Entity

Time filter

Source Type


Ma X.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Li C.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Wang M.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation
Bioengineered | Year: 2015

The transcription factor NUCLEAR FACTOR Y (NF-Y) plays an essential role in many developmental and stress-responsive processes in plants. NFY composed of 3 subunits, NF-YA, NFYB, and NF-YC, targets the CCAAT box, a common cis-element in eukaryotic promoters. We recently identified a gene TaNF-YA10–1 from the wheat salinity tolerant cultivar SR3 and found that recombinant TaNF-YA10–1 could successfully bind to the CCAAT motif in vitro. We also showed that the constitutive expression of TaNF-YA10–1 in Arabidopsis thaliana significantly increased the plant’s sensitivity to salinity. Here, we further demonstrated that TaNFYA10– 1 -overexpressing plants conferred drought tolerance as judged from the relative root length and whole-plant growth under drought stress. These results suggest that TaNF-YA10–1 functions independently in salinity and drought stress. Our findings are helpful in understanding the distinct roles of NF-YA in plant stress responses. © 2015 Taylor & Francis Group, LLC. Source


Zheng W.-W.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Yang D.-T.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Wang J.-X.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Song Q.-S.,University of Missouri | And 2 more authors.
Molecular and Cellular Endocrinology | Year: 2010

To probe the specific functions of the chaperone protein Hsc70 in 20-hydroxyecdysone signaling, we report on the roles of the Hsc70 from Helicoverpa armigera. RT-PCR analysis revealed that the genes for HaEcRB1 and HaUSP1 were upregulated in 5th molting and metamorphic molting larvae, whereas HaHsc70 maintained a constitutive expression level throughout larval development. Silencing HaEcRB1, HaUSP1 or HaHsc70 by RNAi inhibited the expression of a set of 20E-responsive genes. Immunocytochemical assay demonstrated that HaHsc70 is located predominantly in the cytoplasm of unstimulated cells and partially translocated to the nucleus after stimulation by 20E. Knockdown of HaHsc70 by RNAi decreased the amount of both HaEcRB1 and HaUSP1 in the nucleus. HaHsc70 was capable of binding to HaUSP1 in pull-down assays. These data suggest that Hsc70 participates in the 20E signal transduction pathway via binding to USP1 and mediating the expression of EcRB1, USP1 and then a set of 20E-responsive genes. © 2009 Elsevier Ireland Ltd. Source


Li Y.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Li Y.,Shandong University | Li P.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Li P.,Shandong University | And 7 more authors.
Planta | Year: 2014

Family-1 UDP glycosyltransferases (UGTs) from plants transfer sugar moieties from activated sugar donors to a wide range of small molecules, and control many metabolic processes during plant growth and development. Here, we report a genome-wide analysis of maize that identified 147 Family-1 glycosyltransferases based on their conserved PSPG motifs. Phylogenetic analysis of these genes with 18 Arabidopsis UGTs and two rice UGTs clustered them into 17 groups (A-Q). The patterns of intron gain/loss events, as well as their positions within UGTs from the same group, further aided elucidation of their divergence and evolutionary relationships between UGTs. Expression analysis of the maize UGT genes using both online microarray data and quantitative real-time PCR verification indicates that UGT genes are widely expressed in various tissues and likely play important roles in plant growth and development. Our study provides useful information on the Family-1 UGTs in maize, and will facilitate their further characterization to better understand their functions. © 2014 Springer-Verlag Berlin Heidelberg. Source


Jin S.-H.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Jin S.-H.,Shandong University | Ma X.-M.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Ma X.-M.,Shandong University | And 6 more authors.
Planta | Year: 2013

Trans-zeatin is a kind of cytokinins that plays a crucial role in plant growth and development. The master trans-zeatin O-glucosyltransferase of Arabidopsis thaliana, UGT85A1, has been previously identified through biochemical approach. To determine the in planta role of UGT85A1 gene, the characterization of transgenic Arabidopsis plants overexpressing UGT85A1 was carried out. Under normal conditions, transgenic Arabidopsis did not display clearly altered phenotypes. A remarkable alteration is that the accumulation level of the trans-zeatin O-glucosides was significantly increased in UGT85A1 overexpressing transgenic Arabidopsis, while other forms of cytokinins kept the similar concentrations compared to the wild type. When treated with exogenously applied trans-zeatin, UGT85A1 overexpressing Arabidopsis showed much less sensitivity to trans-zeatin in primary root elongation and lateral root formation. Meanwhile, the chlorophyll content of detached leaves of transgenic Arabidopsis was much lower than wild type. Studies of spatial-temporal expression patterns showed that UGT85A1 was mainly expressed in the early seedlings and developing seeds. Analysis of subcellular localization suggested that UGT85A1 was localized to cytoplasm and nucleus. Taken together, our data suggest that overexpression of Arabidopsis glucosyltransferase UGT85A1 influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation in planta. © 2012 Springer-Verlag Berlin Heidelberg. Source


Tian H.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Jia Y.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Niu T.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Yu Q.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation | Ding Z.,The Key Laboratory of Plant Cell Engineering and Germplasm Innovation
Plant Cell Reports | Year: 2014

Key message: The core regulators which are required for primary root growth and development also function in lateral root development or lateral root stem cell niche maintenance. Abstract: The primary root systems and the lateral root systems are the two important root systems which are vital to the survival of plants. Though the molecular mechanism of the growth and development of both the primary root systems and the lateral root systems have been extensively studied individually in Arabidopsis, there are not so much evidence to show that if both root systems share common regulatory mechanisms. AP2 family transcription factors such as PLT1 (PLETHORA1) and PLT2, GRAS family transcription factors such as SCR (SCARECROW) and SHR (SHORT ROOT) and WUSCHEL-RELATED HOMEOBOX transcription factor WOX5 have been extensively studied and found to be essential for primary root growth and development. In this study, through the expression pattern analysis and mutant examinations, we found that these core regulators also function in lateral root development or lateral root stem cell niche maintenance. © 2014 Springer-Verlag Berlin Heidelberg. Source

Discover hidden collaborations