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Li C.-R.,Anhui Academy of Agricultural Sciences | Li C.-R.,Key Laboratory of Rice Genetics and Breeding Ministry of Agriculture | Li C.-R.,Anhui University | Liang D.-D.,Anhui Academy of Agricultural Sciences | And 19 more authors.
Plant, Cell and Environment | Year: 2013

Mitochondrial retrograde regulation (MRR) is the transduction of mitochondrial signals to mediate nuclear gene expression. It is not clear whether MRR is a common regulation mechanism in plant abiotic stress response. In this study, we analysed the early abiotic stress response of the rice OsAOX1 genes, and the induction of OsAOX1a and OsAOX1b (OsAOX1a/b) was selected as a working model for the stress-induced MRR studies. We found that the induction mediated by the superoxide ion (O2· -)-generating chemical methyl viologen was stronger than that of hydrogen peroxide (H2O2). The addition of reactive oxygen species (ROS) scavengers demonstrated that the stress induction was reduced by eliminating O2· -. Furthermore, the stress induction did not rely on chloroplast- or cytosol-derived O2· -. Next, we generated transgenic plants overexpressing the superoxide dismutase (SOD) gene at different subcellular locations. The results suggest that only the mitochondrial SOD, OsMSD, attenuated the stress induction of OsAOX1a/b specifically. Therefore, our findings demonstrate that abiotic stress initiates the MRR on OsAOX1a/b and that mitochondrial O2· - is involved in the process. © 2012 Blackwell Publishing Ltd.

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