Key Laboratory of Protected Horticulture of Liaoning Province

Nomhon, China

Key Laboratory of Protected Horticulture of Liaoning Province

Nomhon, China
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Fu H.,ShenYang Agricultural University | Fu H.,Key Laboratory of Protected Horticulture of Ministry of Education | Fu H.,Key Laboratory of Protected Horticulture of Liaoning Province | Zhang G.,ShenYang Agricultural University | And 12 more authors.
Sustainability (Switzerland) | Year: 2017

Soil-related obstacles resulting from continuous monoculture have limited the sustainable development of the tomato industry in China. An experiment on tomatoes with seven continuous monoculture treatments (the 1st, 3rd, 5th, 7th, 9th, 11th, and 13th crops, respectively) was conducted in a solar greenhouse, to investigate the influence of monoculture on soil quality. Most soil quality indicators first increased and then decreased with increasing continuous monoculture crops, and significant differences among crops were observed. Indicators at the 13th crop were significantly lower than those at the other crops in terms of average well color development (AWCD), substrate richness (S), the Shannon diversity index (H), and the McIntosh index (U) of the soil microbial community (SMC), soil urease (UR), and neutral phosphatase (N-PHO) activities, and available nitrogen (AN) and potassium (AK). However, fungal abundance (FUN) at the 13th crop was significantly higher than that at the other crops. As principal component analysis (PCA) revealed, SMC functional diversity at the 1st, 11th, and 13th crops were similar, and were obviously distinguished from those at the other crops. Moreover, the tomato yield was significantly and positively correlated with soil-available potassium and SMC functional diversity indexes. Our findings indicated that short-term continuous monoculture, e.g., for fewer than seven or nine crops, was beneficial for soil quality improvement. However, continuous monoculture for greater than 11 crops had adverse effects on soil enzyme activities, soil microbial abundances, soil chemical properties, soil SMC functional diversity, and the tomato yield, particularly at the 13th crop. © 2017 by the author.

Sun Z.,Key Laboratory of Protected Horticulture | Sun Z.,ShenYang Agricultural University | Sun Z.,Key Laboratory of Protected Horticulture of Liaoning Province | Huang W.,Lingyuan Hongyuan Protected Agricultural Service Co. | And 11 more authors.
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2013

The Chinese energy-saving solar greenhouse (CESG) is a special type of greenhouse without heating system, originated in Anshan, Liaoning province. In China, during the mid-1980s, the CESG was composed of the front house roof (for lighting), north wall, east-west gable, back roof (for heat storage and heat preservation), and outside heat preservation covering materials. The applied area of CESG in China reached 928 thousand hm2 by 2012, which has completely resolved the problem on the year-round vegetable supply, especially in winter in north China. However, the CESG also faces many problems such as uneven distribution of light and temperature in greenhouse, difficulties to achieve automatic control of outside heat preservation covering materials, poor ability of rainproof, snow-proof, wind-proof and fire-proof, and destruction of lots of arable land for greenhouse construction of earth wall and brick wall. To resolve the above problems, a new type of energy-saving solar greenhouse assembled with heat preservation color plate (CPSG) was developed in Lingyuan (latitude 41°20'N, longitude 119°31'E), Liaoning province, China from 2010 to 2012. The light and temperature performances were studied from January to March, 2013.The CPSG was the large scale arched structure with a span of 12 m, a ridge height of 5.5 m, a length of 65m, a front roof lighting angle of 41.5°, a front roof covering materials of polyolefin film. The skeleton structure was semi-circular arc, which slide to opening or closing the heat preservation covering materials of rock wool color plate outside, and to move the opening or closing of the rock wool color plate east gable (in the morning) and west gable (in the afternoon) respectively. As a result, the CPSG not only resolved the problem of rain-proof, snow-proof, wind-proof and fire-proof for Chinese traditional solar greenhouse, but also brought about the accurate operation of outside heat preservation color plate. Furthermore, this type of greenhouse adopted water-recycle heat storage-release system (WHSS) and air-underground heat exchange storage-release system (AHESS). WHSS is running 5.5h/d on sunny day and the solar energy storage capacity is 1200 kJ/m2 (greenhouse area), which made the greenhouse temperature increase 7-8°C in winter night. At the same time, solar energy accumulation for AHESS is 221 kJ/m2, which shows that two heat storage-release systems may replace the heat storage-release function of earth wall and brick wall, and then to lay the foundation for assembled construction of the CESG. Compared with the traditional Liaoshen III type solar greenhouse, CPSG increases 5.3% of the lighting quantity by enhancing 16.3° in the front roof lighting angle. When the outside nighttime air temperature was -25.8°C, the nighttime air temperature in the CPSG reaches more than 13°C with an increase of 2.3-3.5°C, the temperature difference between inside and outside reaches 39.1°C. In addition, the CPSG has larger cultivation space, better lighting quantity, more rapid warming up, and more even distribution of light and temperature in the north-south direction or the east-west direction, resulting in a unanimous plant growth. In summary, the study shows that new type of CPSG integrates the advantages of the large multi-span greenhouse and Chinese traditional solar greenhouse, heightens the efficiency of light energy utilization, reduces the labor intensity, realizes the accurate control of heat preservation covering materials, will provide a new effective way for automation and modernization of solar greenhouse in China.

