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Zuo T.,Chinese Academy of Fishery Sciences | Zuo T.,CAS Qingdao Institute of Oceanology | Zuo T.,Key Laboratory of Sustainable Development of Marine Fisheries | Wu Q.,Chinese Academy of Fishery Sciences | And 7 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2016

Laizhou Bay, an estuary of the Yellow River, lies to the north of the Shandong Peninsula and is one of the three largest and most important bays of the Bohai Sea. It is characterized by a high level of primary productivity and is an important spawning, nursery, and feeding ground for many fish species in the Yellow Sea and Bohai Sea. Medusae play crucial roles, not only ecologically in the marine ecosystem but also economically, as commercial target species for some large scyphomedusae in Laizhou Bay. In the past few decades, the interacting impacts of climate change and human activities have changed the ecosystem of Laizhou Bay significantly, leading to regime shifts in biological organisms, including medusae. Recently, increasing concern has prompted studies on the ecological impacts of changes in the gelatinous species community of the estuary and coastal area. To explore the temporal and spatial distribution of the composition and abundance of medusa species in the bay, annual surveys were designed and carried out from May 2011 to April 2012 (excluding December 2011 and January and February 2012, because of the presence of sea ice). Based on the data collected from 147 samples collected over the course of 9 cruises, nearly thirty cnidarians including twenty-four hydromedusae, one siphonophore, and three large scyphomedusan jellyfish were identified. The species of hydromedusae and siphonophore were small, and were collected by being vertically hauled from the seafloor to the surface using a mesoplankton net (mesh size 160 µm). Of the smaller medusae, no common species were collected throughout the year. Species richness peaked in August, while the total abundance peaked in May. One or two dominant species contributed over 80% of the total monthly abundance. The dominant species varied among months, with Rathkea octopunctata dominating from March to May, Sugiura chengshanense and Euphysora bigelowi dominating in June, E. bigelowi dominating in July, Eirene menoni dominating in September, and E. menoni and Muggiaea atlantica dominating in October. In August, E. menoni and Obelia geniculata were abundant, but were distributed regionally in the central parts of the bay. Very few medusa species were collected in November. Multiple statistical analyses, including cluster analysis and BVSTEP modules conducted with Primer 6. 0 software, were used to identify the best matches for environmental factors that affect the composition of the medusa assemblages. Based on the abundance of small medusae, cluster analysis recognized four seasonal and spatial assemblage groups. The first group, with R. octopunctata as its representative species, was collected at the runoff inlet of the bay in spring. The second group was collected in the western section of the river mouth or the central parts of the bay in summer. The second group’s representative species were S. chengshanense and E. bigelowi. The third group also occurred in summer, but its location was very similar to the spring group, and it included E. menoni as its representative species. The fourth group appeared in autumn and was collected in the river mouth or the central part of the bay, and included two indicator species: M. atlantica and Proboscidactyla flavicirrata. According to the results of the BVSTEP modules analysis, the water temperature had a more significant effect on the seasonal and spatial distribution of the hydromedusae than salinity, pH, or dissolved oxygen. The three large scyphomedusan jellyfish were only caught by fishing methods that utilized a net with a 20 mm mesh size. They occurred only at a few inlet sites after June. Aurelia spp. peaked later in October. Rhopilema esculentum and Nemopilema nomurai were collected from June to August at only a few sites. © 2016, Ecological Society of China. All rights reserved.


Liu Y.,Dalian Ocean University | Meng Y.,Dalian Ocean University | Wang Q.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.,Chinese Academy of Fishery Sciences
Molecular Biology Reports | Year: 2012

NLRs are a large family belonging to pattern recognition receptors which could recognize pathogen associated molecular patterns. Class II, major histocompatibility complex, transactivator (CIITA) is a member of NLR family. It is a critical transcription factor which could regulate the expression of MHC class II. In this study, a full-length cDNA of CIITA was cloned from channel catfish according to ten sequenced ESTs. This cDNA contains a 5′-UTR of 71 bp, a 3′-UTR of 238 bp and an ORF of 3,210 bp encoding 1,069 amino acids. Phylogenetic analysis showed that catfish CIITA was conserved with other CIITAs. Quantitative real-time PCR was conducted to detect the expression profiles of CIITA in normal tissues and responding to different pathogens (Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus (CCRV)). The expression profile in blood was the highest (53.879-fold) in normal tissues. E. tarda and S. iniae could induce catfish CIITA in head kidney, liver and spleen. CCRV virus could also induce CIITA in head kidney and liver but reduce it in spleen. And S. iniae could induce the expression of CIITA to the highest extent and contrarily CCRV virus to the lowest extent. The expression data showed the tissue-specific and pathogen-specific expression patterns of CIITA responding to different pathogens. These expression data indicated the immune-related functions of CIITA. The data obtained in this study provide a basis for further research aimed at explore the precise immune-related molecular mechanism of CIITA in catfish. © 2012 Springer Science+Business Media Dordrecht.


