Key Laboratory of Sustainable Development of Marine Fisheries

Key Laboratory of Sustainable Development of Marine Fisheries

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Lv J.,Key Laboratory of Sustainable Development of Marine Fisheries | Lv J.,Chinese Academy of Fishery Sciences | Lv J.,Laboratory for Marine Fisheries and Aquaculture | Zhang L.,Key Laboratory of Sustainable Development of Marine Fisheries | And 8 more authors.
PLoS ONE | Year: 2017

Molting is an essential biological process throughout the life history of crustaceans, which is regulated by many neuropeptide hormones expressed in the eyestalk. To better understand the molting mechanism in Portunus trituberculatus, we used digital gene expression (DGE) to analyze single eyestalk samples during the molting cycle by high-throughput sequencing. Results We obtained 14,387,942, 12,631,508 and 13,060,062 clean sequence reads from inter-molt (InM), pre-molt (PrM) and post-molt (PoM) cDNA libraries, respectively. A total of 1,394 molt-related differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analysis identified some important processes and pathways with key roles in molting regulation, such as chitin metabolism, peptidase inhibitor activity, and the ribosome. We first observed a pattern associated with the neuromodulator-related pathways during the molting cycle, which were up-regulated in PrM and down-regulated in PoM. Four categories of important molting-related transcripts were clustered and most of them had similar expression patterns, which suggests that there is a connection between these genes throughout the molt cycle. Conclusion Our work is the first molt-related investigation of P. trituberculatus focusing on the eyestalk at the whole transcriptome level. Together, our results, including DEGs, identification of molting-related biological processes and pathways, and observed expression patterns of important genes, provide a novel insight into the function of the eyestalk in molting regulation. © 2017 Lv et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Niu M.X.,Chinese Academy of Fishery Sciences | Niu M.X.,Key Laboratory of Sustainable Development of Marine Fisheries | Niu M.X.,Shandong Provincial Key Laboratory of Fishery Resources and Ecological Environment SFREE | Wang J.,Chinese Academy of Fishery Sciences | And 4 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2017

As the main channel of matter exchange between riverine and marine environments, estuarine ecosystems have been subjected to growing environmental interference and disturbance. Anthropogenic activities and the interactions between land and sea considerably affect estuarine ecosystems and health, and they have resulted in challenges for ecosystem management. Ecosystem health assessment, a new method of ecosystem assessment, determines the current ecosystem structure and function, and provides substantial information necessary for effective ecosystem management. Therefore, for the management of the Yellow River Estuary, a pressure-state-response (PSR) method was used to assess the health status of the ecosystem. The estuary and the adjacent marine and terrestrial ecosystems were considered an entity under the broad definition of estuarine ecosystems. An ecosystem health assessment indicator system was developed, taking externalpressure, ecosystem state, and response parameters into consideration to calculate indicators of regional ecosystem health. The health status of the Yellow River estuarine ecosystem was then assessed using a comprehensive evaluation index (CEI), including historical data from 1991. The results showed that the overall index of the Yellow River Estuary was 0.7427 in October 2013. In detail, the response index was 0.9055, which indicates that the Yellow River estuarine ecosystem was “very healthy,” suggesting the policy and behavior of local government, relevant departments, and individuals reduced the pressure on the regional environment to a certain extent. The pressure index was 0.8288, which indicates that the ecosystem was at a “good status,” but has been under growing pressure and its health has deteriorated. The state index was 0.6458, suggesting that the ecosystem is “unhealthy” and is under considerable pressure, requiring improvement. Overall, the current status of the Yellow River estuarine ecosystem was reasonably “healthy” as compared to that in 1991, but it has deteriorated. The state index showed the most serious decline among pressure, state, and response indexes. The main reasons for the health degradation of the Yellow River estuarine ecosystem were investigated by analyzing the index variation and its weight. The factors resulting in the deterioration included overfishing, unreasonable wetland development, over- farming in shallow seawater, and pollution. Therefore, actions should be taken to prevent further deterioration through ecological restoration and appropriate management of the Yellow River estuarine ecosystem. Any future development should be based on scientific planning for the sustainability of the ecosystem. © 2017, Ecological Society of China. All rights reserved.


