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Liu Y.,Dalian Ocean University | Meng Y.,Dalian Ocean University | Wang Q.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.,Chinese Academy of Fishery Sciences
Molecular Biology Reports | Year: 2012

NLRs are a large family belonging to pattern recognition receptors which could recognize pathogen associated molecular patterns. Class II, major histocompatibility complex, transactivator (CIITA) is a member of NLR family. It is a critical transcription factor which could regulate the expression of MHC class II. In this study, a full-length cDNA of CIITA was cloned from channel catfish according to ten sequenced ESTs. This cDNA contains a 5′-UTR of 71 bp, a 3′-UTR of 238 bp and an ORF of 3,210 bp encoding 1,069 amino acids. Phylogenetic analysis showed that catfish CIITA was conserved with other CIITAs. Quantitative real-time PCR was conducted to detect the expression profiles of CIITA in normal tissues and responding to different pathogens (Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus (CCRV)). The expression profile in blood was the highest (53.879-fold) in normal tissues. E. tarda and S. iniae could induce catfish CIITA in head kidney, liver and spleen. CCRV virus could also induce CIITA in head kidney and liver but reduce it in spleen. And S. iniae could induce the expression of CIITA to the highest extent and contrarily CCRV virus to the lowest extent. The expression data showed the tissue-specific and pathogen-specific expression patterns of CIITA responding to different pathogens. These expression data indicated the immune-related functions of CIITA. The data obtained in this study provide a basis for further research aimed at explore the precise immune-related molecular mechanism of CIITA in catfish. © 2012 Springer Science+Business Media Dordrecht. Source


Niu M.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Niu M.-X.,Shandong Provincial Key Laboratory of Fishery Resources and Ecological Environment SFREE | Niu M.-X.,Chinese Academy of Fishery Sciences | Wang J.,Key Laboratory of Sustainable Development of Marine Fisheries | And 2 more authors.
Chinese Journal of Ecology | Year: 2014

Estuary ecosystem is usually productive and can provide diverse ecosystem services, however, it is vulnerable to the effects of human activities. Effective assessment of estuary ecosystem health can provide the scientific basis for understanding the status and sustainability of the ecosystem. In this study, we reviewed theories on health assessment of estuary ecosystem, e. g. characteristics, conception and criterion, and summarized the index, technology and evaluation models as well as the rules for index choosing in the present applications. The technology of remote sensing (RS) and geographic information system (GIS) were additionally emphasized. Considering the existing difficulties in the health assessment of estuary ecosystem, we proposed a possible trend of development in this field. New technologies along with the concept, impact mechanism and spatial scale setting were suggested to be further strengthened in the future researches of estuary ecosystem health evaluation. © 2014, Editorial Board of Chinese Journal of Ecology. All rights reserved. Source


Sha Z.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.-X.,Chinese Academy of Fishery Sciences | Liu H.,Huazhong Agricultural University | Wang Q.-L.,Key Laboratory of Sustainable Development of Marine Fisheries | And 8 more authors.
Fish and Shellfish Immunology | Year: 2012

Protein disulfide isomerases (PDIs) are thought to aid protein folding and assembly by catalyzing formation and shuffling of cysteine disulfide bonds in the endoplasmic reticulum (ER). Currently, increasing evidence suggests PDIs play an important role in host cell invasion and they are relevant targets for the host immune response. However the roles of specific PDIs in teleosts are little known. Here, we characterized the Protein disulfide isomerase family A, member 6 (. PDIA6) from channel catfish, . Ictalurus punctatus (named as . ccPDIA6). The catfish . ccPDIA6 gene was homologous to those of other vertebrate species with 13 exons and 12 introns. The consensus full-length . ccPDIA6 cDNA contained an ORF of 1320 bp encoding a putative protein of 439 amino acids. It had a 19 amino acid signal peptide and two active thioredoxin-like domains. Sequence of phylogenic analysis and multiple alignments showed that . ccPDIA6 was conserved throughout vertebrate evolution. Southern blot analysis suggested the presence of one copy of the . ccPDIA6 gene in the catfish genome. Tissue distribution shows that . ccPDIA6 was expressed in all examined tissues at the mRNA level. When using the aquatic zoonotic pathogens such as . Edwardsiella tara, . Streptococcus iniae, and channel catfish reovirus (CCRV) to challenge channel catfish, . ccPDIA6 expression was significant changed in immune-related tissues such as head kidney, intestine, liver and spleen. The results suggested that . ccPDIA6 might play an important role in the immunity of channel catfish. This is the first report that the PDI gene may be involved in fish host defense against pathogen infection. © 2012 Elsevier Ltd. Source