Yan T.,Key Laboratory of Sustainable Development of Marine Fisheries | Yan T.,Chinese Academy of Fishery Sciences | Yan T.,Shanghai Ocean University | Jiao H.,Ningbo Academy of Ocean and Fishery | And 9 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2012

Xiangshan Bay, located on the east coast of China, is a semi-enclosed bay with slow rate of water exchange. Nansha Bay, lies in the middle of Xiangshan Bay, is an important fish cage farming area. This paper presented the results of the seasonal and spatial distribution of acid volatile sulfide (AVS) in sediments from three different mariculture areas including seaweed culture area, shellfish culture area and fish cage culture area in Nansha Bay in 2007. The results revealed that AVS content in the whole investigation region ranged from 0. 01 to 30. 03 μmol/g, the average value was 3. 75 μmol/g. The mean value of AVS in shellfish culture area, seaweed culture area, fish cage culture area and the control area were 1. 03, 0. 64, 5. 06, and 0. 70 μmol/g, respectively. Although there was no significant change of AVS in surface sediment, significant difference of AVS in columnar sample (0-15cm) was found among winter, summer, spring and autumn, with the average value 3. 48, 3. 05, 8. 31 and 6. 57 μmol/g in, respectively. The value of AVS increased with the sediment depth, and reached the maximum in 6 to 9 centimeter, especially in summer and autumn. Cluster analysis showed the AVS distribution in the sediment of Nansha Bay could be divided into 3 categories: central cage area (S10) and surrounding cage area (S12 and S13); cage culture area (S6, S7 and S14) far away from central cage area; shellfish culture area (S1), algae culture area (S2) and control area (S5). The average values of AVS in different categories were 7.34, 2. 62 and 0. 75 μmol/ g, respectively. Overall, the AVS content was highest in central cage area and gradually reduced with the distance along the central cage area. The impact of fish cage farming on AVS mainly concentrated in the area within 200 m from fish cages and influence slightly far away from 500m.


Niu M.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Niu M.-X.,Shandong Provincial Key Laboratory of Fishery Resources and Ecological Environment SFREE | Niu M.-X.,Chinese Academy of Fishery Sciences | Wang J.,Key Laboratory of Sustainable Development of Marine Fisheries | And 2 more authors.
Chinese Journal of Ecology | Year: 2014

Estuary ecosystem is usually productive and can provide diverse ecosystem services, however, it is vulnerable to the effects of human activities. Effective assessment of estuary ecosystem health can provide the scientific basis for understanding the status and sustainability of the ecosystem. In this study, we reviewed theories on health assessment of estuary ecosystem, e. g. characteristics, conception and criterion, and summarized the index, technology and evaluation models as well as the rules for index choosing in the present applications. The technology of remote sensing (RS) and geographic information system (GIS) were additionally emphasized. Considering the existing difficulties in the health assessment of estuary ecosystem, we proposed a possible trend of development in this field. New technologies along with the concept, impact mechanism and spatial scale setting were suggested to be further strengthened in the future researches of estuary ecosystem health evaluation. © 2014, Editorial Board of Chinese Journal of Ecology. All rights reserved.


Niu M.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Niu M.-X.,Chinese Academy of Fishery Sciences | Niu M.-X.,Shandong Agricultural University | Wang J.,Key Laboratory of Sustainable Development of Marine Fisheries | And 7 more authors.
Chinese Journal of Ecology | Year: 2013

Based on the bottom trawl survey data in 1986-2010, and by using the techniques of GIS spatial analysis and geostatistics, this paper studied the spatiotemporal distribution and its seasonal dissimilarity of anchovy (Engraulis japonicus) stock density in Yellow Sea under the double stress of fishing pressure and climate change. There existed obvious variations in the spatial distribution of the anchovy in different seasons. In winter, the stock density of the anchovy decreased gradually from the north-eastern waters to the shore and southern waters; in spring, the stock density decreased gradually from the shore to the eastern waters; in summer, the stock density had a trend of decreased from the northern waters to the southern waters, but the high value points scattered; in autumn, similar to that in winter, the stock density decreased from the eastern waters to the shore, but the variation gradient was smaller than that in winter. The stock density center of the anchovy had an obvious seasonal variation, but the variation pattern differed with years. From 1986 to 2010, the stock density center of the anchovy moved northward in spring, summer and autumn, and had an obvious yearly transformation but no overall migration trend in winter. In different seasons, the aggregation areas of the anchovy' s high stock density differed spatially.


