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Wu X.,Nanchang University | Wu X.,Key Laboratory of Plant Resources in Jiangxi Province | Guo X.,Nanchang University | Guo X.,Key Laboratory of Plant Resources in Jiangxi Province | And 7 more authors.
Journal of the Chinese Cereals and Oils Association | Year: 2013

With camphor seed oil(CSO)as raw material, the craft of CSO to produce diacylglycerol(DAG)under the lipase hydrolysis was optimized. With pretreatment of organic solvents acetone and tert-butanol, the best lipase was chose among three commercial immobilized lipases including Lipozyme RM IM, Lipozyme TL IM and Novozyme 435. Using quantity scores of DAG and Triacylglycerol(TAG)as the response values, and setting the water content(30%~50% of enzyme mass), enzyme content(8%~12% of oil mass)and reaction temperature(55~75°C)as the variables, the response surface methodology(RSM)was applied to optimize the process of producing DAG by hydrolysis of CSO. The results showed that Lipozyme RM IM could be reused for 11 times after being pretreated. The RSM optimal reaction conditions of DAG were: 40% of water content, 10% of enzyme load, 65°C of reaction temperature, and under this condition, the quantity scores of both DAG and TAG were 50.38% and 40.68%, respectively. This craft can provide theory and test values for the application of Commercialized production of the Medium Chain DAG from CSO. Source

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