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Zhang C.,Beijing Academy of Agriculture and Forestry Sciences | Gao D.,Northeast Agricultural University | Ma Y.,Key Laboratory of Biology | Zhao X.,Key Laboratory of Urban Agriculture North
Journal of Food Science | Year: 2013

The gelatin was added into pullulan films to improve their performances and lower their cost. The gelatin addition raised the tensile strength of the gelatin-pullulan composite films, and reduced the oxygen permeability. The cost of composite films was reduced comparing to that of the pullulan films. Moreover, the molecular interaction of the composite film was evaluated. The interactions of gelatin and pullulan in the composite films were detected, such as (1) formation of 2 glycosylated proteins; (2) improvement of β-sheet content; (3) formation of the interchain hydrogen bond and a semicrystalline region. Therefore, the molecular interaction was the main reason for the performance improvement of the composite films. © 2013 Institute of Food Technologists®.

Wu Y.,Shanxi Normal University | Wu Y.,Beijing Academy of Agriculture and Forestry Sciences | Liu L.,Beijing Academy of Agriculture and Forestry Sciences | Liu L.,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops North China | And 5 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2014

In this research, we have optimized AFLP reaction system for Spinacia oleracea L. High quality genomic DNA have been isolated by modified method of CTAB, and the critical process of AFLP have been optimized. The AFLP reaction system established in Spinacia oleracea L was as follows: template DNA 150 ng, digestion 37°C for 5 h, ligation 16°C for 6 h and digestion-ligation finished by single step; 0.2 mmol/L dNTPs, 1.5 mmol/L Mg2+ in pre-amplification system; pre-amplification products has been diluted 200 times used for selection amplification system. Using the optimized system, 20 pairs of E+ANN/M+ANN primers combination were screened out from 256 pairs of AFLP primers. The present results provided a strong foundation for the further study of population genetic diversity and germplasm identify of Spinacia oleracea L by AFLP method.

Li Y.M.,Beijing Academy of Agricultural and Forest Science | Li Y.M.,CAS Institute of Geographical Sciences and Natural Resources Research | Li Y.M.,Key Laboratory of Urban Agriculture North | Zhou X.Y.,CAS Institute of Geographical Sciences and Natural Resources Research | Yang J.,CAS Institute of Geographical Sciences and Natural Resources Research
Applied Mechanics and Materials | Year: 2014

We investigated soil physiochemical properties and heavy metal contamination characteristics in steel and chemical sites, aimed to provide theoretical basis for future site remediation. Results indicated that: soils in both sites showed sandy and alkaline trend. Soil total phosphorus, total potassium, and available nitrogen content in steel site, and total and available nitrogen, phosphorus and potassium content in chemical site were lower than that in farmland soil. Soil lead and zinc contamination in steel site and soil arsenic contamination in chemical site should be given priority in remediation. In addition, cadmium and arsenic risk in steel site and mercury risk in chemical site should also be paid sufficient attention. © (2014) Trans Tech Publications, Switzerland.

Wang S.,Beijing Academy of Agriculture and Forestry Sciences | Wang S.,Key Laboratory of Urban Agriculture North | Xu F.,Beijing Academy of Agriculture and Forestry Sciences | Li Z.,Yunnan Academy of Scientific and Technical Information | And 6 more authors.
Scientia Horticulturae | Year: 2015

In this study, experiments were conducted to assess the viability of cultivating Pleurotus ostreatus on a substrate containing different proportions of spent mushroom substrates (SMS) of industrial Hypsizigus marmoreus. The SMS were utilized to substitute for cottonseed hulls as the substrate for P. ostreatus cultivation. Eight treatments with the identical C/N ratio were designed. Mycelial growth rate, days required for fully colonized mycelia bags, days for primordia formation, yield, biological efficiency, and chemical biomass composition of the fruiting bodies were evaluated. Results indicated that treatment with 88% SMS had significantly fastest mycelial growth compared with the other treatments, followed by treatment with 75% SMS. It took approximately 4-5 days longer for treatment with 75 and 88% SMS to form the primordia than that of the control. With respect to fructification, the biological efficiency of the control and treatment with 12, 25, 38, 50, 62, 75, and 88% SMS for three flushes was 54.27, 61.26, 57.15, 52.90, 52.48, 48.88, 39.65, and 35.87%, respectively. Treatment with 12 and 25% SMS showed better quality traits and chemical content compared with the other five SMS supplementation treatments, while treatment with 12 and 25% SMS showed significant differences compared with the control. Therefore, the optimal industrial H. marmoreus waste supplementation for traditional cultivation of P. ostreatus was 12-25%, which could provide an economically acceptable production alterative, decreasing of costs for mushroom cultivation and reducing of the disposal problems caused by SMS. © 2015.

Xu F.,Beijing Academy of Agriculture and Forestry Sciences | Xu F.,Key Laboratory of Urban Agriculture North | Li Z.,Yunnan Academy of Scientific and Technical Information | Liu Y.,Beijing Academy of Agriculture and Forestry Sciences | And 4 more authors.
Saudi Journal of Biological Sciences | Year: 2015

In this study, the mycelial growth rate, mycelial colonization time, yield, and biological efficiency of the edible mushroom Oudemansiella canarii were determined, and the effects of different substrate combinations on productivity, chemical contents and amino acids were evaluated. Lignocellulosic wastes, such as cottonseed hull, sawdust, corncob, and their combinations supplemented with 18% wheat bran and 2% lime, were used for the cultivation of O. canarii. The biological efficiency (BE) and essential amino acid content of treatment T1, which consisted of 80% cottonseed hull, were the highest among all the tested treatments. Mixtures that included sawdust, such as treatments T2 (80% sawdust), T4 (40% sawdust + 40% cottonseed hull), and T6 (40% sawdust + 40% corncob), exhibited lower yield and BE. Corncob was good for O. canarii production in terms of yield and BE, whereas the mycelial growth rate and colonization time were lower compared to those on other substrates. Comparing the BE, essential amino acids, and other traits of the six treatments, treatment T1 (80% cottonseed hull) was the best formula for O. canarii cultivation and should be extended in the future. © 2015 The Authors.

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