Sha Z.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.-X.,Chinese Academy of Fishery Sciences | Liu H.,Huazhong Agricultural University | Wang Q.-L.,Key Laboratory of Sustainable Development of Marine Fisheries | And 8 more authors.
Fish and Shellfish Immunology | Year: 2012

Protein disulfide isomerases (PDIs) are thought to aid protein folding and assembly by catalyzing formation and shuffling of cysteine disulfide bonds in the endoplasmic reticulum (ER). Currently, increasing evidence suggests PDIs play an important role in host cell invasion and they are relevant targets for the host immune response. However the roles of specific PDIs in teleosts are little known. Here, we characterized the Protein disulfide isomerase family A, member 6 (. PDIA6) from channel catfish, . Ictalurus punctatus (named as . ccPDIA6). The catfish . ccPDIA6 gene was homologous to those of other vertebrate species with 13 exons and 12 introns. The consensus full-length . ccPDIA6 cDNA contained an ORF of 1320 bp encoding a putative protein of 439 amino acids. It had a 19 amino acid signal peptide and two active thioredoxin-like domains. Sequence of phylogenic analysis and multiple alignments showed that . ccPDIA6 was conserved throughout vertebrate evolution. Southern blot analysis suggested the presence of one copy of the . ccPDIA6 gene in the catfish genome. Tissue distribution shows that . ccPDIA6 was expressed in all examined tissues at the mRNA level. When using the aquatic zoonotic pathogens such as . Edwardsiella tara, . Streptococcus iniae, and channel catfish reovirus (CCRV) to challenge channel catfish, . ccPDIA6 expression was significant changed in immune-related tissues such as head kidney, intestine, liver and spleen. The results suggested that . ccPDIA6 might play an important role in the immunity of channel catfish. This is the first report that the PDI gene may be involved in fish host defense against pathogen infection. © 2012 Elsevier Ltd.


Yang S.,Chinese Academy of Fishery Sciences | Yang S.,Key Laboratory of Sustainable Development of Marine Fisheries | Yang Q.,Chinese Academy of Fishery Sciences | Yang Q.,Key Laboratory of Sustainable Development of Marine Fisheries | And 4 more authors.
Quaternary International | Year: 2016

Contradicting findings were observed when decadal changes of primary productivity in the eastern Chinese shelf sea were learnt through examination of sediment cores (Lv, 2007; Zhou, 2007; Jin, 2009; Zhao, 2010; Yang et al., 2012). This inconformity may imply the regional differences of decadal environmental changes in different types of seas. To verify the possible regional differences, we examined sediment cores collected in different types of seas in the eastern Chinese shelf sea. 210Pb activity in the sediment was used to estimate sediment rate, while biogenic silica (BSi) was applied to indicate diatom paleoproductivity. And then the decadal change trends of diatom primary productivities (DPP) over the past 100 years in different areas were achieved. Subsequently, the differences in these DPP changes and the possible controlling mechanism were also discussed. Results reveal the presence of regional differences in DPP decadal changes in the eastern Chinese shelf sea. In all coastal seas, DPP followed similar trends before the 1980s; after the 1980s, DPP increased obviously in Shandong coastal waters where no large river mouth exists nearby, but decreased in the Yangtze River estuary. By contrast, in open waters, DPP in the past 100 years followed almost opposite trends against that in Shandong coastal waters. The differences in DPP changes between coastal and open sea areas before the 1980s could be attributed to the distinct influence mechanism of Pacific Decadal Oscillation (PDO) on DPP variation. However, the regional differences of DPP changes after the 1980s were probably attributed to the differences in influence type and intensity of human activities. Summarily, the disproportion of the influence of human activities and PDO could induce regional differences in DPP decadal changes in the Chinese shelf sea. These actual and probably general existences of regional differences prompt us to comprehensively assess long-term environmental changes in the complex shelf seas, rather than rebuild paleoenvironmental change patterns by using only one or limited sediment cores. © 2016 Elsevier Ltd and INQUA.


