Key Laboratory of Xinjiang Endemic Phytomedicine Resources

Laboratory of, China

Key Laboratory of Xinjiang Endemic Phytomedicine Resources

Laboratory of, China
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Gong F.-K.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources | Kong Q.-J.,Shihezi University | Zhang B.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources
Chinese Journal of Pharmacology and Toxicology | Year: 2014

Viniferin is the generic term of oligomers of resveratrol, which acts as phytoalexin in Leguminosae, Polygonaceae, Vitaceae, Ranunculaceae, Dipterocarpaceae and other plants. Viniferin plays important physiological roles in protecting against UV damage and resisting bacterial fungal and viral infection in plants. Nevertheless, these oligomers have shown various pharmacological activities including antioxidative activities, anti-pathogenic, anti-inflammatory and anti-tumor activities. This paper review the recent advances in research of viniferins microbes to show their key pharmaceutical activities for pharmaceutic references.


Guo D.-D.,Shihezi University | Chen J.,Shihezi University | Yu B.-C.,Shihezi University | Zhang B.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources | Zheng Q.-S.,Shihezi University
Chinese Journal of Pharmacology and Toxicology | Year: 2013

OBJECTIVE: To evaluate the anti-metastasis effect and the inhibition of the tube-like structure formation of resveratrol and pterostilbene. METHODS: The proliferation inhibitory rate in B16F1 and ECV304 cells was determined by sulforhodamine B method. The migrating ability in vitro was determined by scratch wound assay. Expression and activity of matrix metalloproteinase-2 (MMP-2) was determined by ELISA and gelatin zymography. The inhibitory effect on ECV304 cell tube-like structure formation was observed by the test of reconstituted basement membrane. RESULTS: Resveratrol and pterostilbene (5-50 μmol·L-1) significantly inhibited the proliferation of ECV304 and B16F1 cells after 48 h treatment. The IC50 for B16F1 cells was 119.7 and 37.3 μmol·-L-1, respectively, and the IC50 for ECV304 cells was 72.2 and 37.2 μmol·L -1, respectively. After treatment with resveratrol and pterostilbene 10 μmol·L-1 for 48 h , the scratch healing rate was 58.0% and 36.8% for B16F1 cells, 61.8% and 28.9% for ECV304 cells, and 67.7% and 88.4% (P<0.01 ) for control group. The scratch healing rate of pterostilbene was significantly lower than that of resveratrol group. Compared with the normal group , after treatment by resveratrol and pterostilbene 10 umol·L -1 for 48 h, MMP-2 protein expression and MMP-2 activity was reduced (P<0.01). Tube-like structure formation of ECV304 cells was inhibited significantly by resveratrol and pterostilbene 10 μmol·L-1 for 24 h, and the inhibitory effect of pterostilbene was more obvious than that of resveratrol. CONCLUSION: Resveratrol and pterostilbene decrease proliferation and metastasis of B16F1 and CV304 cells, and inhibit tube-like structure formation of CV304 cells. At the same concentration of 10 μmol·L -1, the inhibitory effect of pterostilbene is more significant than that of resveratrol.


Caixia W.,Yantai University | Qiusheng Z.,Yantai University | Yishan W.,Shihezi University | Xiaoyu C.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources
Proceedings 2011 International Conference on Human Health and Biomedical Engineering, HHBE 2011 | Year: 2011

The aim of this study was to evaluate the anti-metastasis effects and the inhibition of the tube-like structure formation of human umbilical vein endothelial cells ECV304 of isoliquiritigenin and glabridin. The cell survival ratio of mice melanoma cells B16F1 was measured by SRB assay, the lethality rate was tested by trypan blue exclusion test. The survival ratio of human umbilical vein endothelial cells ECV304 were measured by SRB and AO/EB assays. The migration of B16F1 cells and ECV304 cells were measured by scratch wound assay. The matrix metalloproteinase-2 (MMP-2) activity in B16F1 cells culture medium was measured by gelatin zymography and ELISA methods. The inhibition on tube-like structure formation of human umbilical vein endothelial cells ECV304 were detected on artificial basilar membrane rebuilt by matrigel. Taken together, our data suggested that isoliquiritigenin and glabridin have strong anti-metastasis activities, in the same concentration, isoliquiritigenin has stronger function. © 2011 IEEE.