Liu X.,ShenYang Agricultural University | Liu X.,Key Laboratory of Protected Horticulture of Ministry of Education | Liu X.,Key Laboratory of Protected Horticulture of Liaoning Province | Dong X.,ShenYang Agricultural University | And 20 more authors.
Plant Molecular Biology | Year: 2016

Solanum lycopersicum auxin response factor 10 (SlARF10) is post-transcriptionally regulated by Sl-miR160. Overexpression of a Sl-miR160-resistant SlARF10 (mSlARF10) resulted in narrower leaflet blades with larger stomata but lower densities. 35S:mSlARF10-6 plants with narrower excised leaves had greater water loss, which was in contrast to the wild type (WT). Further analysis revealed that the actual water loss was not consistent with the calculated stomatal water loss in 35S:mSlARF10-6 and the WT under the dehydration treatment, indicating that there is a difference in hydraulic conductance. Pretreatment with abscisic acid (ABA) and HgCl2 confirmed higher hydraulic conductance in 35S:mSlARF10, which is related to the larger stomatal size and higher activity of aquaporins (AQPs). Under ABA treatment, 35S:mSlARF10-6 showed greater sensitivity, and the stomata closed rapidly. Screening by RNA sequencing revealed that five AQP-related genes, fourteen ABA biosynthesis/signal genes and three stomatal development genes were significantly altered in 35S:mSlARF10-6 plants, and this result was verified by qRT-PCR. The promoter analysis showed that upregulated AQPs contain AuxRE and ABRE, implying that these elements may be responsible for the high expression levels of AQPs in 35S:mSlARF10-6. The three most upregulated AQPs (SlTIP1-1-like, SlPIP2;4 and SlNIP-type-like) were chosen to confirm AuxRE and ABRE function. Promoters transient expression demonstrated that the SlPIP2;4 and SlNIP-type-like AuxREs and SlPIP2;4 and SlTIP1-1-like ABREs could significantly enhance the expression of the GUS reporter in 35S:mSlARF10-6, confirming that AuxRE and ABRE may be the main factors inducing the expression of AQPs. Additionally, two upregulated transcription factors in 35S:mSlARF10-6, SlARF10 and SlABI5-like were shown to directly bind to those elements in an electromobility shift assay and a yeast one-hybrid assay. Furthermore, transient expression of down-regulated ARF10 or up-regulated ABI5 in tomato leaves demonstrated that ARF10 is the direct factor for inducing the water loss in 35S:mSlARF10-6. Here, we show that although SlARF10 increased the ABA synthesis/signal response by regulating stomatal aperture to mitigate water loss, SlARF10 also influenced stomatal development and AQP expression to affect water transport, and both act cooperatively to control the loss of leaf water in tomato. Therefore, this study uncovers a previously unrecognized leaf water loss regulatory factor and a network for coordinating auxin and ABA signalling in this important process. In an evolutionary context, miR160 regulates ARF10 to maintain the water balance in the leaf, thus ensuring normal plant development and environmental adaptation. © 2016 Springer Science+Business Media Dordrecht

Liu Y.F.,ShenYang Agricultural University | Liu Y.F.,Key Laboratory of Protected Horticulture of Ministry of Education | Liu Y.F.,Key Laboratory of Protected Horticulture of Liaoning Province | Qi M.F.,ShenYang Agricultural University | And 5 more authors.
Plant Science | Year: 2012

The effects of low night temperature (LNT, i.e., 9 and 6°C) stress and rewarming (15°C night temperature) on the photosynthesis, photosystems I and II (PSI and PSII), and antioxidant system of tomato leaves were studied. The results showed that 9 d of LNT treatment led to an irreversible reduction in the photosynthetic rate. This reduction was accompanied by stomatal limitation of CO2 supply and significant decline in ribulose-1,5-bisphosphate carboxylase/oxygenase activity at the transcription level, as well as sucrose accumulation. LNT treatment induced the reversible photoinhibition of PSII, decreased PSII activity, increased the photochemical yield of PSI Y(I), and markedly caused the acceptor side limitation of PSI. This finding was reflected by the higher value of Y(NA) in the treated plants than in the control. At the same time, a downregulation of electron transport for photosynthetic carbon reduction under LNT was mostly compensated by Ja(O2-dependent) driven by the water-water cycle. © 2012.

