Key Laboratory of Plant Protection Resources and Pest Management

Yangling, China

Key Laboratory of Plant Protection Resources and Pest Management

Yangling, China
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Gnezdilov V.M.,Russian Academy of Sciences | Bourgoin T.,CNRS Systematics, Biodiversity and Evolution Institute | Wang M.,Key Laboratory of Plant Protection Resources and Pest Management
Annales Zoologici | Year: 2017

Euxaldar jehucal Fennah, 1978 is redescribed. Euxaldar lenis sp. Nov. is described from Southern Vietnam. Sexual dimorphism and asymmetry in male genitalia are recorded for the genus Euxaldar Fennah, 1978. © Museum and Institute of Zoology PAS.


Xue Q.,Key Laboratory of Plant Protection Resources and Pest Management | Viraktamath C.A.,University of Agricultural Sciences, Bangalore | Zhang Y.,Key Laboratory of Plant Protection Resources and Pest Management
Entomologica Americana | Year: 2017

A checklist to Chinese Idiocerinae and a key to included genera is provided and Anidiocerus fasciatus Xue & Zhang, sp. nov. from China is described and illustrated. Megipocerus Zakhvatkin and its type species, Megipocerus mordvilkoi Zakhvatkin, are newly recorded from China, the genus is redescribed and the species figured.


Yang Z.,Key laboratory of Plant Protection Resources and Pest Management | Landry J.-F.,Agriculture and Agri Food Canada | Handfield L.,133 rue Messier and 301 | Zhang Y.,Key laboratory of Plant Protection Resources and Pest Management | And 6 more authors.
Systematic Entomology | Year: 2012

Anania coronata (Hufnagel), a Holarctic species of pyraustine crambid moth, has long been treated as having two geographically separated subspecies - the nominotypical Anania coronata in the Palaearctic Region and Anania coronata tertialis (Guenée) in the Nearctic Region. Maximum likelihood and Bayesian inference analysis of mitochondrial DNA barcodes both recover four well-supported, reciprocally monophyletic groups within Anania coronata. Qualitative and quantitative analyses of genital structures reveal diagnostic differences that correspond to the four barcode lineages. On the basis of both molecular and morphological evidence, we conclude that Anania coronata is actually a complex of four species. Anania coronata (Hufnagel) is restricted to Europe, whereas three species occur in North America: Anania tertialis (Guenée), Anania plectilis (Grote & Robinson) and Anania tennesseensis sp.n. Yang. © 2012 The Authors. Systematic Entomology © 2012 The Royal Entomological Society.


Feng J.-T.,Key Laboratory of Plant Protection Resources and Pest Management | Feng J.-T.,Northwest University, China | Wang H.,Key Laboratory of Plant Protection Resources and Pest Management | Ren S.-X.,Key Laboratory of Plant Protection Resources and Pest Management | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

Thirty-eight new ester derivatives of carabrol were designed, synthesized, and characterized by 1H and 13C NMR and HR-ESI-MS. Their antifungal activities against the fungal pathogen Colletotrichum lagenarium were evaluated using a spore germination assay. Of these 38 ester derivatives, 16 showed higher antifungal activity than that of carabrol and 7 showed higher antifungal activity than that of carabrone. It was found that the C-4 position of carabrol was a key position involving its antifungal activity, which showed the variation of 50% inhibition concentration (IC 50) from 2.70 to 52.33 μg/mL. When substituted by the phenyl ring, the ester derivatives with electron-attracting groups showed higher activity than those with electron-donating ones. Two ester derivatives, carabryl 4-cynaobenzoate (II-17, IC 50 2.70 μg/mL) and carabryl 4-isopropylbenzoate (II-27, IC 50 2.82 μg/mL), showed only slightly lower antifungal activity than that of the positive control chlorothalonil (IC 50 0.87 μg/mL) and have been identified as promising leads for development of new environmentally friendly fungicides. © 2012 American Chemical Society.


Cao Y.,Key Laboratory of Plant Protection Resources and Pest Management | Zhang Y.,Key Laboratory of Plant Protection Resources and Pest Management
Zootaxa | Year: 2014

The genera Gredzinskiya Dworakowska and Musbrnoia Dworakowska are revised and seven new species are described: Gredzinskiya lamellaris, G. serrata, G. spinalis, G. zhangae, Musbrnoia angusta, M. corollaris and M. lata spp. nov.. Habitus photos, illustrations of male genitalia and keys to adult males for both genera are provided. © Copyright 2014 Magnolia Press.


Chen H.-M.,Key Laboratory of Plant Protection Resources and Pest Management | Hua B.-Z.,Key Laboratory of Plant Protection Resources and Pest Management
Zootaxa | Year: 2011

The morphology and chaetotaxy of the first instar larva of the scorpionfly Sinopanorpa tincta (Navás, 1931) are described and illustrated for the first time with the aid of light microscopy and scanning electron microscopy, with particular attention to the mouthparts and their sensilla. A comparison is made with the larvae of other known Mecoptera, especially Panorpa and Neopanorpa in Panorpidae. Copyright © 2011 Magnolia Press.


