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Niu K.,Education Ministry Key Laboratory of Resource Chemistry | Huang C.,Education Ministry Key Laboratory of Resource Chemistry | Xu K.,Education Ministry Key Laboratory of Resource Chemistry | Zhi Y.,Education Ministry Key Laboratory of Resource Chemistry | And 2 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2014

An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 103 CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis. Copyright © 2014 American Scientific Publishers All rights reserved.

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