Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation

Kunming, China

Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation

Kunming, China
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Chen W.-M.,Yunnan Academy of Agricultural Sciences | Chen W.-M.,Yunnan Provincial Key Laboratory of Agricultural Biotechnology | Chen W.-M.,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation | Chai H.-M.,Yunnan Academy of Agricultural Sciences | And 14 more authors.
Annals of Microbiology | Year: 2012

The diversity of Agrocybe aegerita multispecies complex in Yunnan Province, southwest China was investigated. In total, 23 strains collected from ten districts were classified into three groups through mitochondrial smallsubunit (mtSSU) rDNA sequence analysis. Phylogeny of internal transcribed spacer (ITS) combined with mating tests demonstrated that the tested strains included at least two species. Agrocybe salicacola, which is difficult to distinguish from other species in the A. aegerita complex, was first systematically analyzed and identified with molecular characterization. YAASM0594 and YAASM0625 share sequences with YAASM1024, and XW01 in group B, defined according to the mtSSU rDNA sequences, was related more closely to group C in the ITS phylogenetic tree, indicating that group B and CS45 are closely related, with both groups possibly even being the same species. We conclude that a high diversity occurs in Agrocybe aegerita complex than reported previously, and that more novel species would possibly be discovered through molecular phylogeny and mating tests. © 2012 Springer-Verlag and the University of Milan.


Chen W.,Yunnan Academy of Agricultural Sciences | Chen W.,Yunnan Provincial Key Laboratory of Agricultural Biotechnology | Chen W.,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation | Chai H.,Yunnan Academy of Agricultural Sciences | And 11 more authors.
Current Microbiology | Year: 2017

Agrocybe salicacola is a delicious and cultivable mushroom. It is important to understand this species’ inherent characteristics, especially to elucidate the constitution and segregation of mating genes. In this study, two compatible B mating loci in strain YAASM0711 of A. salicacola were cloned from the monokaryons, and sequence and phylogeny analyses showed two conserved genes encoding pheromone receptors maybe lost mating activity, which determined by comparing with those of other mushrooms. In the conserved regions of mating loci, partial insertion/deletion fragments made non-coding regions posses polymorphisms, and monokaryotic strains of different mating types were distinguished from each other according to the amplification profile of variable regions, which suggested mating loci were integrally assigned to offspring strains during mitosis in A. salicacola. As our known, it is the first to develop molecular markers for B mating-type identification using variable non-coding fragments of mating loci in basidiomycetes. © 2017, Springer Science+Business Media New York.


Zheng K.,Yunnan Academy of Agricultural Sciences | Zheng K.,Yunnan Provincial Key Laboratory of Agricultural Biotechnology | Zheng K.,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation | Liu H.,HongyunHonghe Group Ltd Company | And 12 more authors.
Archives of Virology | Year: 2016

The virus isolate 2009-GZT, collected from tomato in Guizhou province of China, was identified as a new member of the genus Tospovirus based on its S RNA sequence. Because its provisional name, “tomato necrotic spot virus” (TNSV), was identical to an already existing member of the genus Ilarvirus, 2009-GZT was renamed “tomato necrotic spot-associated virus” (TNSaV). In this study, the full-length sequences of the genomic M and L RNAs of TNSaV were determined and analyzed. The M RNA has 4,773 nucleotides (nt), encoding the NSm protein of 309 aa (34.4 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1123 aa (128 kDa) in the viral complementary (vc) strand. The NSm and Gn/Gc of TNSaV share the highest aa sequence identity (86.2 % and 86.9 %, respectively) with those of tomato zonate spot virus. The L RNA contains 8,908 nt and codes for the putative RNA-dependent RNA polymerase (RdRp) of 2885 aa (332 kDa) in the vc strand. The RdRp of TNSaV shares the highest aa sequence identity (85.2 %) with that of calla lily chlorotic spot virus (CCSV). Serological assays showed that TNSaV cross-reacts with rabbit antisera against watermelon silver mottle virus (WSMoV) NP and CCSV NP, indicating that TNSaV is a member of the WSMoV serogroup. © 2016, Springer-Verlag Wien.


Chen L.-J.,Yunnan University | Chen L.-J.,Yunnan Academy of Agricultural Sciences | Chai H.-M.,Yunnan Academy of Agricultural Sciences | Chai H.-M.,Yunnan Provincial Key Laboratory of Agricultural Biotechnology | And 10 more authors.
Indian Journal of Microbiology | Year: 2014

The difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium-calcium exchange protein and keratin-associated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some were only putative gene fragments. All these fragments were speculated to be the functional gene associated with sclerotial formation in morel. © 2014 Association of Microbiologists of India.