Liu Y.-F.,ShenYang Agricultural University | Liu Y.-F.,Key Laboratory of Protected Horticulture of Ministry of Education | Liu Y.-F.,Key Laboratory of Protected Horticulture of Liaoning Province | Zhang G.-X.,ShenYang Agricultural University | And 8 more authors.
Journal of Plant Growth Regulation | Year: 2015

The effect of exogenous CaCl2 on photosynthesis, antioxidant system, and chloroplast ultrastructure of tomato leaves under low night temperature (LNT) was investigated. Tomato seedlings were pretreated with 27 mM CaCl2, 5 mM EGTA, respectively, for 4 days and then exposed to LNT treatment at 6 °C for 7 days. Plants treated with CaCl2 showed higher photosynthesis, increased stomatal aperture and chloroplast area, and decreased number of starch grains under LNT stress than those treated with distilled water. LNT increased the contents of superoxide radicals, hydrogen peroxide, and malondialdehyde, whereas calcium application reduced the contents of these compounds. The increase in antioxidant activities caused by LNT stress was also enhanced by the application of exogenous calcium. The opposite effects were observed with ethylene glycol-bis-(2-aminoethyl) tetraacetic acid pretreatment. CaCl2 application improved photosynthesis in LNT-stressed plants. Such improvement was associated with increased stomatal conductance, improved antioxidant activities, and adjusted chloroplast structure. These results suggest that CaCl2 application improved photosynthesis in LNT-stressed plants. It provided fundamental information for further molecular mechanism research. © 2014, Springer Science+Business Media New York.

Zhang G.,ShenYang Agricultural University | Zhang G.,Key Laboratory of Protected Horticulture of Ministry of Education | Zhang G.,Key Laboratory of Protected Horticulture of Liaoning Province | Liu Y.,ShenYang Agricultural University | And 14 more authors.
PLoS ONE | Year: 2014

The effect of exogenous CaCl2 on photosystem I and II (PSI and PSII) activities, cyclic electron flow (CEF), and proton motive force of tomato leaves under low night temperature (LNT) was investigated. LNT stress decreased the net photosynthetic rate (Pn), effective quantum yield of PSII [Y(II)], and photochemical quenching (qP), whereas CaCl2 pretreatment improved Pn, Y(II), and qP under LNT stress. LNT stress significantly increased the non-regulatory quantum yield of energy dissipation [Y(NO)], whereas CaCl 2 alleviated this increase. Exogenous Ca2+ enhanced stimulation of CEF by LNT stress. Inhibition of oxidized PQ pools caused by LNT stress was alleviated by CaCl2 pretreatment. LNT stress reduced zeaxanthin formation and ATPase activity, but CaCl2 pretreatment reversed both of these effects. LNT stress caused excess formation of a proton gradient across the thylakoid membrane, whereas CaCl2 pretreatment decreased the said factor under LNT. Thus, our results showed that photoinhibition of LNT-stressed plants could be alleviated by CaCl2 pretreatment. Our findings further revealed that this alleviation was mediated in part by improvements in carbon fixation capacity, PQ pools, linear and cyclic electron transports, xanthophyll cycles, and ATPase activity. © 2014 Zhang et al.

Qi M.,ShenYang Agricultural University | Qi M.,Key Laboratory of Protected Horticulture | Qi M.,Key Laboratory of Protected Horticulture of Liaoning Province | Liu Y.,ShenYang Agricultural University | And 5 more authors.
Biological Trace Element Research | Year: 2013

Nano-TiO2 has been reported to promote photosynthesis in some crops; however, the mechanism behind this action remains unknown. In this research, the effects of nano-TiO2 on leaf photosynthesis under mild heat stress were investigated. Results showed that the net photosynthetic rate, conductance to H2O, and transpiration rate of tomato leaves increased after application of an appropriate concentration of nano-TiO2. Nano-TiO2 also significantly decreased the minimum chlorophyll fluorescence and relative electron transport in leaves. Under mild heat stress, Nano-TiO2 increased regulated photosystem II (PS II) energy dissipation and decreased non-regulated PS II energy dissipation. These results indicate that nano-TiO2 plays a positive role in promoting photosynthesis in tomato leaves under mild heat stress. © 2013 Springer Science+Business Media New York.