Tian Y.,Key Laboratory of Sustainable Development of Marine Fisheries | Duan H.,Key Laboratory of Sustainable Development of Marine Fisheries | Li X.,Key Laboratory of Sustainable Development of Marine Fisheries | Li H.,Key Laboratory of Sustainable Development of Marine Fisheries | And 5 more authors.
Journal of Fishery Sciences of China | Year: 2017

Establishing meiotic gynogenesis, mitotic gynogenesis, and inbred lines is important for trait purification during selective fish breeding. Yet how these three genetic pathways affect heredity remains the focus of much genetics research. In this study, we used the female parents from Paralichthys olivaceus family F09119 to establish mitotic gynogenesis (F1346), meiotic gynogenesis (F1324), and an inbred line (F1313) and analyzed their growth and genetic characteristics. Body length, body width, and weight were measured in the three families from 85 to 388 d post fertilization (dpf). Results on 338 dpf showed that growth rate of F1346 was the highest [body length, (28.89±2.77) cm; body width, (10.00±1.21) cm; and weight, (254.91±83.11) g] and that of F1324 was the lowest (P < 0.05). Survival rate of F1324 on 263 dpf was the highest (46.50%) and that of F1346 was the lowest (26.80%). The numbers of alleles for mitotic gynogenesis, meiotic gynogenesis, and the inbred line were 2.0, 2.0 and 2.35, respectively. The effective numbers of alleles were 1.87, 1.95, and 2.1; polymorphic information content values were 0.35, 0.36, and 0.4; and mean observed heterozygosity values were 0, 0.8155, and 0.6366, respectively, indicating that genetic variation of mitotic gynogenesis and meiotic gynogenesis was less than that of the inbred line. The trend for unbiased expected homozygosity was F1313 (0.503) < F1324 (0.5105) < F1346 (0.532) and that of unbiased expected heterozygosity was F1313 (0.4967) > F1324 (0.4894) > F1346 (0.4679). Homozygosity of meiotic gynogenesis was 1.0421-fold higher than that of meiotic gynogenesis and 1.0577-fold higher than that of the inbred line, whereas that of meiotic gynogenesis was 1.0149-fold higher than that of the inbred line. The genetic distance between F1346 and F1313 was longest (0.1568) but genetic similarity was the lowest (0.8549), whereas the genetic distance between F1346 and F1324 was the shortest (0.0406), and genetic similarity was the greatest (0.9602). These results provide a theoretical basis for establishing an inbred line of P. olivaceus and analyses of genetic variations among meiotic gynogenesis, mitotic gynogenesis, and the inbred line.


Liu Y.,Dalian Ocean University | Meng Y.,Dalian Ocean University | Wang Q.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.,Chinese Academy of Fishery Sciences
Molecular Biology Reports | Year: 2012

NLRs are a large family belonging to pattern recognition receptors which could recognize pathogen associated molecular patterns. Class II, major histocompatibility complex, transactivator (CIITA) is a member of NLR family. It is a critical transcription factor which could regulate the expression of MHC class II. In this study, a full-length cDNA of CIITA was cloned from channel catfish according to ten sequenced ESTs. This cDNA contains a 5′-UTR of 71 bp, a 3′-UTR of 238 bp and an ORF of 3,210 bp encoding 1,069 amino acids. Phylogenetic analysis showed that catfish CIITA was conserved with other CIITAs. Quantitative real-time PCR was conducted to detect the expression profiles of CIITA in normal tissues and responding to different pathogens (Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus (CCRV)). The expression profile in blood was the highest (53.879-fold) in normal tissues. E. tarda and S. iniae could induce catfish CIITA in head kidney, liver and spleen. CCRV virus could also induce CIITA in head kidney and liver but reduce it in spleen. And S. iniae could induce the expression of CIITA to the highest extent and contrarily CCRV virus to the lowest extent. The expression data showed the tissue-specific and pathogen-specific expression patterns of CIITA responding to different pathogens. These expression data indicated the immune-related functions of CIITA. The data obtained in this study provide a basis for further research aimed at explore the precise immune-related molecular mechanism of CIITA in catfish. © 2012 Springer Science+Business Media Dordrecht.


Niu M.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Niu M.-X.,Shandong Provincial Key Laboratory of Fishery Resources and Ecological Environment SFREE | Niu M.-X.,Chinese Academy of Fishery Sciences | Wang J.,Key Laboratory of Sustainable Development of Marine Fisheries | And 2 more authors.
Chinese Journal of Ecology | Year: 2014

Estuary ecosystem is usually productive and can provide diverse ecosystem services, however, it is vulnerable to the effects of human activities. Effective assessment of estuary ecosystem health can provide the scientific basis for understanding the status and sustainability of the ecosystem. In this study, we reviewed theories on health assessment of estuary ecosystem, e. g. characteristics, conception and criterion, and summarized the index, technology and evaluation models as well as the rules for index choosing in the present applications. The technology of remote sensing (RS) and geographic information system (GIS) were additionally emphasized. Considering the existing difficulties in the health assessment of estuary ecosystem, we proposed a possible trend of development in this field. New technologies along with the concept, impact mechanism and spatial scale setting were suggested to be further strengthened in the future researches of estuary ecosystem health evaluation. © 2014, Editorial Board of Chinese Journal of Ecology. All rights reserved.


