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Bao H.,Liaoning University | Bao H.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | Ma X.,Liaoning University | Ma X.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | And 7 more authors.
Applied Mechanics and Materials | Year: 2012

The objective of this study is to investigate the microbial community and its characteristics changes aroused by the ratios of COD/SO 4 2- in the acidogenic-phase reactor of twophase anaerobic process. A continuous-flow lab-scale test was conducted in an acidogenic sulfatereducing reactor with molasses wastewater as sole organic carbon source and sodium sulfate as the electron acceptor. The experimental results showed that Acetic-acid Type Microbial Metabolism resulted in the formation of An Acetic-acid Type Climax Community. The change of the COD/SO 4 2- ratio caused an ecological succession from a stable climax community at moderate and high COD/SO 4 2- ratios to a sub-stable climax community at a lower COD/SO 4 2- ratio. But Aceticacid Type Microbial Metabolism kept unchanged during this course, which indicated the stability of Acetic-acid Type Climax Community. The Acetic-acid Type Microbial Metabolism and Acetic-acid Type Climax Community were of typical characteristics in the acidogenic sulfate-reducing reactor. © (2012) Trans Tech Publications.


Xue S.,Liaoning University | Xue S.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | Zhao Q.,Harbin Institute of Technology | Wei L.,Harbin Institute of Technology | And 2 more authors.
Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae | Year: 2010

Dissolved organic matter in secondary treated effluents from the W Wastewater Treatment Plant in the city of Harbin was fractionated using XAD resins into 5 fractions: hydrophobic acid (HPO-A), hydrophobic neutral (HPO-N), transphilic acid (TPI-A), transphilic neutral (TPI-N) and hydrophilic fraction (HPI). The fluorescence characteristics of each DOM fraction during chlorination were examined. The results showed that humic acid-, soluble microbial byproducts- and aromatic protein-like fluorescent materials in DOM were involved in chlorination. In addition, fulvic acid-like fluorescent materials in HPO-A, HPO-N, TPI-A and TPI-N, polycyclic aromatics with three to five fused benzene rings in HPO-A, HPO-N and TPI-A, as well as naphthalene with its derivatives in HPO-N reacted with chlorine. Fluorescent materials with polycyclic aromatic structures were produced in TPI-N and HPI, and fulvic acid-like fluorophores in HPI as well, as a result of chlorination. The wavelength that corresponds to the position of the normalized emission band at its half-intensity (for wavelengths > the wavelength at which the maximum emission intensity is observed) was measured in the fluorescence emission spectra (excitation at 320 nm) for each fraction decreased after chlorination, which was significant for TPI-N and HPI. But the phenomenon was not observed in the fluorescence emission spectra with excitation wavelengths of 280 nm and 370 nm.


Xue S.,Liaoning University | Zhao Q.-L.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | Wei L.-L.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | Li F.-Y.,Liaoning University | Ma X.-P.,Liaoning University
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2010

The changes of fluorescence characteristics of dissolved organic matter (DOM) during laboratory-scale soil-aquifer treatment (SAT) soil columns were studied. Using XAD resins, DOM was fractionated into 5 fractions: hydrophobic acid (HPO-A), hydrophobic neutral (HPO-N), transphilic acid (TPI-A), transphilic neutral (TPI-N) and hydrophilic fraction (HPI). Aromatic protein-like fluorescent materials in TPI-N were preferentially removed during SAT. The raletive content of aromatic protein- and soluble microbial byproduct-like fluorescent materials in HPO-A, HPO-N, TPI-A and HPI increased as a result of SAT. The removal rates of fluorescent materials with three to five fused benzene rings and humic acid-like fluorescent materials with excitation wavelength/emission wavelength (λ ex/λ em) of 390~ 410nm/456~476nm in each DOM fraction during SAT were lower than those of organics (on average) comprising dissolved organic carbon (DOC) in corresponding fraction. Other fluorescent materials in different fractions exhibited distinct removal behavior.


Bao H.,Liaoning University | Bao H.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | Ma X.,Liaoning University | Ma X.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | And 7 more authors.
Advanced Materials Research | Year: 2012

A sequencing batch reactors (SBR) was adopted to investigate the denitrifying phosphorus removal efficiency employing nitrite as electron acceptor under anaerobic/anoxic condition. The experimental results showed that high nitrogen and phosphorus removal efficiency could be obtained under the following conditions: nitrite concentration of 30∼40 mg/L, COD concentration of 400 mg/L, pH 8.0±0.2 in anaerobic stage and pH 7.2±0.2 in anoxic stage, sludge retention time (SRT) of 22 days. When the reactor performed steadily, a dominant functional strain was screened from the activated sludge, which has high nitrite and phosphorus removal efficiency. Batch tests results showed that the removal degree of nitrite and phosphorus could reach 99.18% and 84.94% respectively when their concentrations were 20mg/L and 10mg/L. according to the morphology and physio-biochemical characteristics, and the results of 16S rDNA sequencing, it is determined that the strain belongs to the Genus of Sphingobacterium. The experimental results achieved in this study might offer guidance to the development of shortcut denitrifying phosphorus removal process. © (2012) Trans Tech Publications, Switzerland.


Xue S.,Liaoning University | Xue S.,Key Laboratory of Pollution Control and Environmental Remediation of Liaoning Province | Zhao Q.,Harbin Institute of Technology | Ma X.,Liaoning University | And 6 more authors.
Environmental Monitoring and Assessment | Year: 2011

This research compared the structural and chemical characteristics among dissolved organic matter (DOM) fractions within the same source and among different origins. Samples taken from the Taiping Wastewater Treatment Plant (TWTP) (Harbin, China) and from the Songhuajing River (SR), Heilongjiang Province, China were chosen to represent waters containing DOM of wastewater origin and of natural-water origin, respectively. DOM was fractionated using XAD resins into five fractions: hydrophobic acid (HPO-A), hydrophobic neutral (HPO-N), transphilic acid (TPI-A), transphilic neutral (TPI-N) and hydrophilic fraction (HPI). The SR fractions were more UV-sensitive and more reactive with chlorine in formation of trihalomethanes (THMs) than the TWTP secondary effluent (TSE) fractions. The aromatic character peaks in the Fourier-transform infrared (FT-IR) spectra of SR fractions were clearer than those of TSE fractions. On the other hand, the peaks of carbohydrates in TSE fractions were more prominent in comparison with SR fractions. In addition, the amide-2 peak was present in the spectra of all the five TSE fractions but not visible in the spectra of SR fractions. The fluorescence results showed that SR DOM fractions contained more fulvic acid-like fluorescent compounds while TSE DOM fractions had higher amounts of protein-like fluorescent components. © 2010 Springer Science+Business Media B.V.

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