Tong X.,Key Laboratory of Protected Horticulture | Tong X.,Key Laboratory of Protected Horticulture of Liaoning Province | Tong X.,Key Laboratory of Protected Vegetables Engineering of Liaoning Province | Tong X.,ShenYang Agricultural University | And 12 more authors.
Taiyangneng Xuebao/Acta Energiae Solaris Sinica | Year: 2016

Researching on heating effects of solar energy water-cycling system in solar greenhouse in winner and summer, solar energy was regarded as support, water was used as media to store and release heat, and PC board was used as the device to store and release heat. We tested the heat storage of different color of PC board, different thickness of PC board and different flow speed of PC board respectively. And compare with heating effect and heating efficiency of solar energy water-cycling system between a fine day in winter and in summer. The experiment showed that the heat storage of 6 mm brown PC board was 191.5 kJ/(m2·d), the most. It increased by 15.2% than transparent PC board. And the heat storage of 8 mm transparent PC board was 198.2 kJ/(m2 ·d), it increased by 19.2% and 4.5% than 6 mm and 10 mm PC board respectively. Then when the water flow was 4.4-4.5 L/h, the heat storage of PC board was the most, heating effect was also the best. The heat storage of solar energy water- cycling system reached 159.8 MJ, it made the greenhouse temperature 3-5℃ higher, and the heating efficiency was 54.5% in winter. At the same time, the solar energy water-cycling system made the greenhouse temperature 5-8℃ lower, and the heating efficiency was 54.6% in summer. So the water-cycling system can not only increase the utilization of the solar energy, but also make the solar greenhouse high efficiency and energy saving, and provide the foundation for the development of prefabricated sunlight greenhouse structure. © 2016, Editorial Board of Acta Energiae Solaris Sinica. All right reserved.

Yu Z.-H.,ShenYang Agricultural University | Cui N.,ShenYang Agricultural University | Dong X.-F.,ShenYang Agricultural University | Han M.-L.,ShenYang Agricultural University | And 3 more authors.
International Journal of Agriculture and Biology | Year: 2012

SPS is a target for 14-3-3s and there are 12 isoforms (TFT1-TFT12) of 14-3-3s in tomato (Solanum lycopersicum). Bornke's research showed that T14-3d and T14-3g, isoforms of 14-3-3s, can interact with SPSA in tobacco (Nicotiana tabacum). Analyzing the physical and chemical properties of 14-3-3s, 3-D structures and motifs of tomato 14-3-3s and SPS with bioinformatics softwares, we predicted, which isoform(s) has (have) the ability to bind with SPS based on Bornke's research. The results showed that aliphatic indexes are all greater than 70 and the grand average of hydrophobicity ranged from -0.701 to -0.269 of 14-3-3s in tomato, indicating that tomato 14-3-3s are hydrophilic proteins with high thermal stability. The similarities between TFT1 and T14-3d, TFT10 and T14-3 g had 95% and 94% identity, respectively. By constructing a phylogenetic tree, TFT1\T14-3d\TFT10\T14-3 g are found to be on the same branch. This indicated that tft1 and t14-3d, tft10 and t14-3 g are 2 groups of orthologous genes. The 3-D structure modeling showed that TFT1 and T14-3d, TFT10 and T14-3 g have similar structures. Likewise, structures of tomato SPS and tobacco SPSA were very similar. Moreover, all tomato 14-3-3s had an auto-inhibitory domain in C-terminal variable region to prevent the interaction between 14-3-3s and SPS with the exception of TFT1 and TFT10. From these comprehensive analyses, we clearly deduced that TFT1 and TFT10 are those most likely to interact with SPS in tomato. © 2012 Friends Science Publishers.

Yu N.-H.,ShenYang Agricultural University | Cui N.,ShenYang Agricultural University | Cui N.,Key Laboratory of Protected Horticulture of Liaoning Province | Dong X.-F.,ShenYang Agricultural University | And 4 more authors.
African Journal of Biotechnology | Year: 2011

Tomato cv. Liaoyuanduoli (Solanum lycopersicum) plants were cultivated in a greenhouse to allow sampling of the second fruit in the first cluster and comparison with tomato fruit that developed following para-chlorophenoxyacetic acid (PCPA) treatment. Sugar content, activities of sugar related enzymes and the effects of PCPA treatment on gene expression of soluble acid invertase (AI) during tomato fruit development were studied. Enhanced activity of AI and increased gene expression of soluble AI in pectinic tissues, pericarp and dissepiments were observed during fruit development, resulting in an increase in fructose and glucose levels. Following PCPA treatment, the activity of AI was amplified and gene expression of soluble AI was accelerated during the mature period of fruit so that fructose and glucose were increased. Upon ripening and following PCPA treatment, the activity of AI and gene expression of soluble AI in pectinic tissues were more pronounced than those in pericarp and dissepiments, but the concentrations of fructose and glucose in pectinic tissues were lower than in pericarp and dissepiments. PCPA appeared to affect vacuolar acid invertase activity by regulating corresponding gene expression, resulting in hexose accumulation in ripening tomato fruit. © 2011 Academic Journals.

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