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Zhang T.,Southern Medical University | Zhang T.,General Hospital of Guangzhou Military Command | Zhang T.,Key Laboratory of Trauma and Tissue Repair of Tropical Area | Wu X.-N.,Southern Medical University | And 13 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Whether the biological behavior and early adhesion of osteoblasts can be affected by magnesium alloy is still unknown. OBJECTIVE: To study surface properties of magnesium alloy AZ31B and to investigate the effects on the adhesion of osteoblasts in vitro. METHODS: Scanning electron microscope and energy dispersive spectrometer were used to study surface morphology and elemental composition of the magnesium alloy AZ31B. Protein absorption assay was applied to study the ability of magnesium alloy AZ31B and titanium alloy for protein adsorption. Then, mouse osteoblasts MC3T3-El were cocultured with magnesium alloy AZ31B or titanium alloy in 24-well plates to observe the cell adhesion at 2, 6 and 24 hours. RESULTS AND CONCLUSION: The surface of AZ31B was rough which is beneficial for cell adhesion. Energy dispersive spectrometer results proved that the main elements of AZ31B were magnesium, aluminum, and zinc, among which, magnesium accounted for about 96%, aluminum for about 3%, and zinc for about 1%. There were also some other elements, but the content was little. Protein adsorption experiment results showed that AZ31B had good protein adsorption capacity. When the cells were cultured 2 hours, there was no significant difference between magnesium alloy AZ31B and titanium alloy in the rate of cell adhesion, but at 6 and 24 hour, the adhesion rate of magnesium alloy AZ31B was significantly lower than that of the titanium alloy (P < 0.01). The cells cultured on the surface of AZ31B expanded, and appeared with irregular shape, mostly fusiform, and more processes. Interconnected processes were seen among some cells. These show that the magnesium alloy AZ31B has a good capacity of cell adhesion, which is suitable for early osteoblast adhesion.

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