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Xin Z.,Chinese Academy of Agricultural Sciences | Xin Z.,Key Laboratory of Tea Biology and Resources Utilization | Zhang Z.,Chinese Academy of Agricultural Sciences | Chen Z.,Chinese Academy of Agricultural Sciences | And 3 more authors.
Journal of Plant Research | Year: 2014

We investigated the effect of the SHAM treatment of tea plants on their induced defense on a tea geometrid (TG), Ectropis obliqua Prout. Treatment of tea leaves with SHAM reduced the performance of TG and TG-elicited level of the lipoxygenase gene CsiLOX1 and the putative allene oxide synthase gene CsiAOS1. The release of wound-induced green leaf volatiles (GLVs) and the expression of the hydroperoxide lyase (HPL) gene CsiHPL1 were also reduced by SHAM treatment. The negative effect of SHAM dramatically reduced the total hebivore-induced plant volatiles (HIPVs) and the attractiveness to the parasitoid wasp Apanteles sp. These results indicated that SHAM may negatively mediate tea defense response against TG by modulating the wound-induced emission of GLVs, the expression of genes involved in oxylipin pathway, and the emission of other HIPV compounds that mediate direct and indirect defenses. © 2014 The Botanical Society of Japan and Springer Japan. Source


Sun X.-L.,Chinese Academy of Agricultural Sciences | Sun X.-L.,Key Laboratory of Tea Biology and Resources Utilization | Wang G.-C.,Chinese Academy of Agricultural Sciences | Wang G.-C.,Henan Institute of Science and Technology | And 9 more authors.
Journal of Chemical Ecology | Year: 2014

Herbivore-induced plant volatiles have been reported to play a role in the host-searching behavior of herbivores. However, next to nothing is known about the effect of volatiles emitted from tea plants infested by Ectropis obliqua larvae on the behavior of conspecific adults. Here, we found that tea plants infested by E. obliqua caterpillars for 24 h were more attractive to both virgin male and female E. obliqua adults than were intact, uninfested tea plants; moreover, mated female E. obliqua moths were more attracted by infested tea plants and preferentially oviposited on these plants, whereas male moths were repelled by infested plants once they had mated. Volatile analysis revealed that the herbivore infestation dramatically increased the emission of volatiles. Among these volatiles, 17 compounds elicited antennal responses from both male and female virginal moths. Using a Y-tube olfactometer, we found that 3 of the 17 chemicals, benzyl alcohol, (Z)-3-hexenyl hexanoate, and (Z)-3-hexenal, were attractive, but two compounds, linalool and benzyl nitril, were repellent to virgin male and female moths. One chemical, (Z)-3-hexenyl acetate, was attractive only to virgin males. Mated females were attracted by three compounds, (Z)-3-hexenyl hexanoate, (Z)-3-hexenyl acetate, and (Z)-3-hexenal; whereas mated males were repelled by (Z)-3-hexenol. The findings provide new insights into the interaction between tea plants and the herbivores, and may help scientists develop new measures with which to control E. obliqua. © 2014, Springer Science+Business Media New York. Source


Xin Z.,Chinese Academy of Agricultural Sciences | Xin Z.,Key Laboratory of Tea Biology and Resources Utilization | Li X.,Chinese Academy of Agricultural Sciences | Li X.,Key Laboratory of Tea Biology and Resources Utilization | And 5 more authors.
BioControl | Year: 2015

Plants can respond to herbivorous attack with induced defense mechanisms. Plant elicitors play vital roles in inducing the plant defense response against herbivores. In this study, we investigated the effect of (Z)-3-hexenol (z3HOL), an important component of green leaf volatiles (GLVs), on the induced defense against a tea geometrid (TG) Ectropis obliqua Prout in tea plants. We showed that treatment with z3HOL elicites increased levels of jasmonic acid (JA) and ethylene (ET) as well as of expression of a lipoxygenase gene CsiLOX1 and a putative ACC synthase gene CsiACS1. Such reactions resulted in a marked increase in polyphenol oxidase (PPO) activity and volatile production. The induced tea plants reduced the performance of TG and became highly attractive to the main parasitoid wasp, Apanteles sp. These findings suggest that z3HOL can serve as an elicitor that triggers direct and indirect defense responses against TG by modulating signaling pathways in tea and provide a friendly strategy for biological control of pests. © 2015 International Organization for Biological Control (IOBC) Source


Wang L.,Chinese Academy of Agricultural Sciences | Wang L.,Key Laboratory of Tea Biology and Resources Utilization | Wang Y.,Chinese Academy of Agricultural Sciences | Wang Y.,Northwest Agriculture and Forestry University | And 9 more authors.
PLoS ONE | Year: 2016

Tea plant breeding is a topic of great economic importance. However, disease remains a major cause of yield and quality losses. In this study, an anthracnose-resistant cultivar, ZC108, was developed. An infection assay revealed different responses to Colletotrichum sp. infection between ZC108 and its parent cultivar LJ43. ZC108 had greater resistance than LJ43 to Colletotrichum camelliae. Additionally, ZC108 exhibited earlier sprouting in the spring, as well as different leaf shape and plant architecture. Microarray data revealed that the genes that are differentially expressed between LJ43 and ZC108 mapped to secondary metabolism-related pathways, including phenylpropanoid biosynthesis, phenylalanine metabolism, and flavonoid biosynthesis pathways. In addition, genes involved in plant hormone biosynthesis and signaling as well as plant-pathogen interaction pathways were also changed. Quantitative real-time PCR was used to examine the expression of 27 selected genes in infected and uninfected tea plant leaves. Genes encoding a MADSbox transcription factor, NBS-LRR disease-resistance protein, and phenylpropanoid metabolism pathway components (CAD, CCR, POD, beta-glucosidase, ALDH and PAL) were among those differentially expressed in ZC108. © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source


Xin Z.-J.,Chinese Academy of Agricultural Sciences | Xin Z.-J.,Key Laboratory of Tea Biology and Resources Utilization | Li X.-W.,Chinese Academy of Agricultural Sciences | Li X.-W.,Key Laboratory of Tea Biology and Resources Utilization | And 4 more authors.
Bulletin of Entomological Research | Year: 2016

Green leaf volatiles (GLVs) have been reported to play an important role in the host-locating behavior of several folivores that feed on angiosperms. However, next to nothing is known about how the green leafhopper, Empoasca vitis, chooses suitable host plants and whether it detects differing emission levels of GLV components among genetically different tea varieties. Here we found that the constitutive transcript level of the tea hydroperoxide lyase (HPL) gene CsiHPL1, and the amounts of (Z)-3-hexenyl acetate and of total GLV components are significantly higher in tea varieties that are susceptible to E. vitis (Enbiao (EB) and Banzhuyuan (BZY)) than in varieties that are resistant to E. vitis (Changxingzisun (CX) and Juyan (JY)). Moreover, the results of a Y-tube olfactometer bioassay and an oviposition preference assay suggest that (Z)-3-hexenyl acetate and (Z)-3-hexenol offer host and oviposition cues for E. vitis female adults. Taken together, the two GLV components, (Z)-3-hexenol and especially (Z)-3-hexenyl acetate, provide a plausible mechanism by which tea green leafhoppers distinguish among resistant and susceptible varieties. Future research should be carried out to obtain the threshold of the above indices and then assess their reasonableness. The development of practical detection indices would greatly improve our ability to screen and develop tea varieties that are resistant to E. vitis. Copyright © Cambridge University Press 2016 Source

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