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Xu Q.,Nanjing University of Technology | Xu Q.,Tianjin University | Li S.,Nanjing University of Technology | Huang H.,Nanjing University of Technology | And 2 more authors.
Biotechnology Advances | Year: 2012

The growing concern about the safety of food and dairy additives and the increasing costs of petroleum-based chemicals have rekindled the interest in the fermentation processes for fumaric acid production. The key problems of the industrial production of microbial fumaric acid are reviewed in this paper. Various strategies, including strain improvement, morphology control, substrate choice, fermentation process and separation process, are summarized and compared, and their economical possibilities for industrial processes are discussed. The market prospects and technological strategies for value-added fumaric acid derivatives are also addressed. The future prospects of microbial fumaric acid production are proposed at the end of this article. © 2012 Elsevier Inc. Source

Zhong C.,Tianjin University | Cao Y.-X.,Tianjin University | Li B.-Z.,Tianjin University | Yuan Y.-J.,Tianjin University | Yuan Y.-J.,Key Laboratory of Systems Bioengineering
Biofuels, Bioproducts and Biorefining | Year: 2010

Energy security and environmental stress force China to seek and develop biofuels as a substitute of fossil energy. Meanwhile, China has great potential to provide a large quantity of feedstocks for biofuel production due to its vast amount of non-food crops, such as tuberous crops, sweet sorghum, cellulosic biomass, and algae. Recently, the study and the industrial-scale production of biofuels, particularly, fuel ethanol and biodiesel, have progressed remarkably in China as a result of government preferential policies and funding supports. We have briefly reviewed the historical development of biofuels in China with special emphasis on current feedstock utilization and process technology development. The bottlenecks of utilizing various feedstocks have also been analyzed and the prospects for future biofuel development in China have been explored. Biorefineries integrating reliable, low-cost and sufficient non-food feedstock supplies with highly efficient, environmentally friendly process technologies could sustain a bright future for biofuel development in China. © 2010 Society of Chemical Industry and John Wiley & Sons, Ltd. Source

Xu S.,Tianjin University | Wang W.,CAS Tianjin Institute of Biomedical Engineering | Li X.,Tianjin Institute of Medical and Pharmaceutical Science | Liu J.,Tianjin University | And 3 more authors.
European Journal of Pharmaceutical Sciences | Year: 2014

As drug therapies become increasingly sophisticated, the synergistic benefits of two or more drugs are often required. In this study, we aimed at improving anti-tumor efficiency of paclitaxel (PTX)-incorporated thermo-sensitive injectable hydrogel by the synergy of burst release of doxorubicin hydrochloride (DOX·HCl). Thermosensitive injectable hydrogel composed of nanoparticles assembled from amphiphilic copolymer poly(ε-caprolactone-co-1,4,8-trioxa[4.6]spiro-9-undecanone)-poly(ethylene glycol)-poly(ε-caprolaone-co-1,4,8-trioxa[4.6]spiro-9-undecanone) (PECT) was fabricated. Hydrophobic PTX and hydrophilic DOX·HCl were loaded simultaneously in the thermo-sensitive injectable hydrogel by a two-stage entrapment. Thermosensitive gelling behaviors of drug-loading PECT nanoparticle aqueous dispersions were studied. In vitro release profiles of PTX and DOX·HCl and in vivo anti-tumor effect by dual drugs from PECT hydrogel were investigated. The results showed that hydrophilic and hydrophobic drugs could be successfully entrapped in PECT hydrogel simultaneously without affecting its thermo-sensitive behavior. In vitro release profiles demonstrated the burst release of DOX·HCl and the sustained release of PTX. Anti-tumor effect was improved by a fast and tense attack caused by the burst release of hydrophilic DOX·HCl from hydrogel, which was continued by the sequent sustained release of PTX-incorporated nanoparticles and remnant DOX·HCl. Unintentionally, entrapped in PECT hydrogel, hydrophilic DOX·HCl was observed to have a sustained releasing pattern in vitro and in vivo. © 2014 Elsevier B.V. All rights reserved. Source

Wang G.,Tianjin University | Huang D.,Tianjin University | Qi H.,Tianjin University | Wen J.,Tianjin University | And 4 more authors.
Bioresource Technology | Year: 2013

To rationally guide fumaric acid production improvement, metabolic profiling approach was performed to analyze metabolite changes of Rhizopus oryzae FM19 under different fermentation conditions. A correlation between the metabolic profiling and fumaric acid production was revealed by principal component analysis as well as partial least squares. Citric acid, oxaloacetic acid, 2-oxoglutarate, lactic acid, proline, alanine, valine, leucine were identified to be mainly responsible for the metabolism difference, which were involved in the Embden-Meyerhof-Parnas, tricarboxylic acid cycle, amino acid metabolism and fatty acid metabolism. Through the further analysis of metabolites changes together with the above pathways, exogenous addition strategies were developed, which resulted in 14% increase of fumaric acid (up to 56.5. g/L) and less by-products. These results demonstrated that metabolic profiling analysis could be successfully applied to the rational guidance of medium optimization and the productivity improvement of value-added compounds. © 2013 Elsevier Ltd. Source

Yu S.,Tianjin University | Huang D.,Tianjin University | Wen J.,Tianjin University | Wen J.,Key Laboratory of Systems Bioengineering | And 4 more authors.
Bioresource Technology | Year: 2012

Femtosecond laser irradiation was employed to induce mutations in Rhizopus oryzae, leading to increases in fumaric acid production. Compared to the parental strain, mutant strain FM19 exhibited an increase in titer and yield of 56.3% and 36.6%, respectively, corresponding to a titer of 49.4. g/L and a yield of 0.56. g fumaric acid per g glucose. Metabolic profiling by gas chromatography-mass spectrometry revealed that higher levels of carbon (Embden-Meyerhof-Parnas and tricarboxylic acid cycle) and amino acid metabolism were operating in the high-yielding strain; particularly, 4-aminobutyric acid and 5-aminolevulinic acid were increased 10.33- and 7.22-fold, respectively, compared with parental strain during stationary phase. These findings provided new insights into metabolic characterization of high-yielding fumaric acid R. oryzae. © 2012 Elsevier Ltd. Source

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