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Sui J.-L.,Laboratory of Tropical Microbial Resource | Sui J.-L.,Key Laboratory of Tropical Crop Biotechnology | Sui J.-L.,Chinese Academy of Sciences | Sui J.-L.,Hainan University | And 14 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

A novel strain, 219820 T, whose metabolites were found to be active against tumour cells, was isolated and characterized. The isolate belonged to the genus Streptomyces and had white to grey aerial mycelium and long chains of smooth spores in the aerial mycelium. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 219820T had highest similarity to members of the genus Streptomyces and was most closely, albeit loosely, associated with Streptomyces crystallinus NBRC 15401 T (98.624% similarity), Streptomyces melanogenes NBRC 12890T (98.565%) and Streptomyces noboritoensis NBRC 13065T (98.564%). However, DNA-DNA relatedness and phenotypic data readily distinguished strain 219820 T from these phylogenetically related type strains. It is evident from the combination of genotypic and phenotypic data that strain 219820 T represents a novel species of the genus Streptomyces, for which the name Streptomyces sanyensis sp. nov. is proposed; the type strain is 219820 T (5CGMCC 4.5626 T 5DSM 42014 T). © 2011 IUMS.


Liu J.,Key Laboratory of Tropical Crop Biotechnology | Liu L.,Key Laboratory of Tropical Crop Biotechnology | Li Y.,Chinese Academy of Sciences | Jia C.,Key Laboratory of Tropical Crop Biotechnology | And 6 more authors.
Physiologia Plantarum | Year: 2015

MADS-box transcription factors play important roles in organ development. In plants, most studies on MADS-box genes have mainly focused on flower development and only a few concerned fruit development and ripening. A new MADS-box gene named MaMADS7 was isolated from banana fruit by rapid amplification of cDNA ends (RACE) based on a MADS-box fragment obtained from a banana suppression subtractive hybridization (SSH) cDNA library. MaMADS7 is an AGAMOUS-like MADS-box gene that is preferentially expressed in the ovaries and fruits and in tobacco its protein product localizes to the nucleus. This study found that MaMADS7 expression can be induced by exogenous ethylene. Ectopic expression of MaMADS7 in tomato resulted in broad ripening phenotypes. The expression levels of seven ripening and quality-related genes, ACO1, ACS2, E4, E8, PG, CNR and PSY1 in MaMADS7 transgenic tomato fruits were greatly increased while the expression of the AG-like MADS-box gene TAGL1 was suppressed. Compared with the control, the contents of β-carotene, lycopene, ascorbic acid and organic acid in transformed tomato fruits were increased, while the contents of glucose and fructose were slightly decreased. MaMADS7 interacted with banana 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene 1 (MaACO1) and tomato phytoene synthase gene (LePSY1) promoters. Our results indicated that MaMADS7 plays an important role in initiating endogenous ethylene biosynthesis and fruit ripening. © 2015 Scandinavian Plant Physiology Society.


Liu J.,Key Laboratory of Tropical Crop Biotechnology | Liu L.,Key Laboratory of Tropical Crop Biotechnology | Li Y.,Chinese Academy of Agricultural Sciences | Jia C.,Key Laboratory of Tropical Crop Biotechnology | And 6 more authors.
Physiologia Plantarum | Year: 2015

MADS-box transcription factors play important roles in organ development. In plants, most studies on MADS-box genes have mainly focused on flower development and only a few concerned fruit development and ripening. A new MADS-box gene named MaMADS7 was isolated from banana fruit by rapid amplification of cDNA ends (RACE) based on a MADS-box fragment obtained from a banana suppression subtractive hybridization (SSH) cDNA library. MaMADS7 is an AGAMOUS-like MADS-box gene that is preferentially expressed in the ovaries and fruits and in tobacco its protein product localizes to the nucleus. This study found that MaMADS7 expression can be induced by exogenous ethylene. Ectopic expression of MaMADS7 in tomato resulted in broad ripening phenotypes. The expression levels of seven ripening and quality-related genes, ACO1, ACS2, E4, E8, PG, CNR and PSY1 in MaMADS7 transgenic tomato fruits were greatly increased while the expression of the AG-like MADS-box gene TAGL1 was suppressed. Compared with the control, the contents of β-carotene, lycopene, ascorbic acid and organic acid in transformed tomato fruits were increased, while the contents of glucose and fructose were slightly decreased. MaMADS7 interacted with banana 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene 1 (MaACO1) and tomato phytoene synthase gene (LePSY1) promoters. Our results indicated that MaMADS7 plays an important role in initiating endogenous ethylene biosynthesis and fruit ripening. © 2015 Scandinavian Plant Physiology Society.


Liu J.-H.,Key Laboratory of Tropical Crop Biotechnology | Li Y.-C.,Key Laboratory of Tropical Crop Biotechnology | Zhang J.,Key Laboratory of Tropical Crop Biotechnology | Gao P.-Z.,Huazhong Agricultural University | And 4 more authors.
Biologia Plantarum | Year: 2016

Plant translation elongation factor 1 alpha (EF1A) is both a protein synthesis factor and an important component of plant signal transduction, immune responses, protein trafficking, and apoptosis. However, its role in plant growth and development remains unclear. Herein, a full-length EF1A gene was isolated from banana (Musa acuminata L.) fruit and termed MaEF1A. We found that MaEF1A shared high sequence identify with respective genes in other plants and the deduced amino acid sequence contained conserved regions of GTP-EFTU, GTP-EFTU-02, and GTP-EFTU-03, as well as two tRNA binding domains and six GTP-binding sites, which represent functional domains for protein biosynthesis. MaEF1A protein is mainly localized to the nucleus. MaEF1A was constitutively expressed in different banana organs including developing fruits, and the highest expression was detected in ovary 4 (Ov4) stage. Arabidopsis thaliana L. (ecotype Columbia) was transformed with MaEF1A and four transgenic lines were obtained. Three transgenic lines were selected for further phenotypic analyses. Our findings indicated that overexpressed MaEF1A could greatly enhance plant height, root length, and both rhachis and silique length by promoting cell expansion and elongation. These experiments suggested an important role for MaEF1A in plant growth and development. © 2016 Springer Science+Business Media Dordrecht

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