Entity

Time filter

Source Type


Yu Z.-H.,ShenYang Agricultural University | Cui N.,ShenYang Agricultural University | Dong X.-F.,ShenYang Agricultural University | Han M.-L.,ShenYang Agricultural University | And 3 more authors.
International Journal of Agriculture and Biology | Year: 2012

SPS is a target for 14-3-3s and there are 12 isoforms (TFT1-TFT12) of 14-3-3s in tomato (Solanum lycopersicum). Bornke's research showed that T14-3d and T14-3g, isoforms of 14-3-3s, can interact with SPSA in tobacco (Nicotiana tabacum). Analyzing the physical and chemical properties of 14-3-3s, 3-D structures and motifs of tomato 14-3-3s and SPS with bioinformatics softwares, we predicted, which isoform(s) has (have) the ability to bind with SPS based on Bornke's research. The results showed that aliphatic indexes are all greater than 70 and the grand average of hydrophobicity ranged from -0.701 to -0.269 of 14-3-3s in tomato, indicating that tomato 14-3-3s are hydrophilic proteins with high thermal stability. The similarities between TFT1 and T14-3d, TFT10 and T14-3 g had 95% and 94% identity, respectively. By constructing a phylogenetic tree, TFT1\T14-3d\TFT10\T14-3 g are found to be on the same branch. This indicated that tft1 and t14-3d, tft10 and t14-3 g are 2 groups of orthologous genes. The 3-D structure modeling showed that TFT1 and T14-3d, TFT10 and T14-3 g have similar structures. Likewise, structures of tomato SPS and tobacco SPSA were very similar. Moreover, all tomato 14-3-3s had an auto-inhibitory domain in C-terminal variable region to prevent the interaction between 14-3-3s and SPS with the exception of TFT1 and TFT10. From these comprehensive analyses, we clearly deduced that TFT1 and TFT10 are those most likely to interact with SPS in tomato. © 2012 Friends Science Publishers.


Yu N.-H.,ShenYang Agricultural University | Cui N.,ShenYang Agricultural University | Cui N.,Key Laboratory of Protected Horticulture of Liaoning Province | Dong X.-F.,ShenYang Agricultural University | And 4 more authors.
African Journal of Biotechnology | Year: 2011

Tomato cv. Liaoyuanduoli (Solanum lycopersicum) plants were cultivated in a greenhouse to allow sampling of the second fruit in the first cluster and comparison with tomato fruit that developed following para-chlorophenoxyacetic acid (PCPA) treatment. Sugar content, activities of sugar related enzymes and the effects of PCPA treatment on gene expression of soluble acid invertase (AI) during tomato fruit development were studied. Enhanced activity of AI and increased gene expression of soluble AI in pectinic tissues, pericarp and dissepiments were observed during fruit development, resulting in an increase in fructose and glucose levels. Following PCPA treatment, the activity of AI was amplified and gene expression of soluble AI was accelerated during the mature period of fruit so that fructose and glucose were increased. Upon ripening and following PCPA treatment, the activity of AI and gene expression of soluble AI in pectinic tissues were more pronounced than those in pericarp and dissepiments, but the concentrations of fructose and glucose in pectinic tissues were lower than in pericarp and dissepiments. PCPA appeared to affect vacuolar acid invertase activity by regulating corresponding gene expression, resulting in hexose accumulation in ripening tomato fruit. © 2011 Academic Journals.


Liu Y.F.,ShenYang Agricultural University | Liu Y.F.,Key Laboratory of Protected Horticulture of Ministry of Education | Liu Y.F.,Key Laboratory of Protected Horticulture of Liaoning Province | Qi M.F.,ShenYang Agricultural University | And 5 more authors.
Plant Science | Year: 2012

The effects of low night temperature (LNT, i.e., 9 and 6°C) stress and rewarming (15°C night temperature) on the photosynthesis, photosystems I and II (PSI and PSII), and antioxidant system of tomato leaves were studied. The results showed that 9 d of LNT treatment led to an irreversible reduction in the photosynthetic rate. This reduction was accompanied by stomatal limitation of CO2 supply and significant decline in ribulose-1,5-bisphosphate carboxylase/oxygenase activity at the transcription level, as well as sucrose accumulation. LNT treatment induced the reversible photoinhibition of PSII, decreased PSII activity, increased the photochemical yield of PSI Y(I), and markedly caused the acceptor side limitation of PSI. This finding was reflected by the higher value of Y(NA) in the treated plants than in the control. At the same time, a downregulation of electron transport for photosynthetic carbon reduction under LNT was mostly compensated by Ja(O2-dependent) driven by the water-water cycle. © 2012.


Qi M.,ShenYang Agricultural University | Qi M.,Key Laboratory of Protected Horticulture | Qi M.,Key Laboratory of Protected Horticulture of Liaoning Province | Liu Y.,ShenYang Agricultural University | And 5 more authors.
Biological Trace Element Research | Year: 2013

Nano-TiO2 has been reported to promote photosynthesis in some crops; however, the mechanism behind this action remains unknown. In this research, the effects of nano-TiO2 on leaf photosynthesis under mild heat stress were investigated. Results showed that the net photosynthetic rate, conductance to H2O, and transpiration rate of tomato leaves increased after application of an appropriate concentration of nano-TiO2. Nano-TiO2 also significantly decreased the minimum chlorophyll fluorescence and relative electron transport in leaves. Under mild heat stress, Nano-TiO2 increased regulated photosystem II (PS II) energy dissipation and decreased non-regulated PS II energy dissipation. These results indicate that nano-TiO2 plays a positive role in promoting photosynthesis in tomato leaves under mild heat stress. © 2013 Springer Science+Business Media New York.


Liu Y.-F.,ShenYang Agricultural University | Liu Y.-F.,Key Laboratory of Protected Horticulture of Ministry of Education | Liu Y.-F.,Key Laboratory of Protected Horticulture of Liaoning Province | Zhang G.-X.,ShenYang Agricultural University | And 8 more authors.
Journal of Plant Growth Regulation | Year: 2015

The effect of exogenous CaCl2 on photosynthesis, antioxidant system, and chloroplast ultrastructure of tomato leaves under low night temperature (LNT) was investigated. Tomato seedlings were pretreated with 27 mM CaCl2, 5 mM EGTA, respectively, for 4 days and then exposed to LNT treatment at 6 °C for 7 days. Plants treated with CaCl2 showed higher photosynthesis, increased stomatal aperture and chloroplast area, and decreased number of starch grains under LNT stress than those treated with distilled water. LNT increased the contents of superoxide radicals, hydrogen peroxide, and malondialdehyde, whereas calcium application reduced the contents of these compounds. The increase in antioxidant activities caused by LNT stress was also enhanced by the application of exogenous calcium. The opposite effects were observed with ethylene glycol-bis-(2-aminoethyl) tetraacetic acid pretreatment. CaCl2 application improved photosynthesis in LNT-stressed plants. Such improvement was associated with increased stomatal conductance, improved antioxidant activities, and adjusted chloroplast structure. These results suggest that CaCl2 application improved photosynthesis in LNT-stressed plants. It provided fundamental information for further molecular mechanism research. © 2014, Springer Science+Business Media New York.

Discover hidden collaborations