Sha Z.-X.,Key Laboratory of Sustainable Development of Marine Fisheries | Sha Z.-X.,Chinese Academy of Fishery Sciences | Liu H.,Huazhong Agricultural University | Wang Q.-L.,Key Laboratory of Sustainable Development of Marine Fisheries | And 8 more authors.
Fish and Shellfish Immunology | Year: 2012

Protein disulfide isomerases (PDIs) are thought to aid protein folding and assembly by catalyzing formation and shuffling of cysteine disulfide bonds in the endoplasmic reticulum (ER). Currently, increasing evidence suggests PDIs play an important role in host cell invasion and they are relevant targets for the host immune response. However the roles of specific PDIs in teleosts are little known. Here, we characterized the Protein disulfide isomerase family A, member 6 (. PDIA6) from channel catfish, . Ictalurus punctatus (named as . ccPDIA6). The catfish . ccPDIA6 gene was homologous to those of other vertebrate species with 13 exons and 12 introns. The consensus full-length . ccPDIA6 cDNA contained an ORF of 1320 bp encoding a putative protein of 439 amino acids. It had a 19 amino acid signal peptide and two active thioredoxin-like domains. Sequence of phylogenic analysis and multiple alignments showed that . ccPDIA6 was conserved throughout vertebrate evolution. Southern blot analysis suggested the presence of one copy of the . ccPDIA6 gene in the catfish genome. Tissue distribution shows that . ccPDIA6 was expressed in all examined tissues at the mRNA level. When using the aquatic zoonotic pathogens such as . Edwardsiella tara, . Streptococcus iniae, and channel catfish reovirus (CCRV) to challenge channel catfish, . ccPDIA6 expression was significant changed in immune-related tissues such as head kidney, intestine, liver and spleen. The results suggested that . ccPDIA6 might play an important role in the immunity of channel catfish. This is the first report that the PDI gene may be involved in fish host defense against pathogen infection. © 2012 Elsevier Ltd.


Yang S.,Chinese Academy of Fishery Sciences | Yang S.,Key Laboratory of Sustainable Development of Marine Fisheries | Yang Q.,Chinese Academy of Fishery Sciences | Yang Q.,Key Laboratory of Sustainable Development of Marine Fisheries | And 4 more authors.
Quaternary International | Year: 2016

Contradicting findings were observed when decadal changes of primary productivity in the eastern Chinese shelf sea were learnt through examination of sediment cores (Lv, 2007; Zhou, 2007; Jin, 2009; Zhao, 2010; Yang et al., 2012). This inconformity may imply the regional differences of decadal environmental changes in different types of seas. To verify the possible regional differences, we examined sediment cores collected in different types of seas in the eastern Chinese shelf sea. 210Pb activity in the sediment was used to estimate sediment rate, while biogenic silica (BSi) was applied to indicate diatom paleoproductivity. And then the decadal change trends of diatom primary productivities (DPP) over the past 100 years in different areas were achieved. Subsequently, the differences in these DPP changes and the possible controlling mechanism were also discussed. Results reveal the presence of regional differences in DPP decadal changes in the eastern Chinese shelf sea. In all coastal seas, DPP followed similar trends before the 1980s; after the 1980s, DPP increased obviously in Shandong coastal waters where no large river mouth exists nearby, but decreased in the Yangtze River estuary. By contrast, in open waters, DPP in the past 100 years followed almost opposite trends against that in Shandong coastal waters. The differences in DPP changes between coastal and open sea areas before the 1980s could be attributed to the distinct influence mechanism of Pacific Decadal Oscillation (PDO) on DPP variation. However, the regional differences of DPP changes after the 1980s were probably attributed to the differences in influence type and intensity of human activities. Summarily, the disproportion of the influence of human activities and PDO could induce regional differences in DPP decadal changes in the Chinese shelf sea. These actual and probably general existences of regional differences prompt us to comprehensively assess long-term environmental changes in the complex shelf seas, rather than rebuild paleoenvironmental change patterns by using only one or limited sediment cores. © 2016 Elsevier Ltd and INQUA.


