Mu J.,Tobacco Research Institute |
Mu J.,Chinese Academy of Agricultural Sciences |
Qian Y.,Tobacco Research Institute |
Ren M.,Key Laboratory of Tobacco Quality Control |
And 5 more authors.
Acta Tabacaria Sinica | Year: 2013
Molecular markers closely linked with resistance genes were explored with SSR technology. Population F2 was constructed from resistant variety Taiyan 7 as female parent and susceptible variety NC89 as male parent. An artificial inoculation method was adopted to test degree of the resistance to Erysiphe cichoracearum DC of their F2 family. 61 polymorphic markers obtained by screening 2317 SSR primers in 24 linkage groups were used to analyze F2 population. QTL analysis on powdery mildew resistance was conducted by WinQTLCart 2.5 software. One QTL was located on the 14th linkage group, and tightly linked with maker 52725 with genetic distance of 1.01 cM. The additive effect of QTL was -14.38 and 21.02% of phenotypic variation can be explained by QTL.