Chen W.,Yunnan Academy of Agricultural Sciences | Chen W.,Yunnan Provincial Key Laboratory of Agricultural Biotechnology | Chen W.,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation | Zhang X.,Yunnan Academy of Agricultural Sciences | And 17 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2016

The rare introns with GC-AG border are found in some organisms including human, Caenorhabditis elegans and filamentous fungi with low detection rate of 0.6%-1.2%. In general, this type of introns seem to be related to the alternative splice, and their retention or removal regulate the function of the translated protein. It is necessary to obtain the glycerol-3-phosphate dehydrogenase (GPD) encoding gene from Agrocybe salicacola strain M20 and to understand its splicing characterization. This study cloned the complete gpd gene containing GC-AG intron from single spore isolation M20 of Agrocybe salicacola YAASM0711 by using PCR with genomic sequencing data. The population investigation showed two types of GC-AG introns amplified by special primers, both found in each of the two species A. salicacola and A. aegerita, which indicated that they probably occurred before the species divergence. In addition, alternative splice was not detected in the mycelia of M20 or any development stage of YAASM0711. Gene expression showed the highest mRNA level of gpd existing in young fruiting body, and probably playing an important role during the rapid growth of fruiting body. To the best of our knowledge, this was the first report on the GC-AG introns in gpd gene. The splicing characterization of glycerol-3-phosphate dehydrogenase GC-AG introns in genus Agrocybe will provide help for further understanding of GC-AG intron in function and evolution.


Chen W.-M.,Yunnan Academy of Agricultural Sciences | Chen W.-M.,Yunnan Provincial Key Laboratory of Agricultural Biotechnology | Chen W.-M.,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation | Zhang X.-L.,Yunnan Academy of Agricultural Sciences | And 13 more authors.
Current Microbiology | Year: 2015

The large number of spores produced by edible mushrooms cause many problems, including causing lung disease, depleting natural genetic diversity, and reduced quality of fruiting bodies. Obtaining spore-deficient strains and understanding the underlying molecular mechanisms of such strains are important for breeding work. In this study, we crossed monokaryotic strains isolated from the edible fungi Agrocybe salicacola to obtain three spore-deficient strains with losses of the sterigmata on the surface of the lamella. A mating test revealed that recessive alleles distributed in some strains might control sterigmata development during the mitotic or meiotic phases. Transcriptome analysis revealed that the majority of the genes involved in DNA mismatch repair, base excision repair, and homologous recombination exhibited down-regulated expression patterns in the mutant fruiting bodies. Five genetic fragments, which were highly similar to the GTP-cyclohydrolase encoding gene, the DNA repair gene rad 8, and cell wall integrity and stress response component-encoding genes, were all expressed exclusively in the wild-type strains; these findings provide important information for the study of the spore development of edible fungi. © 2015, Springer Science+Business Media New York.


Xu F.,Yunnan Academy of Agricultural Sciences | Xu F.,Yunnan Provincial Key Laboratory of Agricultural Biotechnology | Xu F.,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation | A X.,Yunnan Academy of Agricultural Sciences | And 21 more authors.
Genetic Resources and Crop Evolution | Year: 2014

Due to the rapid diffusion of improved crop varieties, fewer and fewer landraces are being grown by farmers. The on-farm conservation of crop landraces has been proposed as means of conserving potentially important crop germplasm as an alternative to its ex situ conservation in gene banks, but the effectiveness of this approach is unproven. Particular attention is currently focused on producers sited in remote regions. Here, we report the outcomes of a survey focusing on the conservation and utilization of landraces of corn, rice, wheat, barley, buckwheat, broomcorn sorghum, Job's tears (coix), oats and finger, foxtail, broomcorn and barnyard millets grown by 15 ethnic groups from Yunnan province (China). Many local varieties are still in existence through their utilization on-farm. The varietal richness per village sampled was estimated to be 3.5 (maximum of 17), with rice and maize being the most heterogeneous, and glutinous sorghum and barnyard millet the least. Varietal richness was significantly and positively correlated with the number of villages surveyed, the number of families and the head of population. The choice of crops and varieties maintained varied between the ethnic groups, with the more westerly and north westerly situated villages conserving the most landraces. The number of crop species used was negatively correlated with per capita annual income, while the correlation coefficient between varietal richness and per capita annual income was less pronounced. The major factors determining the level of on-farm conservation were remoteness, fragmentation of the arable area and cultural needs. The data provide baseline information for the elaboration of optimal strategies for in situ conservation and utilization of crop germplasm in China. © The Author(s) 2013.

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