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Wang J.,Key Laboratory of Plant Protection Resources and Pest Management
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

Bradysia hygida salivary glands antimicrobial peptide 1 (BhSGAMP-1) is one of the antimicrobial peptides involved in a preventive mechanism of defense of the fly Bradysia hygida. To know better about the molecular characterization of this antimicrobial peptide, we expressed and purified the modified BhSGAMP-1-S and investigated its antimicrobial activity. We synthesized the gene of BhSGAMP-1-S designed with preferred codons of Escherichia coli and expressed it as a fusion protein in E. coli TB1 by using pMAL-c2X as vector. We purified the fusion protein using amylase resin affinity chromatography. In addition, we cleaved the fusion protein by enterokinase and the released recombinant BhSGAMP-1-S was separated by size exclusion chromatography, then followed by reversed-phase high performance liquid chromatography. We analyzed the antimicrobial activities of the purified recombinant BhSGAMP-1-S by bioassays. The fusion protein was mostly expressed in soluble form under the optimized conditions. The recombinant BhSGAMP-1-S was produced with a pure yield of 0.38 mg/100 mL culture medium. Antimicrobial assays demonstrated that the recombinant BhSGAMP-1-S was active against several Gram-positive and Gram-negative bacteria and fungi. It appears to be the first successful production of the recombinant BhSGAMP-1-S from fly Bradysia hygida. Data presented here confirm that the recombinant BhSGAMP-1-S is now ready for further studying and characterizing their antimicrobial properties. Source


Yang Z.,Key Laboratory of Plant Protection Resources and Pest Management | Landry J.-F.,Agriculture and Agri Food Canada | Handfield L.,133 rue Messier and 301 | Zhang Y.,Key Laboratory of Plant Protection Resources and Pest Management | And 6 more authors.
Systematic Entomology | Year: 2012

Anania coronata (Hufnagel), a Holarctic species of pyraustine crambid moth, has long been treated as having two geographically separated subspecies - the nominotypical Anania coronata in the Palaearctic Region and Anania coronata tertialis (Guenée) in the Nearctic Region. Maximum likelihood and Bayesian inference analysis of mitochondrial DNA barcodes both recover four well-supported, reciprocally monophyletic groups within Anania coronata. Qualitative and quantitative analyses of genital structures reveal diagnostic differences that correspond to the four barcode lineages. On the basis of both molecular and morphological evidence, we conclude that Anania coronata is actually a complex of four species. Anania coronata (Hufnagel) is restricted to Europe, whereas three species occur in North America: Anania tertialis (Guenée), Anania plectilis (Grote & Robinson) and Anania tennesseensis sp.n. Yang. © 2012 The Authors. Systematic Entomology © 2012 The Royal Entomological Society. Source


Xue Q.,Key Laboratory of Plant Protection Resources and Pest Management | Webb M.D.,Natural History Museum in London | Zhang Y.,Key Laboratory of Plant Protection Resources and Pest Management
Zootaxa | Year: 2013

Two new species of Anidiocerus from China, A. brevispinus Xue & Zhang sp. nov. and A. longimus Xue & Zhang sp. nov. are described and illustrated, and A. variabilis Maldonado-Capriles, 1976 is placed as a senior synonym of A. flavidus Cai & Shen, 1998, syn. nov. A redescription of the genus is provided together with a checklist and key for the separation of males. Copyright © 2013 Magnolia Press. Source


Chen H.-M.,Key Laboratory of Plant Protection Resources and Pest Management | Hua B.-Z.,Key Laboratory of Plant Protection Resources and Pest Management
Zootaxa | Year: 2011

The morphology and chaetotaxy of the first instar larva of the scorpionfly Sinopanorpa tincta (Navás, 1931) are described and illustrated for the first time with the aid of light microscopy and scanning electron microscopy, with particular attention to the mouthparts and their sensilla. A comparison is made with the larvae of other known Mecoptera, especially Panorpa and Neopanorpa in Panorpidae. Copyright © 2011 Magnolia Press. Source


Hu Q.,Key Laboratory of Plant Protection Resources and Pest Management | Hu Q.,Northwest University, China | Feng J.,Key Laboratory of Plant Protection Resources and Pest Management | Feng J.,Northwest University, China
Transactions of the American Entomological Society | Year: 2011

Male and macropterous female of Ctenothrips smilax Bhatti, 1976 are first described and illustrated, together with brachypterous female which has already been reported by Bhatti in 1976. The species was firstly reported from China. All specimens are deposited in the Entomological Museum of Northwest A&F University (NWAFU). Source

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