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Zhou Z.,Key Laboratory of Translational Cancer Medicine of Fujian Province | Zhou Z.,Fujian Medical University | Liu S.,Key Laboratory of Translational Cancer Medicine of Fujian Province | Liu S.,Fujian Provincial Tumor Hospital | And 8 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To explore the expressions of receptors before and after NK cell amplification from patients with esophageal cancer and its cytotoxicity to tumor cells. Methods: Peripheral blood was collected from the Fujian Provincial Tumor Hospital, including 20 cases of esophageal cancer patients and 10 cases of healthy donors (control group). NK cells were amplified by combination of IL-2 + IL-12 + IL-15 + IL-18. Cell immunophenotype and NK cell receptor expressions (CD56+, CD69+,NKG2D, NKp30, NKp44, NKp46, CD158b and CD159a) were determined by flow cytometry. The cytotoxicity of NK cells to various tumor cell lines (K562, Raji, Eca-109 and TE-1) were detected by lactate dehydrogenase (LDH) assay. Results: Compared with the control group, the ratio of CD3 +, CD4 + and CD4+/CD8+T cells were significantly lower in the peripheral blood of patients with esophageal cancer (P < 0. 05), and the ratios of NK cells (CD56 +) and regulatory T cells (Treg) were significantly higher (P < 0. 05). The ratio of NK cells (CD3 ~ CD56 +) increased up to 90% after being cultured in combination of IL-2 + IL-12 + IL-15 + IL-18 for 20 days. N cell count expanded up to 1 000 times (P <0.01). The ratios of CD3, CD4 T cells, B cells (CD19), monocyte-macrophage cells (CD14) and regulatory T cells (T-reg) were in a significant reduction 20 days after culture (P <0. 01), with no significant difference between the patients with esophageal cancer and the control group (P >0.05). CD69 and NK cell activating receptors (NKG2D, NKp30, NKp44, NKp46) were significantly increased and the inhibitory receptors (CD158b, CD159a) were significantly decreased (P <0. 05). NK cells derived from patients with esophageal cancer showed significant increase of cytotoxicity on tumor cells K562, Raji, Eca-109 and TE-1 after culture for 20 days compared to those before culture ([69. 2 ± 5. 1 J% vs [42. 3 ± 3.0]%, [44.6 ±3.2]% vs [21.1 ±2.0]%, [69.7 ±3.9]% vs [50. 3 ±3.5]%, [67. 1 ±4.5]% vs [41. 2 ±3. 3]%, P <0. 01). Conclusion: The combined cytokines of IL-2 + IL-12 + IL-15 + IL-18 can effectively expand peripheral blood NK cells, up-regulate the expressions of activated receptor and down-regulate the expression of inhibitory receptors. The numbers and functions of the cultured NK cells can both meet the clinical treatment needs.


Ma L.,Fujian Medical University | Wu T.,Fujian Medical University | Pan J.,Fujian Medical University | Pan J.,Key Laboratory of Translational Cancer Medicine of Fujian Province | And 7 more authors.
Nutrition and Cancer | Year: 2014

The purpose of this study was to compare the changing tendency of nutrition with 54 nasopharyngeal carcinoma patients during intensity-modulated radiation therapy (IMRT), and to investigate the correlation between comprehensive nutritional status and quality of life (QoL), which was assessed by the European Organization for Research and Treatment of Cancer Core Quality-of-Life Questionnaire. The nutritional index, including body mass index, ideal body weight percentage, usual body weight percentage, albumin, hemoglobin, and total lymphocyte count (TLC), was evaluated at 2 time points: within 48 h after admission (T1) and at the end of treatment with IMRT (T2). A statistically significant downgrade of every index was observed during IMRT. A comprehensive nutritional model was established by principal components analysis at T2. QoL scores of functional (P = 0.002) and the global QoL scales (P = 0.001) existed a positive correlation with comprehensive nutritional status. QoL scores of symptom scales (P = 0.002) and 6 single items (P = 0.005) had a negative correlation with it. The scores of global QoL scales in comprehensive nutrition of normal (20.4%), moderate (55.6%), and severe malnutrition (24.1%) were 69.70 ± 17.98, 48.33 ± 19.25, and 37.18 ± 24.67, respectively. Patients with different nutritional status had different QoL (B = 10.405, SE = 2.828, t = 3.680, P = 0.001). Multiaspect nutritional supports should be enhanced to improve patients comprehensive nutritional status during treatment. © 2014 Copyright Taylor and Francis Group, LLC.

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