Yang T.,Key Laboratory of Standardization of Chinese Medicines |
Yang T.,Shanghai University of Traditional Chinese Medicine |
Wang C.,Key Laboratory of Standardization of Chinese Medicines |
Wang C.,Shanghai University of Traditional Chinese Medicine |
And 12 more authors.
Pharmaceutical Biology | Year: 2010
An activity-directed fractionation and purification process was used to isolate 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging components from fruits of Capparis spinosa L. (Capparaeae). Ethyl acetate and aqueous fractions showed greater DPPH• scavenging activities compared to the petroleum ether fractions. The ethyl acetate fraction was subjected to purification using column chromatography. A new antioxidant cappariside (4-hydroxy-5-methylfuran-3-carboxylic acid, 1), together with seven known organic acids (28) for the first time from plants of genus Capparis and four known organic acids (912) were isolated from C. spinosa. The structures were elucidated by extensive analysis of 1D- and 2D-NMR spectroscopic. In addition, compounds 1, 2, 4, 5, 9, 10 and 12 indicated strong scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals with a SC50 value of 0.204±0.002, 0.007±0.0, 0.011±0.0, 0.044±0.0016, 0.032±0.0, 0.090±0.001, and 0.350±0.017mM, respectively. © 2010 Informa UK Ltd.