Key Laboratory of Wildlife Conservation

Harbin, China

Key Laboratory of Wildlife Conservation

Harbin, China
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Hongliang C.,Northeast Forestry University | Hongliang C.,Key Laboratory of Wildlife Conservation | Siyuan Y.,Northeast Forestry University | Zhijun H.,Northeast Forestry University | And 4 more authors.
Pakistan Journal of Zoology | Year: 2011

In 2004, an H4N6 subtype avian influenza virus (AIV) was isolated from mallard ducks (Anas platyrhynchos) in Zhalong Nature Reserve of Heilongjiang Province in China. The virus was named A/Mallard/Zhalong/88/04(H4N6) (MZL88/04). In this study, eight gene segments of MZL88/04 were amplified by reverse transcription polymerase chain reaction (RT-PCR). Nucleotide and deduced amino acid sequence homology and phylogenetic relationships were determined between MZL88/04 and other H4N6 subtype isolates. Isolate MZL88/04 was classified into the Eurasian lineage, and was closely related to isolates from Nanchang, indicating close evolutionary relationships between the genes studied. To our knowledge, this is the first report of an H4N6 subtype AIV strain isolated from wild birds in the mainland of China. Copyright 2011 Zoological Society of Pakistan.


Hou Z.,Northeast Forestry University | Hou Z.,Key Laboratory of Wildlife Conservation | Shen M.,Northeast Forestry University | Chai H.,Northeast Forestry University | And 4 more authors.
Asian Journal of Animal and Veterinary Advances | Year: 2012

While the etiology of White Nose Disease (WND) is still uncertain, trace elements have been postulated to play a role. The purpose of this study is to determine the concentrations of Fe, Cu, Zn and Se in the liver, heart, lung, spleen, kidney and muscle of WND raccoon dogs and clarify whether changes of those trace elements are related to the disease. We measured concentrations of these trace elements in various organs in both WND and WND-free (control) groups, using flame atomic absorption spectroscopy for Fe, Cu and Zn and fluorometric method with 2,3-diaminonaphthalene for Se. Compared with the controls, the concentrations of liver, lung and muscle Fe were significantly lower in the WND group (p<0.05). Copper concentration in the lung was also significantly lower (p<0.05) in the WND group, but the levels in the spleen and muscle were significantly higher (p<0.05). While the spleen and lung levels of Zn were significantly lower (p<0.05) in the of WND group, the heart level was significantly higher (p<0.05). In comparison with controls, the lung, kidney and muscle levels of Se in the WND group were significantly lower. The correlations of the different trace element concentrations were statistically analyzed between each pair of tissues. The most frequent correlations were found for Cu-Zn within some tissues, especially in the lung. Interestingly, no correlation exists between metal concentrations was found in the liver. Abnormalities in the contents of the selected trace elements were observed, which revealed some imbalances in the inter-element relations in some organs of the WND infected raccoon dogs. These results suggest that t trace element contents and their homeostasis are disturbed in WND infected raccoon dogs. The WND raccoon dogs were either deficient in iron or in selenium, or deficient in both. © 2012 Academic Journals Inc.


Shi R.,Northeast Forestry University | Shi R.,Heilongjiang Vocational College for Nationalities | Sun Y.,Northeast Forestry University | Teng L.,Northeast Forestry University | And 3 more authors.
Mitochondrial DNA | Year: 2015

Naemorhedus baileyi belongs to the genera Naemorhedus within the subfamily Caprinae. Because of small population, narrow distribution and difficult sampling, the taxonomic status of red goral in Caprinae is still not clear. We extracted the sample DNA and amplified whole mtDNA genome with different primers. We downloaded other gene sequences of Caprinae from GenBank. All sequences included 24 species of 11 genera. Phylogenetic analyses confirmed that the subfamily Caprinae is not monophyletic, bootstrap support for this cluster is high (100%). © 2015 Taylor & Francis.


Li H.,Northeast Forestry University | Shen M.,Inner Mongolia University | Hou Z.,Key Laboratory of Wildlife Conservation | Yin X.,Inner Mongolia University
Pakistan Journal of Zoology | Year: 2016

Although Eimeria diseases common in rabbits in China, no information was available on it in Heilongjang Province. In this study, at least eight Eimeria species were detected in domestic rabbits in Harbin in Heilongjang Province. The diagnosis of Eimeria species has been based on the morphology unsporulated oocysts as well as analysis of PCR product obtained by specific primers. Copyright © 2016 Zoological Society of Pakistan.

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