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Liu Q.,Tarim University | Liu Q.,Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang | Ren M.,Tarim University | Ren M.,Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

Two halophilic archaeal strains, TRM20010T and TRM20345T, were isolated from saline soil of the Lop Nur region in Xinjiang, north-west China. Cells from the two strains were pleomorphic rods, stained Gram-negative and produced red-pigmented colonies. Strains TRM20010T and TRM20345T were able to grow at 30–62 6C (optimum 37 6C), 0.9–5.1 M NaCl (optimum 2.6 and 3.4 M, respectively) and pH 6.0–10.0 (optimum pH 7.0”7.5) and neither strain required Mg2+ for growth. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), two glycolipids chromatographically identical to galactosyl mannosyl glucosyl diether (TGD-1) and disulfated mannosyl glucosyl diether (S2-DGD). Phylogenetic analysis based on 16S rRNA and rpoB9 genes revealed that strains TRM20010T and TRM20345T clustered together and formed a distinct clade separated from the related genera Halovivax, Haloterrigena, Halostagnicola, Natronolimnobius and Natrinema. The DNA G+C contents of strains TRM20010T and TRM20345T were 63.9 and 63.8 mol%, respectively. The DNA–DNA hybridization value between strain TRM20010T and strain TRM20345T was 42.8%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strains TRM20010T and TRM20345T represent two novel species in a new genus within the family Halobacteriaceae, for which the names Natribaculum breve gen. nov., sp. nov. (type strain TRM20010T5CCTCC AB2013112T5NRRL B-59996T) and Natribaculum longum sp. nov. (type strain TRM20345T5CCTCC AB2013113T5NRRL B-59997T) are proposed. ©2015 IUMS. Source

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