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Ma J.,Nanjing Agricultural University | Ma J.,Longyan University | Hou X.,Nanjing Agricultural University | Hou X.,Key Laboratory of Southern Vegetable Crop Genetic Improvement | And 5 more authors.
Plant Molecular Biology Reporter

A cDNA clone for the turnip mosaic virus (TuMV)-induced gene in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino cv. Duanbaigeng) was isolated and characterized. The full-length cDNA clone, designated BcTuR3, was isolated during resistance response to TuMV. Sequence analysis of the cDNA clone confirmed that the translation product of the gene is homologous to other plant resistance proteins. Genomic DNA Southern blot analysis indicated that the gene represented a small multi-gene family. Northern hybridizations confirmed its elevated expression in the resistant "Duanbaigeng" and the susceptible "Aijiaohuang." Upon inoculation with TuMV, the BcTuR3 transcript was rapidly accumulated in the infected leaves and stems. Tissue-specific expression of the gene showed that higher levels of the BcTuR3 transcript were observed in the TuMV-infected leaves, and later in the stems and roots of the resistant and susceptible varieties. These data showed that the BcTuR3 gene may be involved in plant resistance against TuMV pathogen infection. © Springer-Verlag 2010. Source

Wang X.H.,Nanjing Agricultural University | Wang X.H.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Song Y.P.,Nanjing Agricultural University | Song Y.P.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | And 10 more authors.
Journal of Animal and Plant Sciences

Non-heading Chinese cabbage is one of the main vegetables in Asia, especially in China. To analyze the tolerance of non-heading Chinese cabbage to copper (Cu) stress, 10μM, 100μM and 1mM copper were used to treat two cultivars of 'Wutacai' as well as 'Erqing' and then photosynthesis, chlorophyll fluorescence parameters, biomass and chlorophyll content were investigated. In our study, 1mM copper significantly inhibited the photosynthesis and plant growth of both two cultivars. While at 100μM copper, the growth inhibition was just found in 'Erqing', not in 'Wutacai'. The inhibition of copper on the photosynthesis was not only by stomatal factors but also by non-stomatal factors. Copper stress caused damage of photosystem resulting in reduction of PSII efficiency while non-photochemical-quenching parameter NPQ increased. Besides, at low concentration with 10μM, copper promoted the photosynthesis and plant growth. Therefore, the copper tolerance of non-heading Chinese cabbage was dependent on the genotype, and the plant growth inhibition under copper stress was due to the damage of photosystem. Our results will provide a theoretical basis for a further study on non-heading Chinese cabbage tolerance to copper and will be helpful for the production of non-heading Chinese cabbage in heavy metals contaminated areas. Source

Wang L.,Nanjing Agricultural University | Wang L.,State Key Laboratory of Crop Genetics | Peng H.,Nanjing Agricultural University | Peng H.,State Key Laboratory of Crop Genetics | And 9 more authors.
Plant Biology

Non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino), an important vegetable crop in China, exhibits a typical sporophytic self-incompatibility (SI) system. To better understand the mechanism of SI response and identify potential candidate proteins involved in the SI system of this vegetable crop, the proteomic approach was taken to identify differential accumulating pistil proteins. Pistils were collected at 0 h and 2 h after self-pollination at anthesis in self-incompatible and compatible lines of non-heading Chinese cabbage, and total proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE). A total of 25 protein spots that displayed differential abundance were identified by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF/TOF MS) and peptide mass fingerprinting (PMF). Among them, 22 protein spots were confidently established. The mRNA levels of the corresponding genes were detected by quantitative RT-PCR. The 22 identified protein spots are involved in energy metabolism (four), protein biosynthesis (three), photosynthesis (six), stress response and defence (five), and protein degradation (four). Among these potential candidate proteins, UDP-sugar pyrophosphorylase could be involved in sucrose degradation to influence pollen germination and growth. Glutathione S-transferases could be involved in pollen maturation, and affect pollen fertility. Senescence-associated cysteine protease, which is related to programmed cell death, could be mainly related to self pollen recognition of non-heading Chinese cabbage. The study will contribute to further investigations of molecular mechanism of sporophytic SI in Brassicaceae. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands. Source

Wang Z.,Nanjing Agricultural University | Wang Z.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Wang Z.,Key Laboratory of Southern Vegetable Crop Genetic Improvement | Jiang D.,Nanjing Agricultural University | And 18 more authors.

Turnip mosaic virus (TuMV) is the most prevalent viral pathogen infecting most cruciferous plants. MicroRNAs (miRNAs) are around 22 nucleotides long non-protein-coding RNAs that play key regulatory roles in plants. Recent research findings show that miRNAs are involved in plant-virus interaction. However we know little about plant defense and viral offense system networks throughout microRNA regulation pathway. In this study, two small RNA libraries were constructed based on non-heading Chinese cabbage (Brassica campestris ssp. chinensis L. Makino, NHCC) leaves infected by TuMV and healthy leaves, and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 86 conserved miRNAs belonging to 25 known miRNA families and 45 novel ones were identified. Among them, twelve conserved and ten new miRNAs were validated by real-time fluorescence quantitative PCR (qPCR). Differential expression analysis showed that 42 miRNAs were down-regulated and 27 miRNAs were up-regulated in response to TuMV stress. A total of 271 target genes were predicted using a bioinformatics approach, these genes are mainly involved in growth and resistance to various stresses. We further selected 13 miRNAs and their corresponding target genes to explore their expression pattern under TuMV and/or cold (4 °C) stresses, and the results indicated that some of the identified miRNAs could link TuMV response with cold response of NHCC. The characterization of these miRNAs could contribute to a better understanding of plant-virus interaction throughout microRNA regulation pathway. This can lead to finding new approach to defend virus infection using miRNA in Chinese cabbage. © 2015. Source

Jia Y.,Nanjing Agricultural University | Jia Y.,Key Laboratory of Southern Vegetable Crop Genetic Improvement | Jia Y.,Sichuan Agricultural University | Sun J.,Nanjing Agricultural University | And 5 more authors.
Chinese Journal of Applied Ecology

A water culture experiment was conducted to study the relationship between photosyn-thetic thermal dissipation and xanthophyll cycle in cucumber seedling leaves under hypoxia stress (the dissolved oxygen concentration in nutrient solution was 0.9-1.1 mg.L -1). Under the hypox-ia stress, there was a significant decrease in the quantum yield of PS -photochemistry rate (φPSII), net photosynthetic rate (Pn) under saturation light intensity, quanta yield (AQY), and maximal photochemical efficiency (Fv/Fm ), suggesting that the photoinhibition of the seedling leaves was induced. Meanwhile, the thermal dissipation (NPQ) and the allocation of dissipation energy (D) by antenna increased, but the photochemical quenching apparent (q P) decreased, sug- gesting the enhancement of thermal dissipation in cucumber leaves under hypoxia stress. A positive correlation was observed between NPQ and xanthophyll de-epoxidation state (DEPS), and both of them were promoted by ascorbic acid (AsA) and inhibited by 1,4-dithiothreitol (DTT), suggesting that xanthophyll cycle was the major pathway of photosynthetic thermal dissipation in cucumber seedling leaves under hypoxia stress. Source

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