Ye B.,Fujian Normal University |
Ye B.,Key Laboratory of Sugarcane Genetics and Breeding |
Qiu S.,Fujian Normal University |
Qiu S.,Key Laboratory of Sugarcane Genetics and Breeding |
And 5 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2011
To achieve the ultimate aim of increasing sucrose content in the sugarcane stem, it is necessary to investigate the principal rate-limiting steps in the pathway of sucrose synthesis. It has been suggested that sucrose phosphate synthase (SPS) is a key rate-limiting enzyme in sucrose formation and it also influences sucrose accumulation and carbon partitioning processes. Relative expression of SPS II gene occupies 40% of the total transcription of SPS gene, which shows that the cloning and expression of SPSU gene influences sucrose accumulation process. This research focused on molecular cloning and expression analysis of SPSII gene in sugarcane. The SPSII cDNA fragment was isolated from sugarcane, which contained a 3 183 bp open reading frame (ORF), encoding 1 060 amino acids. Its Genbank accession no. EU 269038. Relative expression of SPSII gene was determined in the different tissues of sugarcane at different time of sugar accumulation by real-time PCR. The results showed highest relative expression of SPSII gene in the canes at early stage during accumulation process of sucrose, but highest in the leaves at middle stage. In the same photosynthetic tissue, relative expression of SPSII was higher at early stage during accumulation process of sucrose than those at middle stage and later stage.