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Xie Y.,Shihezi University | Zong P.,Shihezi University | Wang W.,Shihezi University | Liu D.,Shihezi University | And 16 more authors.
Experimental and Molecular Pathology | Year: 2015

Soft tissue sarcomas (STSs) are comparatively rare malignant tumors with poor prognosis. STSs predominantly arise from mesenchymal differentiation. MicroRNA-34b/c, the transcriptional targets of tumor suppressor p53, possesses tumor suppressing property. Hypermethylation of miR-34b/c has been associated with tumorigenesis and the progression of various cancers. To determine whether aberrant miR-34b/c methylation occurs in STSs, we quantitatively evaluated the methylation level of miR-34b/c in 57 STS samples and 20 cases of peripheral blood from healthy volunteers serving as normal controls by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We found that miRNA34b/c is more frequently methylated in STSs (0.157. ±. 0.028) than in normal controls (0.098. ±. 0.012, p. =. 0.038). Furthermore, the methylation levels of CpG_1.2.3, CpG_4.5.6.7, and CpG_11.12.13 of miR-34b/c were significantly higher in the STS group than in the normal control group (p. <. 0.001). No significant differences in the methylation levels within miR-34b/c were observed between specific reciprocal translocations in STSs and nonspecific reciprocal translocations in STSs (0.146. ±. 0.039 vs. 0.168. ±. 0.035, p. >. 0.05). The methylation levels of miR-34b/c in STSs were associated with clinical stage. The methylation levels of CpG_1.2.3, CpG_4.5.6.7, CpG_9.10, CpG_11.12.13, and CpG_14 in tumor-stage III/IV tissues were significantly higher than those in tumor-stage I/II tissues. Our findings indicated that DNA hypermethylation of the miR-34b/c is a relatively common event in STSs and is significantly correlated with late clinical stage in patients with STSs. Hypermethylation of the miR-34b/c may be pivotal in the oncogenesis and progression of STSs and may be a potential prognostic factor for STSs. © 2015.

Zou H.,Huazhong University of Science and Technology | Zou H.,Shihezi University | Zou H.,Key Laboratory of Xinjiang Endemic and Ethnic Diseases | Kang X.,Fudan University | And 18 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014

To study the clinicopathological and genomic characteristics of Xp11.2 translocation renal cell carcinoma (Xp11.2 RCC) in adults, we analyzed 9 Xp11.2 RCCs, confirmed by transcription factor E3 (TFE3) immunohistochemistry, in patients aged ≥20 years. TFE3 expression was also determined in 12 cases of alveolar soft part sarcoma (ASPS) served as a positive control. Comparative genomic hybridization (CGH) was used to investigate genomic imbalances in all Xp11.2 RCC cases. Most of our Xp11.2 RCC patients (5/9) presented with TNM stages 3-4, and 6 patients died 10 months to 7 years after their operation. Histologically, Xp11.2 RCC was composed of a mixed papillary nested/alveolar growth pattern (8/9). Immunostaining showed that all Xp11.2 RCC and ASPS cases had strong TFE3 expression and high positive ratios for p53 and vimentin. However, there were significant differences in the expression of AMACR (p<0.001), AE1/AE3 (p=0.002), and CD10 (p=0.024) between the 2 diseases. CGH profiles showed chromosomal imbalances in all 9 Xp11.2 RCC cases; gains were observed in chromosomes Xp11 (6/9), 7q20-25, 12q25-31 (5/9), 7p16-24 (4/9), 8p12-13, 8q20-21, 16q20-22, 17q25-26, 20q22-23 (4/9), and losses occurred frequently on chromosomes 3p12-16, 9q31-32, 14q22-24 (4/9). Our Conclusions show Xp11.2 RCC that occur in adults may be aggressive cancers, the expressions of AMACR, CD10, AE1/AE3 are helpful in the differential diagnosis between Xp11.2 RCC and ASPS, and CGH assay is a useful complementary method for confirming the diagnosis of Xp11.2 RCC.

Wang A.,Key Laboratory of Xinjiang Endemic and Ethnic Diseases | Zhang H.,Key Laboratory of Xinjiang Endemic and Ethnic Diseases | Zhang L.,Key Laboratory of Xinjiang Endemic and Ethnic Diseases | Li X.,Key Laboratory of Xinjiang Endemic and Ethnic Diseases | And 3 more authors.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2016

OBJECTIVE: To analyze the relationships of connexin 40 (Cx40) with peripheral blood CD4(+) and CD8(+) T lymphocyte subsets and inflammatory factors of spontaneously hypertensive (SH) rats.METHODS: Flow cytometry was used to detect CD4(+), CD8(+) lymphocytes and Cx40 expression on the cells in the peripheral blood of Wistar-Kyoto (WKy) rats and SH rats. ELISA was performed to test the levels of interleukin 2 (IL-2), interferon γ (IFN-γ), IL-4 and IL-6.RESULTS: Compared with WKy rats, the systolic blood pressure, the percentage of CD4(+) T lymphocytes, the expression of Cx40 on the surface of CD4(+) and CD8(+) T lymphocytes and the ratio of CD4(+)/CD8(+) in the peripheral blood of SH rats were significantly higher, with the exception of the percentage of CD8(+) lymphocytes which was lower. Also, we found that the serum levels of IL-2, IL-4 and IL-6 in the SH rats were significantly higher than those of WKy rats. However, there was no significant difference in the IFN-γ level between SH and WKy rats.CONCLUSION: The ratio of peripheral blood CD4(+)/CD8(+) T lymphocytes and the levels of Cx40, IL-2, IL-4 and IL-6 are significantly elevated in SH rats.

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