Zhang P.,Ocean University of China | Zhang P.,Key Laboratory of Sustainable Development of Marine Fisheries | Li C.,Ocean University of China | Li W.,Ocean University of China | And 2 more authors.
Chinese Journal of Oceanology and Limnology | Year: 2016

Accordion-shaped traps are widely used in China to catch the Asian paddle crab Charybdis japonica but traps of conventional design often catch juvenile crabs. A new type of accordion-shaped trap with an escape vent (L×W=4.3 cm×3.0 cm) was designed and a comparative study between the newly designed and conventional traps was performed in the artificial reef area of Zhuwang, Laizhou Bay, China from June to August 2012. The mean catch per unit effort (CPUE) of undersized crabs was significantly lower in the vented traps than in the conventional traps (paired t -test, n=30, P<0.001), while the CPUE of marketable crabs was significantly higher in the vented traps (paired t-test, n=30, P<0.001). The mean size of crabs (carapace length) caught in the vented traps was significantly larger than in conventional traps (paired t-test, n=29, P<0.001). The ratio of undersized crabs was 35.05±2.57% in conventional traps and 12.53±0.69% in vented traps (significantly lower, paired t-test, n=29, P<0.001). Therefore, a 4.3 cm×3.0 cm escape vent was considered appropriate for C. japonica fishing in the artificial reef area. This finding will assist the development of more sustainable and efficient crab fishing methods using accordion-shaped traps. © 2016, Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.


Niu M.,Key Laboratory of Sustainable Development of Marine Fisheries | Jin X.,Key Laboratory of Sustainable Development of Marine Fisheries | Li X.,Key Laboratory of Sustainable Development of Marine Fisheries | Wang J.,Key Laboratory of Sustainable Development of Marine Fisheries
Chinese Journal of Oceanology and Limnology | Year: 2014

We investigated the spatio-temporal and environmental factors that affect the distribution and abundance of wintering anchovy and quantifies the influences of these factors. Generalized additive models (GAMs) were developed to examine the variation in species distribution and abundance with a set of spatiotemporal and oceanographic factors, using data collected by bottom trawl surveys and remote sensing in the central and southern Yellow Sea during 2000-2011. The final model accounted for 28.21% and 41.03% of the variance in anchovy distribution and abundance, respectively. The results of a two-step GAM showed that hour, longitude, latitude, temperature gradient (TGR), and chlorophyll a (Chl-a) concentration best explained the anchovy distribution (presence/absence) and that a model including year, longitude, latitude, depth, sea surface temperature (SST), and TGR best described anchovy abundance (given presence). Longitude and latitude were the most important factors affecting both distribution and abundance, but the area of high abundance tended to be east and south of the area where anchovy were most likely to be present. Hour had a significant effect on distribution, but year was more important for anchovy abundance, indicating that the anchovy catch ratio varied across the day but abundance had an apparent interannual variation. With respect to environmental factors, TGR and Chl-a concentration had effects on distribution, while depth, SST, and TGR affected abundance. Changes in SST between two successive years or between any year and the 2000-2011 mean were not associated with changes in anchovy distribution or abundance. This finding indicated that short- and long-term water temperature changes during 2000-2011 were not of sufficient magnitude to give rise to variation in wintering anchovy distribution or abundance in the study area. The results of this study have important implications for fisheries management. © 2014 Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.


PubMed | Key Laboratory of Sustainable Development of Marine Fisheries
Type: Journal Article | Journal: Fish & shellfish immunology | Year: 2012

Protein disulfide isomerases (PDIs) are thought to aid protein folding and assembly by catalyzing formation and shuffling of cysteine disulfide bonds in the endoplasmic reticulum (ER). Currently, increasing evidence suggests PDIs play an important role in host cell invasion and they are relevant targets for the host immune response. However the roles of specific PDIs in teleosts are little known. Here, we characterized the Protein disulfide isomerase family A, member 6 (PDIA6) from channel catfish, Ictalurus punctatus (named as ccPDIA6). The catfish ccPDIA6 gene was homologous to those of other vertebrate species with 13 exons and 12 introns. The consensus full-length ccPDIA6 cDNA contained an ORF of 1320 bp encoding a putative protein of 439 amino acids. It had a 19 amino acid signal peptide and two active thioredoxin-like domains. Sequence of phylogenic analysis and multiple alignments showed that ccPDIA6 was conserved throughout vertebrate evolution. Southern blot analysis suggested the presence of one copy of the ccPDIA6 gene in the catfish genome. Tissue distribution shows that ccPDIA6 was expressed in all examined tissues at the mRNA level. When using the aquatic zoonotic pathogens such as Edwardsiella tara, Streptococcus iniae, and channel catfish reovirus CCRV to challenge channel catfish, ccPDIA6 expression was significant changed in immune-related tissues such as head kidney, intestine, liver and spleen. The results suggested that ccPDIA6 might play an important role in the immunity of channel catfish. This is the first report that the PDI gene may be involved in fish host defense against pathogen infection.

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