Zhang P.,Ocean University of China | Zhang P.,Key Laboratory of Sustainable Development of Marine Fisheries | Li C.,Ocean University of China | Li W.,Ocean University of China | And 2 more authors.
Chinese Journal of Oceanology and Limnology | Year: 2016

Accordion-shaped traps are widely used in China to catch the Asian paddle crab Charybdis japonica but traps of conventional design often catch juvenile crabs. A new type of accordion-shaped trap with an escape vent (L×W=4.3 cm×3.0 cm) was designed and a comparative study between the newly designed and conventional traps was performed in the artificial reef area of Zhuwang, Laizhou Bay, China from June to August 2012. The mean catch per unit effort (CPUE) of undersized crabs was significantly lower in the vented traps than in the conventional traps (paired t -test, n=30, P<0.001), while the CPUE of marketable crabs was significantly higher in the vented traps (paired t-test, n=30, P<0.001). The mean size of crabs (carapace length) caught in the vented traps was significantly larger than in conventional traps (paired t-test, n=29, P<0.001). The ratio of undersized crabs was 35.05±2.57% in conventional traps and 12.53±0.69% in vented traps (significantly lower, paired t-test, n=29, P<0.001). Therefore, a 4.3 cm×3.0 cm escape vent was considered appropriate for C. japonica fishing in the artificial reef area. This finding will assist the development of more sustainable and efficient crab fishing methods using accordion-shaped traps. © 2016, Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.


Niu M.,Key Laboratory of Sustainable Development of Marine Fisheries | Jin X.,Key Laboratory of Sustainable Development of Marine Fisheries | Li X.,Key Laboratory of Sustainable Development of Marine Fisheries | Wang J.,Key Laboratory of Sustainable Development of Marine Fisheries
Chinese Journal of Oceanology and Limnology | Year: 2014

We investigated the spatio-temporal and environmental factors that affect the distribution and abundance of wintering anchovy and quantifies the influences of these factors. Generalized additive models (GAMs) were developed to examine the variation in species distribution and abundance with a set of spatiotemporal and oceanographic factors, using data collected by bottom trawl surveys and remote sensing in the central and southern Yellow Sea during 2000-2011. The final model accounted for 28.21% and 41.03% of the variance in anchovy distribution and abundance, respectively. The results of a two-step GAM showed that hour, longitude, latitude, temperature gradient (TGR), and chlorophyll a (Chl-a) concentration best explained the anchovy distribution (presence/absence) and that a model including year, longitude, latitude, depth, sea surface temperature (SST), and TGR best described anchovy abundance (given presence). Longitude and latitude were the most important factors affecting both distribution and abundance, but the area of high abundance tended to be east and south of the area where anchovy were most likely to be present. Hour had a significant effect on distribution, but year was more important for anchovy abundance, indicating that the anchovy catch ratio varied across the day but abundance had an apparent interannual variation. With respect to environmental factors, TGR and Chl-a concentration had effects on distribution, while depth, SST, and TGR affected abundance. Changes in SST between two successive years or between any year and the 2000-2011 mean were not associated with changes in anchovy distribution or abundance. This finding indicated that short- and long-term water temperature changes during 2000-2011 were not of sufficient magnitude to give rise to variation in wintering anchovy distribution or abundance in the study area. The results of this study have important implications for fisheries management. © 2014 Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.


PubMed | Key Laboratory of Sustainable Development of Marine Fisheries
Type: Journal Article | Journal: Fish & shellfish immunology | Year: 2012

Protein disulfide isomerases (PDIs) are thought to aid protein folding and assembly by catalyzing formation and shuffling of cysteine disulfide bonds in the endoplasmic reticulum (ER). Currently, increasing evidence suggests PDIs play an important role in host cell invasion and they are relevant targets for the host immune response. However the roles of specific PDIs in teleosts are little known. Here, we characterized the Protein disulfide isomerase family A, member 6 (PDIA6) from channel catfish, Ictalurus punctatus (named as ccPDIA6). The catfish ccPDIA6 gene was homologous to those of other vertebrate species with 13 exons and 12 introns. The consensus full-length ccPDIA6 cDNA contained an ORF of 1320 bp encoding a putative protein of 439 amino acids. It had a 19 amino acid signal peptide and two active thioredoxin-like domains. Sequence of phylogenic analysis and multiple alignments showed that ccPDIA6 was conserved throughout vertebrate evolution. Southern blot analysis suggested the presence of one copy of the ccPDIA6 gene in the catfish genome. Tissue distribution shows that ccPDIA6 was expressed in all examined tissues at the mRNA level. When using the aquatic zoonotic pathogens such as Edwardsiella tara, Streptococcus iniae, and channel catfish reovirus CCRV to challenge channel catfish, ccPDIA6 expression was significant changed in immune-related tissues such as head kidney, intestine, liver and spleen. The results suggested that ccPDIA6 might play an important role in the immunity of channel catfish. This is the first report that the PDI gene may be involved in fish host defense against pathogen infection.

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