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Zha D.-M.,Chinese Academy of Agricultural Sciences | Zha D.-M.,Jiangsu University of Science and Technology | Zha D.-M.,State Key Laboratory of Special Economic Animal Molecular Biology | Zha D.-M.,Key Laboratory of Special Economic Animal Germplasm Resources and Genetic Improvement | And 6 more authors.
Food Chemistry | Year: 2011

Attempts were made to establish one-step multiplex PCR assay for the identification of the widely used species in deer products (sika deer, wapiti, red deer and reindeer). Primers were designed from tandem repeat region of D-loop and well-conserved region of 16S rDNA after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 307 bp in length for sika deer, 307 and 246 bp for wapiti, 272 bp for Tarim red deer, 230 bp for red deer and 141 bp for reindeer, respectively. The detection limit was 0.05 ng for sika deer and wapiti, 0.1 ng for Tarim red deer, 0.5 ng for red deer and 0.02 ng for reindeer. The results demonstrated that the fraudulent phenomenon is epidemic in the substitution of deer products, in especially antler, penis, foetus and tendon products. Hence, this multiplex PCR provided a useful and sensitive technique to identify the sources of deer products. © 2011 Elsevier Ltd. All rights reserved.


Zha D.,Chinese Academy of Agricultural Sciences | Zha D.,Jiangsu University of Science and Technology | Zha D.,State Key Laboratory of Special Economic Animal Molecular Biology | Zha D.,Key Laboratory of Special Economic Animal Germplasm Resources and Genetic Improvement | And 6 more authors.
Food Control | Year: 2010

Attempts were made to established one-step multiplex PCR assay for the fraud identification of the mostly used species in deer products (bovine, ovine, porcine and poultry). Primers were selected from published papers or designed in the well-conserved region of tRNA-Val and 16S rRNA mitochondrial genes after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 124, 183, 225 and 290. bp length for bovine, poultry, ovine and porcine, respectively. The detection limit was 1. ng for porcine and ovine primers, 5. ng for poultry primers and 0.5. ng for bovine primers. The results demonstrated that fraud phenomena are very epidemic in the deer products, especially heart, blood, penis and antler products. The multiplex PCR described in this study, proved to be very sensitive and reliable in species identification, could be considered as a further improvement of traditional methods based assay for the identification of deer products. © 2010 Elsevier Ltd.

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