Niu M.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Niu M.-X.,Chinese Academy of Fishery Sciences | Niu M.-X.,Shandong Agricultural University | Wang J.,Key Laboratory of Sustainable Development of Marine Fisheries | And 7 more authors.
Chinese Journal of Ecology | Year: 2013

Based on the bottom trawl survey data in 1986-2010, and by using the techniques of GIS spatial analysis and geostatistics, this paper studied the spatiotemporal distribution and its seasonal dissimilarity of anchovy (Engraulis japonicus) stock density in Yellow Sea under the double stress of fishing pressure and climate change. There existed obvious variations in the spatial distribution of the anchovy in different seasons. In winter, the stock density of the anchovy decreased gradually from the north-eastern waters to the shore and southern waters; in spring, the stock density decreased gradually from the shore to the eastern waters; in summer, the stock density had a trend of decreased from the northern waters to the southern waters, but the high value points scattered; in autumn, similar to that in winter, the stock density decreased from the eastern waters to the shore, but the variation gradient was smaller than that in winter. The stock density center of the anchovy had an obvious seasonal variation, but the variation pattern differed with years. From 1986 to 2010, the stock density center of the anchovy moved northward in spring, summer and autumn, and had an obvious yearly transformation but no overall migration trend in winter. In different seasons, the aggregation areas of the anchovy' s high stock density differed spatially. Source


Yan T.,Key Laboratory of Sustainable Development of Marine Fisheries | Yan T.,Chinese Academy of Fishery Sciences | Yan T.,Shanghai Ocean University | Jiao H.,Ningbo Academy of Ocean and Fishery | And 9 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2012

Xiangshan Bay, located on the east coast of China, is a semi-enclosed bay with slow rate of water exchange. Nansha Bay, lies in the middle of Xiangshan Bay, is an important fish cage farming area. This paper presented the results of the seasonal and spatial distribution of acid volatile sulfide (AVS) in sediments from three different mariculture areas including seaweed culture area, shellfish culture area and fish cage culture area in Nansha Bay in 2007. The results revealed that AVS content in the whole investigation region ranged from 0. 01 to 30. 03 μmol/g, the average value was 3. 75 μmol/g. The mean value of AVS in shellfish culture area, seaweed culture area, fish cage culture area and the control area were 1. 03, 0. 64, 5. 06, and 0. 70 μmol/g, respectively. Although there was no significant change of AVS in surface sediment, significant difference of AVS in columnar sample (0-15cm) was found among winter, summer, spring and autumn, with the average value 3. 48, 3. 05, 8. 31 and 6. 57 μmol/g in, respectively. The value of AVS increased with the sediment depth, and reached the maximum in 6 to 9 centimeter, especially in summer and autumn. Cluster analysis showed the AVS distribution in the sediment of Nansha Bay could be divided into 3 categories: central cage area (S10) and surrounding cage area (S12 and S13); cage culture area (S6, S7 and S14) far away from central cage area; shellfish culture area (S1), algae culture area (S2) and control area (S5). The average values of AVS in different categories were 7.34, 2. 62 and 0. 75 μmol/ g, respectively. Overall, the AVS content was highest in central cage area and gradually reduced with the distance along the central cage area. The impact of fish cage farming on AVS mainly concentrated in the area within 200 m from fish cages and influence slightly far away from 500m. Source

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