF levels (r = - 0.5141, p = 0.0290; r = - 0.4944, p = 0.0370, respectively), while CD27+ B cell positively correlated with IgG levels (p = 0.0148, r = 0.5640). Moreover, CD86+ and CD27-CD95+ B cell subsets increased following treatment with Meloxicam and Etanercept for one month (p < 0.001; p < 0.001). Significance These findings suggest that CD27-CD95 +CD19+ and CD86+CD19+ B cells may be reasonable cellular targets for the therapeutic intervention of AS. © 2013 Elsevier Inc. Source


Li C.,Key Laboratory of Zoonosis Research | Zhao P.,Key Laboratory of Zoonosis Research | Sun X.,Key Laboratory of Zoonosis Research | Che Y.,Key Laboratory of Zoonosis Research | Jiang Y.,Key Laboratory of Zoonosis Research
Mediators of Inflammation | Year: 2013

Aims. Interleukin-37 (IL-37) is an anti-inflammatory cytokine. This study aims to investigate the concentrations of plasma and cerebrospinal fluid (CSF) IL-37 in patients with Guillain-Barré Syndrome (GBS). Methods. The levels of plasma and CSF IL-37, IL-17A, IFN-γ, and TNF-α in 25 GBS patients and 20 healthy controls (HC) were determined by enzyme-linked immunoabsorbent assay and flow cytometric bead array assay, respectively. The values of clinical parameters in the patients were also measured. Results. The concentrations of plasma IL-37, IL-17A, IFN-γ, and TNF-α and CSF IL-37 and IL-17A in patients at the acute phase of GBS were significantly higher than those in the HC. The levels of plasma IL-37, IL-17A, IFN-γ, and TNF-α were positively correlated in those patients, and the levels of CSF IL-37 and IL-17A as well as the levels of plasma TNF-α were correlated positively with the GBS disability scale scores (GDSs) in those patients. Treatment with intravenous immunoglobulin significantly reduced the levels of plasma IL-37, IL-17A, IFN-γ, and TNF-α in the drug-responding patients. Conclusions. Our findings indicate higher levels of plasma and CSF IL-37 and IL-17A and other proinflammatory cytokines in patients with GBS. © 2013 Cong Li et al. Source


Zhang H.,Key Laboratory of Zoonosis Research | Xiao F.,Key Laboratory of Zoonosis Research | Liu Y.,Key Laboratory of Zoonosis Research | Zhao D.,Key Laboratory of Zoonosis Research | And 2 more authors.
International Immunopharmacology | Year: 2014

Objectives B cells play important roles in inflammatory diseases. This study was aimed at examining the frequency of different subsets of B cells in patients with non-traumatic osteonecrosis of the femoral head (NONFH). Methods The percentages of the different subsets of circulating B cells in 28 patients with steroid-related, alcohol-related, or idiopathic NONFH and 10 healthy controls (HC) were examined by flow cytometry. The concentrations of serum C-reactive protein (CRP), fibrinogen (FIB), immunoglobulins, cytokines and blood erythrocyte sedimentation rate (ESR) were measured. Results In comparison with those in the HC, significantly higher percentages of CD27 -, CD86 +, CD95 +, and CD27 + CD95 +CD19 + but lower CD27 + CD19 + B cells were detected in the patients. The percentages of CD86 +, CD95 +, and CD27 + CD95 +CD19 + B cells in each group of the patients were significantly higher than those in the HC. The levels of serum IL-17A and IFN-γ in steroid group and serum TNF-α in alcoholic group were significantly higher than those in the HC. The percentages of CD86 + CD19 + B cells were positively associated with the degrees of femoral head collapse in both steroid and alcoholic groups of patients and the levels of serum TNF-α were positively associated with the degrees of femoral head collapse in the alcoholic NONFH patients. Conclusions These data suggest a higher frequency of CD86 + CD19 + activated B cells and elevated levels of serum TNF-α may be associated with the development of NONFH. © 2014 Elsevier B.V. Source

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