Liu S.,Key Laboratory of Plant Protection Resources and Pest Management | Hua B.,Key Laboratory of Plant Protection Resources and Pest Management
Acta Zoologica | Year: 2010

The morphology, histology and ultrastructure of the salivary glands and salivary pumps in the scorpionfly Panorpa obtusaCheng 1949 were investigated using light microscopy and scanning and transmission electron microscopy. The salivary glands display a distinct sexual dimorphism. The female has only two small sac-like glands located in the prothorax, while the male possesses six long tubular glands extending into the sixth abdominal segment. The male salivary glands can be divided into five distinct regions. The apical long, thin secretory region possesses numerous secretory cells containing large secretory vesicles; the salivary reservoir expands in diameter, accumulating and temporarily storing the saliva in addition to secreting saliva; the constricted region contains prismatic cells with complex infolded plasma membrane; the sac has an internal brush border to absorb water and ions; the common salivary duct contains longitudinal muscles in the male, but not in the female. The salivary pump possesses independent strong dorsal muscles and abundant internal palm spines near its orifice. The anatomy and ultrastructure of the salivary glands and the salivary pump of scorpionflies as well as their possible functions are briefly discussed. © 2009 The Authors. Acta Zoologica © 2009 The Royal Swedish Academy of Sciences.


Wang J.,Key Laboratory of Plant Protection Resources and Pest Management
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

Bradysia hygida salivary glands antimicrobial peptide 1 (BhSGAMP-1) is one of the antimicrobial peptides involved in a preventive mechanism of defense of the fly Bradysia hygida. To know better about the molecular characterization of this antimicrobial peptide, we expressed and purified the modified BhSGAMP-1-S and investigated its antimicrobial activity. We synthesized the gene of BhSGAMP-1-S designed with preferred codons of Escherichia coli and expressed it as a fusion protein in E. coli TB1 by using pMAL-c2X as vector. We purified the fusion protein using amylase resin affinity chromatography. In addition, we cleaved the fusion protein by enterokinase and the released recombinant BhSGAMP-1-S was separated by size exclusion chromatography, then followed by reversed-phase high performance liquid chromatography. We analyzed the antimicrobial activities of the purified recombinant BhSGAMP-1-S by bioassays. The fusion protein was mostly expressed in soluble form under the optimized conditions. The recombinant BhSGAMP-1-S was produced with a pure yield of 0.38 mg/100 mL culture medium. Antimicrobial assays demonstrated that the recombinant BhSGAMP-1-S was active against several Gram-positive and Gram-negative bacteria and fungi. It appears to be the first successful production of the recombinant BhSGAMP-1-S from fly Bradysia hygida. Data presented here confirm that the recombinant BhSGAMP-1-S is now ready for further studying and characterizing their antimicrobial properties.


Zhang G.-H.,Key Laboratory of Plant Protection Resources and Pest Management | Li Y.-P.,Key Laboratory of Plant Protection Resources and Pest Management | Xu X.-L.,Key Laboratory of Plant Protection Resources and Pest Management | Chen H.,Key Laboratory of Plant Protection Resources and Pest Management | Wu J.-X.,Key Laboratory of Plant Protection Resources and Pest Management
Journal of Chemical Ecology | Year: 2012

Two novel general odorant-binding protein (GOBP) cDNAs (GmolGOBP1 and GmolGOBP2) were cloned and characterized from female antennal tissue of the oriental fruit moth, Grapholita molesta. We focused our investigation on this olfactory protein family by using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends-PCR (RACE-PCR). The full-length open reading frames of GmolGOBP1 and GmolGOBP2 were 492 and 483 nucleotides long, which encode 164 and 161 amino acid residue peptides, respectively. Protein signature analyses revealed that they each contained six conserved cysteines with an N-terminal signal sequence of 20 amino acids. The alignment of the two deduced protein sequences with other Lepidoptera GOBPs showed high sequence similarity (70-80 %) with other full-length sequences from GenBank. Sequence similarity between the two GOBPs was only 48 %, suggesting that the two proteins belong to different classes of lepidopteran GOBPs. RT-PCR analysis revealed that the two GOBP genes were expressed only in antennae of both sexes. Real-time PCR analysis further indicated that the transcript level of GmolGOBP1 was higher in males than in females, whereas the transcript level of GmolGOBP2 was higher in females than in males. Temporally, the two GOBP genes were expressed during the complete photoperiod (15L:9D). The highest transcript levels of GmolGOBP1 in both sexes and GmolGOBP2 in females were detected at the end of photophase and during scotophase. The expression of GmolGOBP2 in males remained at similar levels during the complete photoperiod. Based on these results, the possible physiological functions of GmolGOBPs are discussed. © Springer Science+Business Media, LLC 2012.


Sun Y.,Key Laboratory of Plant Protection Resources and Pest Management | Meng R.,Key Laboratory of Plant Protection Resources and Pest Management | Wang Y.,Key Laboratory of Plant Protection Resources and Pest Management
Zootaxa | Year: 2012

Neogergithoides gen. nov. is described from China with N. tubercularis sp. nov. as the type species. Morphological structures, male and female genitalia and female internal reproductive organs of the new species are described and illustrated. A key to genera of Hemisphaeriini is provided. The genus is compared to other superficially similar genera. Copyright © 2012 Magnolia Press.

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