Yuan X.,Shihezi University | Li. D.,Shihezi University | Chen H.,Shihezi University | Sun C.,Shihezi University | And 4 more authors.
ICBBT 2010 - 2010 International Conference on Bioinformatics and Biomedical Technology | Year: 2010

Previous reports reveal that Isoliquiritigen(ISL), a licorice flavonoid, possesses various antitumour properties, cyclophosphamide, an alkylating agent, has various genotoxic and carcinogenic effects, and to be involved in some secondary neoplasmas. However, it is still used extensively as an antitumour agent and immunosuppressant in the clinic. In order to find out whether isoliquiritigen could enhance antitumour activity, as well as decrease genotoxic effects of cyclophosphamide or not, the antitumour activity and genotoxic effect of oral intake of ISL combined with intraperitoneal injection of cyclophosphamide was investigated. Mice bearing mouse sarcoma S180 cells and cervical cancer U14 cells were respectively used to estimate the antitumour activity in vivo. The clastogenic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronucleus. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results indicated that oral administration of ISL (5, 10 and 20 mg/kg body weight) alone has no obvious antitumour activity and genotoxic effect in mice, while ISL synergistically enhanced the antitumour activity of cyclophosphamide (40 mg/kg body weight) in a dose-dependent manner. in addition, ISL can decrease the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way. Our results suggested that ISL is able to enhance the antitumour activity and decrease the genotoxic effect of cyclophosphamide. © 2010 IEEE.


Bo Z.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources | Bo Z.,Shihezi University | Chen X.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources | Jing Z.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources | And 4 more authors.
Bio-Medical Materials and Engineering | Year: 2014

Non-small-cell lung cancer (NSCLC), the most common type of lung cancers, is resistant to initial chemotherapy intrinsically. The expressions of xenobiotic metabolism genes, antioxidants, and drug efflux proteins are increased in NSCLC. In addition, a redox-sensitive transcription factor named Nrf2 regulates the drug resistance via the expression of electrophile, oxidants detoxification enzymes and efflux mechanism. As was detected by real-time PCR, inhibiting Nrf2 expression through the transfection of shRNA plasmids in A549 cells significantly inhibits the expressions of glutathione pathway genes, antioxidants and multidrug resistance proteins. Using biochemical assays and free radical medical experiments in vitro, it was identified that the RNAi-mediated reduction of Nrf2 expression in lung cancer cells induces the generation of reactive oxygen species, decreases the level of reduced glutathione and results in an increase in the A549 cell proliferation inhibition rate. Thus, targeting Nrf2 activity in NSCLC could be a practical way to inhibit tumor growth and eliminate chemoresistance. © 2014 - IOS Press and the authors.


Yuan X.,Qingdao University | Niu H.-T.,Qingdao University | Wang P.-L.,Key Laboratory of Xinjiang Endemic Phytomedicine Resources | Lu J.,Qingdao University | And 5 more authors.
PLoS ONE | Year: 2015

Flavonoids are important components of 'functional foods', with beneficial effects on cardiovascular function. The present study was designed to investigate whether licochalcone D (LD) could be a cardioprotective agent in ischemia/reperfusion (I/R) injury and to shed light on its possible mechanism. Compared with the I/R group, LD treatment enhanced myocardial function (increased LVDP, dp/dtmax,dp/dtmin, HR and CR) and suppressed cardiac injury (decreased LDH, CK and myocardial infarct size). Moreover, LD treatment reversed the I/ R-induced cleavage of caspase-3 and PARP, resulting in a significant decrease in proinflammatory factors and an increase in antioxidant capacity in I/R myocardial tissue. The mechanisms underlying the antiapoptosis, antiinflammation and antioxidant effects were related to the activation of the AKT pathway and to the blockage of the NF-κB/p65 and p38 MAPK pathways in the I/R-injured heart. Additionally, LD treatment markedly activated endothelial nitric oxide synthase (eNOS) and reduced nitric oxide (NO) production. The findings indicated that LD had real cardioprotective potential and provided support for the use of LD in myocardial I/R injury. © 2015 Yuan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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