Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration

Beijing, China

Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration

Beijing, China

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Huang Q.,Chinese Academy of Forestry | Huang Q.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | Ding C.,Chinese Academy of Forestry | Ding C.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | And 10 more authors.
Journal of Forestry Research | Year: 2016

Molecular biological research into wood development and formation has been the focus in recent years, but the pace of discovery of related genes and their functions in the control of wood properties has been slow. The microarray technique—with its advantages of high throughput capacity, sensitivity, and reliability over other tools developed for investigating genes expression patterns—is capable of rapidly assaying thousands of genes. In this study, a cDNA microarray prepared from two cDNA libraries of developing poplar xylem tissues was used to assay gene expression patterns in immature xylem tissues at different heights from the main stem of Populus deltoides (15 years old), which was confirmed to have distinct wood properties (microfibrillar angle, woody density) by X-ray. Two hundred seventy-four transcripts with differential expression profiles between the chips were screened out, and the individual clones were subjected to 5′ sequencing. Using bioinformatic analysis, we identified candidate genes that may influence poplar wood properties, many of which belong to various regulatory and signal transduction gene families, such as zinc finger protein transcription factor, DNA-binding transcription factor, ethylene response factors, and so on. The results suggest that these genes may regulate enzymes involved in wood formation. Further work will be performed to clone these genes and determine how they influence poplar wood properties. © 2016 Northeast Forestry University and Springer-Verlag Berlin Heidelberg


Wang Y.-G.,Chinese Academy of Forestry | Wang Y.-G.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | Yang X.-H.,Chinese Academy of Forestry | Yang X.-H.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | And 2 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

The western Ordos Plateau is a distribution center of many endangered shrub species, which have suffered sand burial to various degrees during the last few years. According to the survey data of spatial point pattern of shrub communities in the arid desert of western Ordos Plateau in 2006, we investigated the structure and spatial pattern of shrub communities under different levels of sand burial to reveal its effects on shrub communities in this area. Three indices of spatial pattern based on the nearest neighbor distance, i. e. the F(t), G(t) and J(t) functions, were selected. The results indicate that sand burial is an important factor causing the extinction of sand intolerant shrubs. The number of sand intolerant shrubs gradually reduced with an increase in the depth of sand burial. For sand tolerant shrubs, shallow sand burial promoted their germination and growth, but this positive role changed to a negative effect when the depth of sand burial exceeded a specific value, showing at first an increase in the number of sand tolerant shrubs and then a decrease. Both sand tolerant and intolerant shrubs showed a random distribution pattern on a small scale under various levels of sand burial, with a significantly negative correlation under shallow sand burial. When the depth of sand burial exceeded 12 cm, the shrubs appeared to be sparsely distributed and without any significant correlation. Sand burial is an important driving force in community degradation, which tends to cause the singleness and aging of shrub species in the community and difficulty to regenerate.


Zhu W.,Chinese Academy of Forestry | Zhu W.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | Chu Y.,Chinese Academy of Forestry | Chu Y.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | And 10 more authors.
Journal of Forestry Research | Year: 2015

Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified (GM) plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16S rRNA gene to identify changes in bacterial diversity and community structure in two GM lines (D520 and D521), one non-genetically modified (non-GM) line and in uncultivated soil. After chimera filtering, 468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups (with >1 % of all sequences) across the samples. 241 of 551 genera (representing a ratio of 97.33 %) were common to all samples. A Venn diagram showed that 1.926 operational taxonomic units (OTUs) were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere. © 2015 Northeast Forestry University and Springer-Verlag Berlin Heidelberg


Zhang W.,Chinese Academy of Forestry | Zhang W.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | Chu Y.,Chinese Academy of Forestry | Chu Y.,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration | And 11 more authors.
BMC Genetics | Year: 2014

Background: Transgenic poplar (Populus × euramericana 'Guariento') plants harboring five exogenous, stress-related genes exhibit increased tolerance to multiple stresses including drought, salt, waterlogging, and insect feeding, but the complex mechanisms underlying stress tolerance in these plants have not been elucidated. Here, we analyzed the differences in the transcriptomes of the transgenic poplar line D5-20 and the non-transgenic line D5-0 using high-throughput transcriptome sequencing techniques and elucidated the functions of the differentially expressed genes using various functional annotation methods. Results: We generated 11.80 Gb of sequencing data containing 63, 430, 901 sequences, with an average length of 200 bp. The processed sequences were mapped to reference genome sequences of Populus trichocarpa. An average of 62.30% and 61.48% sequences could be aligned with the reference genomes for D5-20 and D5-0, respectively. We detected 11,352 (D5-20) and 11,372 expressed genes (D5-0), 7,624 (56.61%; D5-20) and 7,453 (65.54%; D5-0) of which could be functionally annotated. A total of 782 differentially expressed genes in D5-20 were identified compared with D5-0, including 628 up-regulated and 154 down-regulated genes. In addition, 196 genes with putative functions related to stress responses were also annotated. Gene Ontology (GO) analysis revealed that 346 differentially expressed genes are mainly involved in 67 biological functions, such as DNA binding and nucleus. KEGG annotation revealed that 36 genes (21 up-regulated and 15 down-regulated) were enriched in 51 biological pathways, 9 of which are linked to glucose metabolism. KOG functional classification revealed that 475 genes were enriched in 23 types of KOG functions. Conclusion: These results suggest that the transferred exogenous genes altered the expression of stress (biotic and abiotic) response genes, which were distributed in different metabolic pathways and were linked to some extent. Our results provide a theoretic basis for investigating the functional mechanisms of exogenous genes in transgenic plants. © 2014 Zhang et al.

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