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Shijiazhuang, China

Dong Y.,Hebei Medical University | Wang J.,Hebei Medical University | Wang J.,Key Laboratory of Stomatology | Dong F.,Hebei Medical University | And 2 more authors.
Journal of Oral Pathology and Medicine

Objective: To evaluate relationships between the alteration of p16 gene and the clinical status and prognosis of the patients with squamous cell carcinoma of the buccal mucosa. Methods: Thirty buccal cancers were included in the analysis. Deletion analysis was performed by PCR. Point mutation analysis was used by PCR-SSCP and direct sequencing. Methylation-specific PCR methods were adopted for the evaluation of p16 methylation. The correlation between alteration of p16 gene and clinicopathological factors buccal cancer was evaluated by Fisher's exact test. Kaplan-Meier and Cox regression were used to investigate the relationship between p16 alteration and survival time. Results: The frequency of p16 alteration was 63.3% in buccal carcinomas. P16 deletion was associated significantly with tumor size (P=0.01). P16 point mutation was associated significantly with differentiation (P=0.006). P16 methylation was associated significantly with nodes metastasis (P=0.027). The overall survival rate of 30 buccal carcinomas was 53.3%. The Log-rank test (P= 0.021) and univariate Cox regression analysis (P=0.030) revealed that p16 methylation was significantly associated with the overall survival rate. Multivariate analysis showed that p16 deletion, p16 mutation, and p16 methylation were not statistically significant. Conclusions: The alterations of p16 gene may play a major role in malignancy and development and metastases of buccal carcinoma and may be an excellent marker of aggressive clinical behavior. P16 methylation has a prognostic value in buccal carcinoma but not an independent prognosis factor. P16 point mutation and p16 deletion have not prognostic significance in buccal carcinoma. © 2012 John Wiley & Sons A/S. Source

Dong Y.,Hebei Medical University | Dong F.,Hebei Medical University | Zhang X.,Key Laboratory of Stomatology | Hao F.,Hebei Medical University | And 4 more authors.
International Journal of Oral and Maxillofacial Surgery

The aim of this study was to compare velopharyngeal closure between patients who underwent Furlow palatoplasty and two-flap palatoplasty. A retrospective review of 88 patients with incomplete palate cleft was performed. 48 patients (17 males; 31 females) aged 2-28 years received Furlow palatoplasty. 40 patients (17 males; 23 females) aged 2-21 years received two-flap palatoplasty. Velopharyngeal function was categorized as adequate, marginal or inadequate. Complications associated with the operation were documented. Statistically significant differences were not found amongst sex distribution, age at operation, follow-up time, and preoperative speech intelligibility. After primary repairs using Furlow and two-flap palatoplasty, the surgeon's incidence of postoperative palatal fistula was 0%. The complications were not significantly different between the two groups. The authors achieved the lowest reported incidence of postoperative palatal fistulas in primary Furlow palatoplasty. The outcomes of the velopharyngeal closure were better in patients who received Furlow palatoplasty (P < 0.05). Furlow palatoplasty was more effective than two-flap palatoplasty in obtaining perfect velopharyngeal closure. A probable explanation may be that Furlow palatoplasty can reposition and overlap the divergent palatal muscle and lengthen the soft palate. © 2012 International Association of Oral and Maxillofacial Surgeonss. Published by Elsevier Ltd. All rights reserved. Source

Shi J.,Shanghai JiaoTong University | Shi J.,Key Laboratory of Stomatology | Jiao F.,Shanghai Stomatological Disease Center | Xu B.,Shanghai JiaoTong University | And 3 more authors.
Journal of Craniofacial Surgery

Purpose: Open reduction and internal fixation of intracapsular condylar fractures is a great challenge due to the confined access. This study aims to explore the feasibility of transparotid approach in the treatmentof intracapsular condylar fractures. Patients and Methods: Eight patients with intracapsular condylar fractures were enrolled in this study. A minimized preauricular incision and transparotid access was used. Blunt dissection was performed perpendicularly to the condyle. The reduction and fixation of intracapsular condylar fractureswere performed. After confirming that the fracture was fixed rigidly, the articular disc was repositioned.Results: Using this incision, the condylar head and fracture stump were exposed perpendicularly. No extensive incision was needed and minimal invasion was realized. Postoperative CT scan showed that the condyle had been repositioned and fixed in the normal position. Occlusal disturbance, restriction of mouth opening, and lateraldeviation were not found in the follow-up. Conclusions: Transparotid approach gave an optimal viewof the bony field, which allowed surgeons to work perpendicularly to the fracture, and facilitated the reduction of medially displaced proximal stumps. It was regarded as an ideal and valuable alternative in this potentially complicated procedure. © 2015 Mutaz B. Habal, MD. Source

Zhang Y.,Hebei Medical University | Zhang Y.,Key Laboratory of Stomatology | Wang J.,Hebei Medical University | Wang J.,Key Laboratory of Stomatology | And 6 more authors.
Archives of Oral Biology

Objective Proteoglycans play a crucial role in salivary adenoid cystic carcinoma (SACC) tumorigenesis, neurotropic growth and lung metastasis. In this study, we investigated the role of GPC5 in the lung metastasis of SACC. Design The expression of heparan sulfate proteoglycans (HSPGs) was detected in SACC-M (high lung-metastatic cell line), SACC-2 (low lung-metastatic cell line) and SACC-83 (low lung-metastatic cell line) cells by relative quantitative Real-Time PCR. The expression of GPC5 was detected by immunofluorescence and Western blot analysis in SACC-M, SACC-2 and SACC-83 cells, and by immunohistochemical analysis in primary tumours from 16 cases of SACC patients with or without lung metastasis. GPC5 expression was silenced in SACC-M cells, cell proliferation and tumour growth was evaluated by MTT assay and nude mice model, respectively. Results Expression of most HSPGs was decreased in SACC-M cells, but GPC5 expression increased 3.24-fold and 815.69-fold (more than 3-fold) in SACC-M compared with SACC-2 and SACC-83 cells, respectively. Immunohistochemical analysis showed higher expression of GPC5 in SACC with lung metastasis compared to SACC without lung metastasis. The lung metastasis of SACC-M cells in nude mice was obviously decreased after GPC5 silencing (P < 0.05), although GPC5 silencing had no significant effect on SACC-M cell proliferation. Conclusion GPC5 may contribute to lung metastasis of SACC. © 2014 Elsevier Ltd. Source

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